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EPA Final Rule: Clean Water Act Methods Update Rule for the Analysis of Effluent

2024-04-16T05:00:00Z

The U.S. Environmental Protection Agency (EPA) is finalizing changes to its test procedures required to be used by industries and municipalities when analyzing the chemical, physical, and biological properties of wastewater and other samples for reporting under the EPA's National Pollutant Discharge Elimination System permit program. The Clean Water Act requires the EPA to promulgate these test procedures (analytical methods) for analysis of pollutants. The EPA anticipates that these changes will provide increased flexibility for the regulated community in meeting monitoring requirements while improving data quality. In addition, this update to the CWA methods will incorporate technological advances in analytical technology and make a series of minor changes and corrections to existing approved methods. As such, the EPA expects that these changes will not result in any negative economic impacts.

DATES: This final rule is effective on June 17, 2024, published in the Federal Register April 16, 2024, page 27288.

View final rule.

§136.3 Identification of test procedures.
(a), tables IA, IB, IC, ID, and IHRevisedView text
(b)RevisedView text
(e), table II, Footnote “5”RevisedView text

New Text

§136.3 Identification of test procedures.

(a)

* * * *

Table IA—List of Approved Biological Methods for Wastewater and Sewage Sludge
Parameter and unitsMethod 1EPAStandard methodsAOAC, ASTM, USGSOther
Table IA notes:
1 The method must be specified when results are reported.
2 A 0.45-µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of extractables which could interfere with their growth.
3 Microbiological Methods for Monitoring the Environment, Water and Wastes, EPA/600/8-78/017. 1978. US EPA.
4 U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS.
5 Because the MF technique usually yields low and variable recovery from chlorinated wastewaters, the Most Probable Number method will be required to resolve any controversies.
6 Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample.
7 When the MF method has been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and comparability of results.
8 To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines.
9 Annual Book of ASTM Standards—Water and Environmental Technology, Section 11.02. 2000, 1999, 1996. ASTM International.
10 Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, 1998. AOAC International.
11 Recommended for enumeration of target organism in sewage sludge.
12 The multiple-tube fermentation test is used in 9221B.2-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis.
13 These tests are collectively known as defined enzyme substrate tests.
14 After prior enrichment in a presumptive medium for total coliform using 9221B.2-2014, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 µg/mL of MUG may be used.
15 Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using Lauryl-Tryptose Broth (LTB) and EC Medium, EPA-821-R-14-009. September 2014. U.S. EPA.
16 Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert® may be enumerated with the multiple-well procedures, Quanti-Tray® or Quanti-Tray®/2000 and the MPN calculated from the table provided by the manufacturer.
17 Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35°C rather than the 24 h required for the Colilert® test and is recommended for marine water samples.
18 Descriptions of the Colilert®, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories, Inc.
19 A description of the mColiBlue24® test is available from Hach Company.
20 Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using A-1 Medium, EPA-821-R-06-013. July 2006. U.S. EPA.
21 Method 1603.1: Escherichia coli ( E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-23-008. September 2023. U.S. EPA.
22 Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium, EPA-821-R-14-012. September 2014. U.S. EPA.
23 A description of the Enterolert® test may be obtained from IDEXX Laboratories Inc.
24 Method 1600.1: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-23-006. September 2023. U.S. EPA.
25 Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, EPA-821-R-02-012. Fifth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
26 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013. Fourth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
27 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, EPA-821-R-02-014. Third Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
28 To use Colilert-18® to assay for fecal coliforms, the incubation temperature is 44.5 ± 0.2 °C, and a water bath incubator is used.
29 On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources.
30 On a monthly basis, at least ten sheen colonies from positive samples must be verified using lauryl tryptose broth and brilliant green lactose bile broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using lauryl tryptose broth. Where possible, verifications should be done from randomized sample sources.
31 Subject coliform positive samples determined by 9222 B-2015 or other membrane filter procedure to 9222 I-2015 using NA-MUG media.
32 Verification of colonies by incubation of BHI agar at 10 ± 0.5 °C for 48 ± 3 h is optional. As per the Errata to the 23rd Edition of Standard Methods for the Examination of Water and Wastewater “Growth on a BHI agar plate incubated at 10 ± 0.5 °C for 48 ± 3 h is further verification that the colony belongs to the genus Enterococcus.”
33 9221F. 2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted vials collect gas produced by thermotolerant fecal coliforms.
Bacteria
1. Coliform (fecal), number per gram dry weightMost Probable Number (MPN), 5 tube, 3 dilution, orp. 132, 3 1680, 1115 1681 11209221 E-2014.
Membrane filter (MF), 25 single stepp. 124 39222 D-2015. 29
2. Coliform (fecal), number per 100 mLMPN, 5 tube, 3 dilution, orp. 132 39221 E-2014, 9221 F-2014. 33
Multiple tube/multiple well, orColilert-18®. 131828
MF, 25 single step 5p. 124 39222 D-2015 29B-0050-85. 4
3. Coliform (total), number per 100 mLMPN, 5 tube, 3 dilution, orp. 114 39221 B-2014.
MF, 25 single step orp. 108 39222 B-2015 30B-0025-85. 4
MF, 25 two step with enrichmentp. 111 39222 B-2015. 30
4. E. coli, number per 100 mLMPN 6816 multiple tube, or9221 B2014/9221 F-2014. 121433
multiple tube/multiple well, or9223 B-2016 13991.15 10Colilert®. 1318 Colilert-18®. 131718
MF, 25678 two step, or9222 B-2015/9222 I-2015. 31
Single step1603.1 21m-ColiBlue24®. 19
5. Fecal streptococci, number per 100 mLMPN, 5 tube, 3 dilution, orp. 139 39230 B-2013.
MF, 2 orp. 136 39230 C-2013 32B-0055-85. 4
Plate countp. 143. 3
6. Enterococci, number per 100 mLMPN, 5 tube, 3 dilution, orp. 139 39230 B-2013.
MPN, 68 multiple tube/multiple well, or9230 D-2013D6503-99 9Enterolert®. 1323
MF 25678 single step or1600.1 249230 C-2013. 32
Plate countp. 143. 3
7. Salmonella , number per gram dry weight 11MPN multiple tube1682. 22
Aquatic Toxicity
8. Toxicity, acute, fresh water organisms, LC 50 , percent effluentWater flea, Cladoceran, Ceriodaphnia dubia acute2002.0. 25
Water flea, Cladocerans, Daphnia pulex and Daphnia magna acute2021.0. 25
Fish, Fathead minnow, Pimephales promelas, and Bannerfin shiner, Cyprinella leedsi, acute2000.0. 25
Fish, Rainbow trout, Oncorhynchus mykiss, and brook trout, Salvelinus fontinalis, acute2019.0. 25
9. Toxicity, acute, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, LC 50 , percent effluentMysid, Mysidopsis bahia, acute2007.0. 25
Fish, Sheepshead minnow, Cyprinodon variegatus, acute2004.0. 25
Fish, Silverside, Menidia beryllina, Menidia menidia, and Menidia peninsulae, acute2006.0. 25
10. Toxicity, chronic, fresh water organisms, NOEC or IC 25 , percent effluentFish, Fathead minnow, Pimephales promelas, larval survival and growth1000.0. 26
Fish, Fathead minnow, Pimephales promelas, embryo-larval survival and teratogenicity1001.0. 26
Water flea, Cladoceran, Ceriodaphnia dubia, survival and reproduction1002.0. 26
Green alga, Selenastrum capricornutum, growth1003.0. 26
11. Toxicity, chronic, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, NOEC or IC 25 , percent effluentFish, Sheepshead minnow, Cyprinodon variegatus, larval survival and growth.1004.0. 27
Fish, Sheepshead minnow, Cyprinodon variegatus, embryo-larval survival and teratogenicity1005.0. 27
Fish, Inland silverside, Menidia beryllina, larval survival and growth1006.0. 27
Mysid, Mysidopsis bahia, survival, growth, and fecundity1007.0. 27
Sea urchin, Arbacia punctulata, fertilization1008.0. 27

Table IB—List of Approved Inorganic Test Procedures
ParameterMethodology 58EPA 52Standard methods 84ASTMUSGS/AOAC/Other
Table IB Notes:
1 Methods for Chemical Analysis of Water and Wastes, EPA-600/4-79-020. Revised March 1983 and 1979, where applicable. U.S. EPA.
2 Methods for Analysis of Inorganic Substances in Water and Fluvial Sediments, Techniques of Water-Resource Investigations of the U.S. Geological Survey, Book 5, Chapter A1., unless otherwise stated. 1989. USGS.
3 Official Methods of Analysis of the Association of Official Analytical Chemists, Methods Manual, Sixteenth Edition, 4th Revision, 1998. AOAC International.
4 For the determination of total metals (which are equivalent to total recoverable metals) the sample is not filtered before processing. A digestion procedure is required to solubilize analytes in suspended material and to break down organic-metal complexes (to convert the analyte to a detectable form for colorimetric analysis). For non-platform graphite furnace atomic absorption determinations, a digestion using nitric acid (as specified in Section 4.1.3 of Methods for Chemical Analysis of Water and Wastes) is required prior to analysis. The procedure used should subject the sample to gentle acid refluxing, and at no time should the sample be taken to dryness. For direct aspiration flame atomic absorption (FLAA) determinations, a combination acid (nitric and hydrochloric acids) digestion is preferred, prior to analysis. The approved total recoverable digestion is described as Method 200.2 in Supplement I of “Methods for the Determination of Metals in Environmental Samples” EPA/600R-94/111, May 1994, and is reproduced in EPA Methods 200.7, 200.8, and 200.9 from the same Supplement. However, when using the gaseous hydride technique or for the determination of certain elements such as antimony, arsenic, selenium, silver, and tin by non-EPA graphite furnace atomic absorption methods, mercury by cold vapor atomic absorption, the noble metals and titanium by FLAA, a specific or modified sample digestion procedure may be required, and, in all cases the referenced method write-up should be consulted for specific instruction and/or cautions. For analyses using inductively coupled plasma-atomic emission spectrometry (ICP-AES), the direct current plasma (DCP) technique or EPA spectrochemical techniques (platform furnace AA, ICP-AES, and ICP-MS), use EPA Method 200.2 or an approved alternate procedure ( e.g., CEM microwave digestion, which may be used with certain analytes as indicated in this table IB); the total recoverable digestion procedures in EPA Methods 200.7, 200.8, and 200.9 may be used for those respective methods. Regardless of the digestion procedure, the results of the analysis after digestion procedure are reported as “total” metals.
5 Copper sulfate or other catalysts that have been found suitable may be used in place of mercuric sulfate.
6 Manual distillation is not required if comparability data on representative effluent samples are on file to show that this preliminary distillation step is not necessary; however, manual distillation will be required to resolve any controversies. In general, the analytical method should be consulted regarding the need for distillation. If the method is not clear, the laboratory may compare a minimum of 9 different sample matrices to evaluate the need for distillation. For each matrix, a matrix spike and matrix spike duplicate are analyzed both with and without the distillation step (for a total of 36 samples, assuming 9 matrices). If results are comparable, the laboratory may dispense with the distillation step for future analysis. Comparable is defined as <20% RPD for all tested matrices). Alternatively, the two populations of spike recovery percentages may be compared using a recognized statistical test.
7 Industrial Method Number 379-75 WE Ammonia, Automated Electrode Method, Technicon Auto Analyzer II. February 19, 1976. Bran & Luebbe Analyzing Technologies Inc.
8 The approved method is that cited in Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1979. USGS.
9 American National Standard on Photographic Processing Effluents. April 2, 1975. American National Standards Institute.
10 In-Situ Method 1003-8-2009, Biochemical Oxygen Demand (BOD) Measurement by Optical Probe. 2009. In-Situ Incorporated.
11 The use of normal and differential pulse voltage ramps to increase sensitivity and resolution is acceptable.
12 Carbonaceous biochemical oxygen demand (CBOD 5) must not be confused with the traditional BOD 5 test method which measures “total 5-day BOD.” The addition of the nitrification inhibitor is not a procedural option but must be included to report the CBOD 5 parameter. A discharger whose permit requires reporting the traditional BOD 5 may not use a nitrification inhibitor in the procedure for reporting the results. Only when a discharger's permit specifically states CBOD 5 is required can the permittee report data using a nitrification inhibitor.
13 OIC Chemical Oxygen Demand Method. 1978. Oceanography International Corporation.
14 Method 8000, Chemical Oxygen Demand, Hach Handbook of Water Analysis, 1979. Hach Company.
15 The back-titration method will be used to resolve controversy.
16 Orion Research Instruction Manual, Residual Chlorine Electrode Model 97-70. 1977. Orion Research Incorporated. The calibration graph for the Orion residual chlorine method must be derived using a reagent blank and three standard solutions, containing 0.2, 1.0, and 5.0 mL 0.00281 N potassium iodate/100 mL solution, respectively.
17 Method 245.7, Mercury in Water by Cold Vapor Atomic Fluorescence Spectrometry, EPA-821-R-05-001. Revision 2.0, February 2005. US EPA.
18 National Council of the Paper Industry for Air and Stream Improvement (NCASI) Technical Bulletin 253 (1971) and Technical Bulletin 803, May 2000.
19 Method 8506, Bicinchoninate Method for Copper, Hach Handbook of Water Analysis. 1979. Hach Company.
20 When using a method with block digestion, this treatment is not required.
21 Industrial Method Number 378-75WA, Hydrogen ion (pH) Automated Electrode Method, Bran & Luebbe (Technicon) Autoanalyzer II. October 1976. Bran & Luebbe Analyzing Technologies.
22 Method 8008, 1,10-Phenanthroline Method using FerroVer Iron Reagent for Water. 1980. Hach Company.
23 Method 8034, Periodate Oxidation Method for Manganese, Hach Handbook of Wastewater Analysis. 1979. Hach Company.
24 Methods for Analysis of Organic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A3, (1972 Revised 1987). 1987. USGS.
25 Method 8507, Nitrogen, Nitrite-Low Range, Diazotization Method for Water and Wastewater. 1979. Hach Company.
26 Just prior to distillation, adjust the sulfuric-acid-preserved sample to pH 4 with 1 + 9 NaOH.
27 The colorimetric reaction must be conducted at a pH of 10.0 ± 0.2.
28 Addison, R.F., and R.G. Ackman. 1970. Direct Determination of Elemental Phosphorus by Gas-Liquid Chromatography, Journal of Chromatograph y, 47(3):421-426.
29 Approved methods for the analysis of silver in industrial wastewaters at concentrations of 1 mg/L and above are inadequate where silver exists as an inorganic halide. Silver halides such as the bromide and chloride are relatively insoluble in reagents such as nitric acid but are readily soluble in an aqueous buffer of sodium thiosulfate and sodium hydroxide to pH of 12. Therefore, for levels of silver above 1 mg/L, 20 mL of sample should be diluted to 100 mL by adding 40 mL each of 2 M Na 2 S 2 O 3 and NaOH. Standards should be prepared in the same manner. For levels of silver below 1 mg/L the approved method is satisfactory.
30 The use of EDTA decreases method sensitivity. Analysts may omit EDTA or replace with another suitable complexing reagent provided that all method-specified quality control acceptance criteria are met.
31 For samples known or suspected to contain high levels of silver ( e.g., in excess of 4 mg/L), cyanogen iodide should be used to keep the silver in solution for analysis. Prepare a cyanogen iodide solution by adding 4.0 mL of concentrated NH 4 OH, 6.5 g of KCN, and 5.0 mL of a 1.0 N solution of I 2 to 50 mL of reagent water in a volumetric flask and dilute to 100.0 mL. After digestion of the sample, adjust the pH of the digestate to <7 to prevent the formation of HCN under acidic conditions. Add 1 mL of the cyanogen iodide solution to the sample digestate and adjust the volume to 100 mL with reagent water (NOT acid). If cyanogen iodide is added to sample digestates, then silver standards must be prepared that contain cyanogen iodide as well. Prepare working standards by diluting a small volume of a silver stock solution with water and adjusting the pH>7 with NH 4 OH. Add 1 mL of the cyanogen iodide solution and let stand 1 hour. Transfer to a 100-mL volumetric flask and dilute to volume with water.
32 “Water Temperature-Influential Factors, Field Measurement and Data Presentation,” Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 1, Chapter D1. 1975. USGS.
33 Method 8009, Zincon Method for Zinc, Hach Handbook of Water Analysis, 1979. Hach Company.
34 Method AES0029, Direct Current Plasma (DCP) Optical Emission Spectrometric Method for Trace Elemental Analysis of Water and Wastes. 1986—Revised 1991. Thermo Jarrell Ash Corporation.
35 In-Situ Method 1004-8-2009, Carbonaceous Biochemical Oxygen Demand (CBOD) Measurement by Optical Probe. 2009. In-Situ Incorporated.
36 Microwave-assisted digestion may be employed for this metal, when analyzed by this methodology. Closed Vessel Microwave Digestion of Wastewater Samples for Determination of Metals. April 16, 1992. CEM Corporation.
37 When determining boron and silica, only plastic, PTFE, or quartz laboratory ware may be used from start until completion of analysis.
38 Only use n -hexane ( n -Hexane—85% minimum purity, 99.0% min. saturated C6 isomers, residue less than 1 mg/L) extraction solvent when determining Oil and Grease parameters—Hexane Extractable Material (HEM), or Silica Gel Treated HEM (analogous to EPA Methods 1664 Rev. A and 1664 Rev. B). Use of other extraction solvents is prohibited.
39 Method PAI-DK01, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Titrimetric Detection. Revised December 22, 1994. OI Analytical.
40 Method PAI-DK02, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Colorimetric Detection. Revised December 22, 1994. OI Analytical.
41 Method PAI-DK03, Nitrogen, Total Kjeldahl, Block Digestion, Automated FIA Gas Diffusion. Revised December 22, 1994. OI Analytical.
42 Method 1664 Rev. B is the revised version of EPA Method 1664 Rev. A. U.S. EPA. February 1999, Revision A. Method 1664, n -Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n -Hexane Extractable Material (SGT-HEM; Non-polar Material) by Extraction and Gravimetry. EPA-821-R-98-002. U.S. EPA. February 2010, Revision B. Method 1664, n -Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n -Hexane Extractable Material (SGT-HEM; Non-polar Material) by Extraction and Gravimetry. EPA-821-R-10-001.
43 Method 1631, Revision E, Mercury in Water by Oxidation, Purge and Trap, and Cold Vapor Atomic Fluorescence Spectrometry, EPA-821-R-02-019. Revision E. August 2002, U.S. EPA. The application of clean techniques described in EPA's Method 1669: Sampling Ambient Water for Trace Metals at EPA Water Quality Criteria Levels, EPA-821-R-96-011, are recommended to preclude contamination at low-level, trace metal determinations.
44 Method OIA-1677-09, Available Cyanide by Ligand Exchange and Flow Injection Analysis (FIA). 2010. OI Analytical.
45 Open File Report 00-170, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Ammonium Plus Organic Nitrogen by a Kjeldahl Digestion Method and an Automated Photometric Finish that Includes Digest Cleanup by Gas Diffusion. 2000. USGS.
46 Open File Report 93-449, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Chromium in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1993. USGS.
47 Open File Report 97-198, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Molybdenum by Graphite Furnace Atomic Absorption Spectrophotometry. 1997. USGS.
48 Open File Report 92-146, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Total Phosphorus by Kjeldahl Digestion Method and an Automated Colorimetric Finish That Includes Dialysis. 1992. USGS.
49 Open File Report 98-639, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Arsenic and Selenium in Water and Sediment by Graphite Furnace-Atomic Absorption Spectrometry. 1999. USGS.
50 Open File Report 98-165, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Elements in Whole-water Digests Using Inductively Coupled Plasma-Optical Emission Spectrometry and Inductively Coupled Plasma-Mass Spectrometry. 1998. USGS.
51 Open File Report 93-125, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. USGS.
52 Unless otherwise indicated, all EPA methods, excluding EPA Method 300.1, are published in U.S. EPA. May 1994. Methods for the Determination of Metals in Environmental Samples, Supplement I, EPA/600/R-94/111; or U.S. EPA. August 1993. Methods for the Determination of Inorganic Substances in Environmental Samples, EPA/600/R-93/100. EPA Method 300.1 is U.S. EPA. Revision 1.0, 1997, including errata cover sheet April 27, 1999. Determination of Inorganic Ions in Drinking Water by Ion Chromatography.
53 Styrene divinyl benzene beads ( e.g., AMCO-AEPA-1 or equivalent) and stabilized formazin ( e.g., Hach StablCal TM or equivalent) are acceptable substitutes for formazin.
54 Waters Corp. Now included in ASTM D6508-15, Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. 2015.
55 Kelada-01, Kelada Automated Test Methods for Total Cyanide, Acid Dissociable Cyanide, and Thiocyanate, EPA 821-B-01-009, Revision 1.2, August 2001. US EPA. Note: A 450-W UV lamp may be used in this method instead of the 550-W lamp specified if it provides performance within the quality control (QC) acceptance criteria of the method in a given instrument. Similarly, modified flow cell configurations and flow conditions may be used in the method, provided that the QC acceptance criteria are met.
56 QuikChem Method 10-204-00-1-X, Digestion and Distillation of Total Cyanide in Drinking and Wastewaters using MICRO DIST and Determination of Cyanide by Flow Injection Analysis. Revision 2.2, March 2005. Lachat Instruments.
57 When using sulfide removal test procedures described in EPA Method 335.4, reconstitute particulate that is filtered with the sample prior to distillation.
58 Unless otherwise stated, if the language of this table specifies a sample digestion and/or distillation “followed by” analysis with a method, approved digestion and/or distillation are required prior to analysis.
59 Samples analyzed for available cyanide using OI Analytical method OIA-1677-09 or ASTM method D6888-16 that contain particulate matter may be filtered only after the ligand exchange reagents have been added to the samples, because the ligand exchange process converts complexes containing available cyanide to free cyanide, which is not removed by filtration. Analysts are further cautioned to limit the time between the addition of the ligand exchange reagents and sample filtration to no more than 30 minutes to preclude settling of materials in samples.
60 Analysts should be aware that pH optima and chromophore absorption maxima might differ when phenol is replaced by a substituted phenol as the color reagent in Berthelot Reaction (“phenol-hypochlorite reaction”) colorimetric ammonium determination methods. For example, when phenol is used as the color reagent, pH optimum and wavelength of maximum absorbance are about 11.5 and 635 nm, respectively—see, Patton, C.J. and S.R. Crouch. March 1977. Anal. Chem. 49:464-469. These reaction parameters increase to pH > 12.6 and 665 nm when salicylate is used as the color reagent—see, Krom, M.D. April 1980. The Analyst 105:305-316.
61 If atomic absorption or ICP instrumentation is not available, the aluminon colorimetric method detailed in the 19th Edition of Standard Methods for the Examination of Water and Wastewater may be used. This method has poorer precision and bias than the methods of choice.
62 Easy (1-Reagent) Nitrate Method, Revision November 12, 2011. Craig Chinchilla.
63 Hach Method 10360, Luminescence Measurement of Dissolved Oxygen in Water and Wastewater and for Use in the Determination of BOD 5 and CBOD 5 . Revision 1.2, October 2011. Hach Company. This method may be used to measure dissolved oxygen when performing the methods approved in this table IB for measurement of biochemical oxygen demand (BOD) and carbonaceous biochemical oxygen demand (CBOD).
64 In-Situ Method 1002-8-2009, Dissolved Oxygen (DO) Measurement by Optical Probe. 2009. In-Situ Incorporated.
65 Mitchell Method M5331, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Leck Mitchell.
66 Mitchell Method M5271, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Leck Mitchell.
67 Orion Method AQ4500, Determination of Turbidity by Nephelometry. Revision 5, March 12, 2009. Thermo Scientific.
68 EPA Method 200.5, Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry, EPA/600/R-06/115. Revision 4.2, October 2003. US EPA.
69 Method 1627, Kinetic Test Method for the Prediction of Mine Drainage Quality, EPA-821-R-09-002. December 2011. US EPA.
70 Techniques and Methods Book 5-B1, Determination of Elements in Natural-Water, Biota, Sediment and Soil Samples Using Collision/Reaction Cell Inductively Coupled Plasma-Mass Spectrometry, Chapter 1, Section B, Methods of the National Water Quality Laboratory, Book 5, Laboratory Analysis, 2006. USGS.
71 Water-Resources Investigations Report 01-4132, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Organic Plus Inorganic Mercury in Filtered and Unfiltered Natural Water with Cold Vapor-Atomic Fluorescence Spectrometry, 2001. USGS.
72 USGS Techniques and Methods 5-B8, Chapter 8, Section B, Methods of the National Water Quality Laboratory Book 5, Laboratory Analysis, 2011 USGS.
73 NECi Method N07-0003, “Nitrate Reductase Nitrate-Nitrogen Analysis,” Revision 9.0, March 2014, The Nitrate Elimination Co., Inc.
74 Timberline Instruments, LLC Method Ammonia-001, “Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Conductivity Cell Analysis,” June 2011, Timberline Instruments, LLC.
75 Hach Company Method 10206, “Spectrophotometric Measurement of Nitrate in Water and Wastewater,” Revision 2.1, January 2013, Hach Company.
76 Hach Company Method 10242, “Simplified Spectrophotometric Measurement of Total Kjeldahl Nitrogen in Water and Wastewater,” Revision 1.1, January 2013, Hach Company.
77 National Council for Air and Stream Improvement (NCASI) Method TNTP-W10900, “Total (Kjeldahl) Nitrogen and Total Phosphorus in Pulp and Paper Biologically Treated Effluent by Alkaline Persulfate Digestion,” June 2011, National Council for Air and Stream Improvement, Inc.
78 The pH adjusted sample is to be adjusted to 7.6 for NPDES reporting purposes.
79 I-2057-85 in U.S. Geological Survey Techniques of Water-Resources Investigations, Book 5, Chap. A1, Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, 1989.
80 Methods I-2522-90, I-2540-90, and I-2601-90 in U.S. Geological Survey Open-File Report 93-125, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments, 1993.
81 Method I-4472-97 in U.S. Geological Survey Open-File Report 98-165, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments, 1998.
82 FIAlab 100, “Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Fluorescence Detector Analysis”, April 4, 2018, FIAlab Instruments, Inc.
83 MACHEREY-NAGEL GmbH and Co. Method 036/038 NANOCOLOR® COD LR/HR, “Spectrophotometric Measurement of Chemical Oxygen Demand in Water and Wastewater”, Revision 1.5, May 2018, MACHEREY-NAGEL GmbH and Co. KG.
84 Please refer to the following applicable Quality Control Sections: Part 2000 Methods, Physical and Aggregate Properties 2020 (2021); Part 3000 Methods, Metals, 3020 (2021); Part 4000 Methods, Inorganic Nonmetallic Constituents, 4020 (2022); Part 5000 Methods, and Aggregate Organic Constituents, 5020 (2022). These Quality Control Standards are available for download at www.standardmethods.org at no charge.
85 Each laboratory may establish its own control limits by performing at least 25 glucose-glutamic acid (GGA) checks over several weeks or months and calculating the mean and standard deviation. The laboratory may then use the mean ± 3 standard deviations as the control limit for future GGA checks. However, GGA acceptance criteria can be no wider than 198 ± 30.5 mg/L for BOD 5 . GGA acceptance criteria for CBOD must be either 198 ± 30.5 mg/L, or the lab may develop control charts under the following conditions: dissolved oxygen uptake from the seed contribution is between 0.6-1.0 mg/L; control charts are performed on at least 25 GGA checks with three standard deviations from the derived mean; the RSD must not exceed 7.5%; and any single GGA value cannot be less than 150 mg/L or higher than 250 mg/L.
86 The approved method is that cited in Standard Methods for the Examination of Water and Wastewater, 14th Edition, 1976.
1. Acidity (as CaCO 3), mg/LElectrometric endpoint or phenolphthalein endpoint2310 B-2020D1067-16I-1020-85. 2
2. Alkalinity (as CaCO 3), mg/LElectrometric or Colorimetric titration to pH 4.5, Manual2320 B-2021D1067-16973.43, 3 I-1030-85. 2
Automatic310.2 (Rev. 1974) 1I-2030-85. 2
3. Aluminum—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 D-2019 or 3111 E-2019I-3051-85. 2
AA furnace3113 B-2020.
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97, 81
Direct Current Plasma (DCP) 36D4190-15See footnote. 34
Colorimetric (Eriochrome cyanine R)3500-Al B-2020.
4. Ammonia (as N), mg/LManual distillation 6 or gas diffusion (pH > 11), followed by any of the following:350.1 Rev. 2.0 (1993)4500-NH 3 B-2021973.49. 3
NesslerizationD1426-15 (A)973.49, 3 I-3520-85. 2
Titration4500-NH 3 C-2021.
Electrode4500-NH 3 D-2021 or E-2021D1426-15 (B)
Manual phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods4500-NH 3 F-2021See footnote. 60
Automated phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods350.1, 30 Rev. 2.0 (1993)4500-NH 3 G-2021, 4500-NH 3 H-2021I-4523-85, 2 I-2522-90. 80
Automated electrodeSee footnote. 7
Ion ChromatographyD6919-17.
Automated gas diffusion, followed by conductivity cell analysisTimberline Ammonia-001. 74
Automated gas diffusion followed by fluorescence detector analysisFIAlab100. 82
5. Antimony—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019.
AA furnace3113 B-2020.
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20.
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
6. Arsenic—Total, 4 mg/LDigestion, 4 followed by any of the following:206.5 (Issued 1978). 1
AA gaseous hydride3114 B-2020 or 3114 C-2020D2972-15 (B)I-3062-85. 2
AA furnace3113 B-2020D2972-15 (C)I-4063-98. 49
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5, Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20.
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05. 70
Colorimetric (SDDC)3500-As B-2020D2972-15 (A)I-3060-85. 2
7. Barium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 D-2019I-3084-85. 2
AA furnace3113 B-2020D4382-18.
ICP/AES 36200.5, Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCP 36See footnote. 34
8. Beryllium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 D-2019 or 3111 E-2019D3645-15 (A)I-3095-85. 2
AA furnace3113 B-2020D3645-15 (B).
STGFAA200.9, Rev. 2.2 (1994).
ICP/AES200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCPD4190-15See footnote. 34
Colorimetric (aluminon)See footnote 61
9. Biochemical oxygen demand (BOD 5), mg/LDissolved Oxygen Depletion5210 B-2016 85973.44 3 p. 17, 9 I-1578-78, 8 see footnote. 1063
10. Boron—Total, 37 mg/LColorimetric (curcumin)4500-B B-2011I-3112-85. 2
ICP/AES200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPD4190-15See footnote. 34
11. Bromide, mg/LElectrodeD1246-16I-1125-85. 2
Ion Chromatography300.0 Rev 2.1 (1993), and 300.1 Rev 1.0 (1997)4110 B-2020, C-2020 or D-2020D4327-17993.30, 3 I-2057-85. 79
CIE/UV4140 B-2020D6508-15D6508 Rev. 2. 54
12. Cadmium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D3557-17 (A or B)974.27 3 p. 37, 9 I-3135-85 2 or I-3136-85. 2
AA furnace3113 B-2020D3557-17 (D)I-4138-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-1472-85 2 or I-4471-97. 50
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Voltammetry 11D3557-17 (C).
Colorimetric (Dithizone)3500-Cd D-1990.
13. Calcium—Total, 4 mg/LDigestion 4 followed by any of the following:
AA direct aspiration3111 B-2019 or 3111 D-2019D511-14 (B)I-3152-85. 2
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPSee footnote. 34
Titrimetric (EDTA)3500-Ca B-2020D511-14 (A).
Ion ChromatographyD6919-17.
14. Carbonaceous biochemical oxygen demand (CBOD 5), mg/L 12Dissolved Oxygen Depletion with nitrification inhibitor5210 B-2016 85See footnotes. 35 63
15. Chemical oxygen demand (COD), mg/LTitrimetric410.3 (Rev. 1978) 15220 B-2011 or C-2011D1252-06(12) (A)973.46 3 p. 17, 9 I-3560-85. 2
Spectrophotometric, manual or automatic410.4 Rev. 2.0 (1993)5220 D-2011D1252-06(12) (B)See footnotes, 131483 I-3561-85. 2
16. Chloride, mg/LTitrimetric: (silver nitrate)4500-Cl B-2021D512-12 (B)I-1183-85. 2
(Mercuric nitrate)4500-Cl C-2021D512-12 (A)973.51, 3 I-1184-85. 2
Colorimetric: manualI-1187-85. 2
Automated (ferricyanide)4500-Cl E-2021I-2187-85. 2
Potentiometric Titration4500-Cl D-2021.
Ion Selective ElectrodeD512-12 (C).
Ion Chromatography300.0 Rev 2.1 (1993), and 300.1 Rev 1.0 (1997)4110 B-2020 or 4110 C-2020D4327-17993.30, 3 I-2057-90. 51
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
17. Chlorine—Total residual, mg/LAmperometric direct4500-Cl D-2011D1253-14.
Amperometric direct (low level)4500-Cl E-2011.
Iodometric direct4500-Cl B-2011.
Back titration ether end-point 154500-Cl C-2011.
DPD-FAS4500-Cl F-2011.
Spectrophotometric, DPD4500-Cl G-2011.
ElectrodeSee footnote. 16
17A. Chlorine—Free Available, mg/LAmperometric direct4500-Cl D-2011D1253-14.
Amperometric direct (low level)4500-Cl E-2011.
DPD-FAS4500-Cl F-2011.
Spectrophotometric, DPD4500-Cl G-2011.
18. Chromium VI dissolved, mg/L0.45-micron filtration followed by any of the following:
AA chelation-extraction3111 C-2019I-1232-85. 2
Colorimetric (diphenyl-carbazide)3500-Cr B-2020D1687-17 (A)I-1230-85. 2
19. Chromium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019D1687-17 (B)974.27, 3 I-3236-85. 2
AA chelation-extraction3111 C-2019.
AA furnace3113 B-2020D1687-17 (C)I-3233-93. 46
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20.
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05 70 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Colorimetric (diphenyl-carbazide)3500-Cr B-2020.
20. Cobalt—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019 or 3111 C-2019D3558-15 (A or B)p. 37, 9 I-323985. 2
AA furnace3113 B-2020D3558-15 (C)I-4243-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05 70 I-4472-97. 81
DCPD4190-15See footnote. 34
21. Color, platinum cobalt units or dominant wavelength, hue, luminance purityColorimetric (ADMI)2120 F-2021. 78
Platinum cobalt visual comparison2120 B-2021I-1250-85. 2
SpectrophotometricSee footnote. 18
22. Copper—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D1688-17 (A or B)974.27, 3 p. 37, 9 I-3270-85 2 or I-3271-85. 2
AA furnace3113 B-2020D1688-17 (C)I-4274-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05, 70 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Colorimetric (Neocuproine)3500-Cu B-2020.
Colorimetric (Bathocuproine)3500-Cu C-2020See footnote. 19
23. Cyanide—Total, mg/LAutomated UV digestion/distillation and ColorimetryKelada-01. 55
Segmented Flow Injection, In-Line Ultraviolet Digestion, followed by gas diffusion amperometry4500-CN P-2021D7511-12 (17).
Manual distillation with MgCl 2 , followed by any of the following:335.4 Rev. 1.0 (1993) 574500-CN B-2021 and C-2021D2036-09(15)(A), D7284-2010-204-00-1-X. 56
Flow Injection, gas diffusion amperometryD2036-09(15)(A) D7284-20.
Titrimetric4500-CN D-2021D2036-09(15)(A)See footnote 9 p. 22.
Spectrophotometric, manual4500-CN E-2021D2036-09(15)(A)I-3300-85. 2
Semi-Automated 20335.4 Rev. 1.0 (1993) 574500-CN N-202110-204-00-1-X, 56 I-4302-85. 2
Ion ChromatographyD2036-09(15)(A).
Ion Selective Electrode4500-CN F-2021D2036-09(15)(A).
24. Cyanide—Available, mg/LCyanide Amenable to Chlorination (CATC); Manual distillation with MgCl 2 , followed by Titrimetric or Spectrophotometric4500-CN G-2021D2036-09(15)(B).
Flow injection and ligand exchange, followed by gas diffusion amperometry 594500-CN Q-2021D6888-16OIA-1677-09. 44
Automated Distillation and Colorimetry (no UV digestion)Kelada-01. 55
24A. Cyanide—Free, mg/LFlow Injection, followed by gas diffusion amperometry4500-CN R-2021D7237-18 (A)OIA-1677-09. 44
Manual micro-diffusion and colorimetryD4282-15.
25. Fluoride—Total, mg/LManual distillation, 6 followed by any of the following:4500-F B-2021D1179-16 (A).
Electrode, manual4500-F C-2021D1179-16 (B).
Electrode, automated4500-F G-2021I-4327-85. 2
Colorimetric, (SPADNS)4500-F D-2021.
Automated complexone4500-F E-2021.
Ion Chromatography300.0 Rev 2.1 (1993) and 300.1 Rev 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30. 3
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
26. Gold—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019.
AA furnace231.2 (Issued 1978) 13113 B-2020.
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPSee footnote. 34
27. Hardness—Total (as CaCO (3) , mg/LAutomated colorimetric130.1 (Issued 1971). 1
Titrimetric (EDTA)2340 C-2021D1126-17973.52B, 3 I-1338-85. 2
Ca plus Mg as their carbonates, by any approved method for Ca and Mg (See Parameters 13 and 33), provided that the sum of the lowest point of quantitation for Ca and Mg is below the NPDES permit requirement for Hardness.2340 B-2021.
28. Hydrogen ion (pH), pH unitsElectrometric measurement4500-H + B-2021D1293-18 (A or B)973.41, 3 I-1586-85. 2
Automated electrode150.2 (Dec. 1982) 1See footnote 21 I-2587-85. 2
29. Iridium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019.
AA furnace235.2 (Issued 1978). 1
ICP/MS3125 B-2020.
30. Iron—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D1068-15 (A)974.27, 3 I-3381-85. 2
AA furnace3113 B-2020D1068-15 (B).
STGFAA200.9, Rev. 2.2 (1994).
ICP/AES 36200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCP 36D4190-15See footnote. 34
Colorimetric (Phenanthroline)3500-Fe B-2011D1068-15 (C)See footnote. 22
31. Kjeldahl Nitrogen 5 —Total (as N), mg/LManual digestion 20 and distillation or gas diffusion, followed by any of the following:4500-N org B-2021 or C-2021 and 4500-NH 3 B-2021D3590-17 (A)I-4515-91. 45
Titration4500-NH 3 C-2021973.48. 3
NesslerizationD1426-15 (A).
Electrode4500-NH 3 D-2021 or E-2021D1426-15 (B).
Semi-automated phenate350.1 Rev. 2.0 (1993)4500-NH 3 G-2021 or 4500-NH 3 H-2021.
Manual phenate, salicylate, or other substituted phenols in Berthelot reaction based methods4500-NH 3 F-2021See footnote. 60
Automated gas diffusion, followed by conductivity cell analysisTimberline Ammonia-001. 74
Automated gas diffusion followed by fluorescence detector analysisFIAlab 100. 82
Automated Methods for TKN that do not require manual distillation.
Automated phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods colorimetric (auto digestion and distillation)351.1 (Rev. 1978) 1I-4551-78. 8
Semi-automated block digestor colorimetric (distillation not required)351.2 Rev. 2.0 (1993)4500-N org D-2021D3590-17 (B)I-4515-91. 45
Block digester, followed by Auto distillation and TitrationSee footnote. 39
Block digester, followed by Auto distillation and NesslerizationSee footnote. 40
Block Digester, followed by Flow injection gas diffusion (distillation not required)See footnote. 41
Digestion with peroxdisulfate, followed by Spectrophotometric (2,6-dimethyl phenol)Hach 10242. 76
Digestion with persulfate, followed by ColorimetricNCASI TNTP W10900. 77
32. Lead—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D3559-15 (A or B)974.27, 3 I-3399-85. 2
AA furnace3113 B-2020D3559-15 (D)I-4403-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Voltammetry 11D3559-15 (C).
Colorimetric (Dithizone)3500-Pb B-2020.
33. Magnesium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019D511-14 (B)974.27, 3 I-3447-85. 2
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPSee footnote. 34
Ion ChromatographyD6919-17.
34. Manganese—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D858-17 (A or B)974.27, 3 I-3454-85. 2
AA furnace3113 B-2020D858-17 (C).
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5, Rev. 4.2 (2003); 68 200.7, Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Colorimetric (Persulfate)3500-Mn B-2020920.203. 3
Colorimetric (Periodate)See footnote. 23
35. Mercury—Total, mg/LCold vapor, Manual245.1 Rev. 3.0 (1994)3112 B-2020D3223-17977.22, 3 I-3462-85. 2
Cold vapor, Automated245.2 (Issued 1974). 1
Cold vapor atomic fluorescence spectrometry (CVAFS)245.7 Rev. 2.0 (2005) 17I-4464-01. 71
Purge and Trap CVAFS1631E. 43
36. Molybdenum—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 D-2019I-3490-85. 2
AA furnace3113 B-2020I-3492-96. 47
ICP/AES200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCPSee footnote. 34
37. Nickel—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D1886-14 (A or B)I-3499-85. 2
AA furnace3113 B-2020D1886-14 (C)I-4503-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05, 70 I-4472-97. 81
DCP 36D4190-15See footnote. 34
38. Nitrate (as N), mg/LIon Chromatography300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30. 3
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
Ion Selective Electrode4500-NO 3 D-2019.
Colorimetric (Brucine sulfate)352.1 (Issued 1971) 1973.50, 3 419D, 86 p. 28. 9
Spectrophotometric (2,6-dimethylphenol)Hach 10206. 75
Nitrate-nitrite N minus Nitrite N (see parameters 39 and 40).
39. Nitrate-nitrite (as N), mg/LCadmium reduction, Manual4500-NO 3 E-2019D3867-16 (B).
Cadmium reduction, Automated353.2 Rev. 2.0 (1993)4500-NO 3 F-2019 or 4500-NO 3 I-2019D3867-16 (A)I-2545-90. 51
Automated hydrazine4500-NO 3 H-2019.
Reduction/ColorimetricSee footnote. 62
Ion Chromatography300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30. 3
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
Enzymatic reduction, followed by automated colorimetric determinationD7781-14I-2547-11, 72 I-2548-11, 72 N07-0003. 73
Enzymatic reduction, followed by manual colorimetric determination4500-NO 3 J-2018.
Spectrophotometric (2,6-dimethylphenol)Hach 10206. 75
40. Nitrite (as N), mg/LSpectrophotometric: Manual4500-NO 2 B-2021See footnote. 25
Automated (Diazotization)I-4540-85 2 see footnote, 62 I-2540-90. 80
Automated (*bypass cadmium reduction)353.2 Rev. 2.0 (1993)4500-NO 3 F-2019, 4500-NO 3 I-2019D3867-16 (A)I-4545-85. 2
Manual (*bypass cadmium or enzymatic reduction)4500-NO 3 E-2019, 4500-NO 3 J-2018D3867-16 (B).
Ion Chromatography300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30. 3
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
Automated (*bypass Enzymatic reduction)D7781-14I-2547-11, 72 I-2548-11, 72 N07-0003. 73
41. Oil and grease—Total recoverable, mg/LHexane extractable material (HEM): n -Hexane extraction and gravimetry1664 Rev. A 1664 Rev. B 425520 B or G-2021. 38
Silica gel treated HEM (SGT-HEM): Silica gel treatment and gravimetry1664 Rev. A, 1664 Rev. B 425520 B or G-2021 38 and 5520 F-2021. 38
42. Organic carbon—Total (TOC), mg/LCombustion5310 B-2014D7573-18a e1973.47, 3 p. 14. 24
Heated persulfate or UV persulfate oxidation5310 C-2014, 5310 D-2011D4839-03(17)973.47, 3, p. 14. 24
43. Organic nitrogen (as N), mg/LTotal Kjeldahl N (Parameter 31) minus ammonia N (Parameter 4).
44. Ortho-phosphate (as P), mg/LAscorbic acid method:
Automated365.1 Rev. 2.0 (1993)4500-P F-2021 or G-2021973.56, 3 I-4601-85, 2 I-2601-90. 80
Manual, single-reagent4500-P E-2021D515-88 (A)973.55. 3
Manual, two-reagent365.3 (Issued 1978). 1
Ion Chromatography300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30. 3
CIE/UV4140 B-2020D6508-15D6508, Rev. 2. 54
45. Osmium—Total 4 , mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 D-2019.
AA furnace252.2 (Issued 1978). 1
46. Oxygen, dissolved, mg/LWinkler (Azide modification)4500-O (B-F)-2021D888-18 (A)973.45B, 3 I-1575-78. 8
Electrode4500-O G-2021D888-18 (B)I-1576-78. 8
Luminescence-Based Sensor4500-O H-2021D888-18 (C)See footnotes.
47. Palladium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019.
AA furnace253.2 (Issued 1978). 1
ICP/MS3125 B-2020.
DCPSee footnote. 34
48. Phenols, mg/LManual distillation, 26 followed by any of the following:420.1 (Rev. 1978) 15530 B-2021D1783-01(12)
Colorimetric (4AAP) manual420.1 (Rev. 1978) 15530 D-2021 27D1783-01(12) (A or B).
Automated colorimetric (4AAP)420.4 Rev. 1.0 (1993).
49. Phosphorus (elemental), mg/LGas-liquid chromatographySee footnote. 28
50. Phosphorus—Total, mg/LDigestion, 20 followed by any of the following:4500-P B (5)-2021973.55. 3
Manual365.3 (Issued 1978) 14500-P E-2021D515-88 (A).
Automated ascorbic acid reduction365.1 Rev. 2.0 (1993)4500-P (F-H)-2021973.56, 3 I-4600-85. 2
ICP/AES 436200.7Rev. 4.4 (1994)3120 B-2020I-4471-97. 50
Semi-automated block digestor (TKP digestion)365.4 (Issued 1974) 1D515-88 (B)I-4610-91. 48
Digestion with persulfate, followed by ColorimetricNCASI TNTP W10900. 77
51. Platinum—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019.
AA furnace255.2 (Issued 1978). 1
ICP/MS3125 B-2020.
DCPSee footnote. 34
52. Potassium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019973.5, 3 I-3630-85. 2
ICP/AES200.7 Rev. 4.4 (1994)3120 B-2020.
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
Flame photometric3500-K B-2020.
Electrode3500-K C-2020.
Ion ChromatographyD6919-17.
53. Residue—Total, mg/LGravimetric, 103-105°2540 B-2020I-3750-85. 2
54. Residue—filterable, mg/LGravimetric, 180°2540 C-2020D5907-18 (B)I-1750-85. 2
55. Residue—non-filterable (TSS), mg/LGravimetric, 103-105° post-washing of residue2540 D-2020D5907-18 (A)I-3765-85. 2
56. Residue—settleable, mg/LVolumetric (Imhoff cone), or gravimetric2540 F-2020.
57. Residue—Volatile, mg/LGravimetric, 550°160.4 (Issued 1971) 12540 E-2020I-3753-85. 2
58. Rhodium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration, or3111 B-2019.
AA furnace265.2 (Issued 1978). 1
ICP/MS3125 B-2020.
59. Ruthenium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration, or3111 B-2019.
AA furnace267.2. 1
ICP/MS3125 B-2020.
60. Selenium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA furnace3113 B-2020D3859-15 (B)I-4668-98. 49
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES 36200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20.
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05 70 I-4472-97. 81
AA gaseous hydride3114 B-2020, or 3114 C-2020D3859-15 (A)I-3667-85. 2
61. Silica—Dissolved, 37 mg/L0.45-micron filtration followed by any of the following:
Colorimetric, Manual4500-SiO 2 C-2021D859-16I-1700-85. 2
Automated (Molybdosilicate)4500-SiO 2 E-2021 or F-2021I-2700-85. 2
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
62. Silver—Total, 4 31 mg/LDigestion, 4 29 followed by any of the following:
AA direct aspiration3111 B-2019 or 3111 C-2019974.27, 3 p. 37, 9 I-3720-85. 2
AA furnace3113 B-2020I-4724-89. 51
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4472-97. 81
DCPSee footnote. 34
63. Sodium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019973.54, 3 I-3735-85. 2
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPSee footnote. 34
Flame photometric3500-Na B-2020.
Ion ChromatographyD6919-17.
64. Specific conductance, micromhos/cm at 25 °CWheatstone bridge120.1 (Rev. 1982) 12510 B-2021D1125-95(99) (A)973.40, 3 I-2781-85. 2
65. Sulfate (as SO 4), mg/LAutomated colorimetric375.2 Rev. 2.0 (1993)4500-SO 42 F-2021 or G-2021.
Gravimetric4500-SO 42 C-2021 or D-2021925.54. 3
Turbidimetric4500-SO 42 E-2021D516-16.
Ion Chromatography300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997)4110 B-2020 or C-2020D4327-17993.30, 3 I-4020-05. 70
CIE/UV4140 B-2020D6508-15D6508 Rev. 2. 54
66. Sulfide (as S), mg/LSample Pretreatment4500-S 2 B, C-2021.
Titrimetric (iodine)4500-S 2 F-2021I-3840-85. 2
Colorimetric (methylene blue)4500-S 2 D-2021.
Ion Selective Electrode4500-S 2 G-2021D4658-15.
67. Sulfite (as SO 3), mg/LTitrimetric (iodine-iodate)4500-SO 32 B-2021.
68. Surfactants, mg/LColorimetric (methylene blue)5540 C-2021D2330-20.
69. Temperature, °CThermometric2550 B-2010See footnote. 32
70. Thallium-Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019.
AA furnace279.2 (Issued 1978) 13113 B-2020.
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES200.7 Rev. 4.4 (1994)3120 B-2020D1976-20.
ICP/MS200.8, Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4471-97 50 I-4472-97. 81
71. Tin—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 B-2019I-3850-78. 8
AA furnace3113 B-2020.
STGFAA200.9 Rev. 2.2 (1994).
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994).
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
72. Titanium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 D-2019.
AA furnace283.2 (Issued 1978). 1
ICP/AES200.7 Rev. 4.4 (1994).
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14. 3
DCPSee footnote. 34
73. Turbidity, NTU 53Nephelometric180.1, Rev. 2.0 (1993)2130 B-2020D1889-00I-3860-85, 2 see footnotes. 656667
74. Vanadium—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration3111 D-2019.
AA furnace3113 B-2020D3373-17.
ICP/AES200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05. 70
DCPD4190-15See footnote. 34
Colorimetric (Gallic Acid)3500-V B-2011.
75. Zinc—Total, 4 mg/LDigestion, 4 followed by any of the following:
AA direct aspiration 363111 B-2019 or 3111 C-2019D1691-17 (A or B)974.27 3 p. 37, 9 I-3900-85. 2
AA furnace289.2 (Issued 1978). 1
ICP/AES 36200.5 Rev. 4.2 (2003), 68 200.7, Rev. 4.4 (1994)3120 B-2020D1976-20I-4471-97. 50
ICP/MS200.8 Rev. 5.4 (1994)3125 B-2020D5673-16993.14, 3 I-4020-05, 70 I-4472-97. 81
DCP 36D4190-15See footnote. 34
Colorimetric (Zincon)3500 Zn B-2020See footnote. 33
76. Acid Mine Drainage1627. 69

Table IC—List of Approved Test Procedures for Non-Pesticide Organic Compounds
Parameter1MethodEPA2 7Standard methods17ASTMOther
1. AcenaphtheneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
2. AcenaphthyleneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
3. AcroleinGC603
GC/MS624.1 4 , 1624B.
4. AcrylonitrileGC603
GC/MS624.1 4 , 1624BO-4127-96. 13
5. AnthraceneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
6. BenzeneGC6026200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
7. BenzidineSpectro-photometricSee footnote 3 p.1.
GC/MS625.1 5 , 1625B6410 B-2020.
HPLC605
8. Benzo(a)anthraceneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
9. Benzo(a)pyreneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
10. Benzo(b)fluorantheneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
11. Benzo(g,h,i)peryleneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
12. Benzo(k)fluorantheneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
13. Benzyl chlorideGCSee footnote 3 p. 130.
GC/MSSee footnote 6 p. S102.
14. Butyl benzyl phthalateGC606
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
15. bis(2-Chloroethoxy) methaneGC611
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
16. bis(2-Chloroethyl) etherGC611
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
17. bis(2-Ethylhexyl) phthalateGC606
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
18. BromodichloromethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
19. BromoformGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
20. BromomethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
21. 4-Bromophenyl phenyl etherGC611
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
22. Carbon tetrachlorideGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
23. 4-Chloro-3-methyl phenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
24. ChlorobenzeneGC601, 6026200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 O-4436-16. 14
25. ChloroethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96. 13
26. 2-Chloroethylvinyl etherGC601
GC/MS624.1, 1624B.
27. ChloroformGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
28. ChloromethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
29. 2-ChloronaphthaleneGC612
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
30. 2-ChlorophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
31. 4-Chlorophenyl phenyl etherGC611
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
32. ChryseneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
33. Dibenzo(a,h)anthraceneGC610
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
34. DibromochloromethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
35. 1,2-DichlorobenzeneGC601, 6026200 C-2020.
GC/MS624.1, 1625B6200 B-2020See footnote 9 p. 27, O-4127-96 13 , O-4436-16. 14
36. 1,3-DichlorobenzeneGC601, 6026200 C-2020.
GC/MS624.1, 1625B6200 B-2020See footnote 9 p. 27, O-4127-96. 13
37. 1,4-DichlorobenzeneGC601, 6026200 C-2020.
GC/MS624.1, 1625B6200 B-2020See footnote 9 p. 27, O-4127-96 13 , O-4436-16. 14
38. 3,3′-DichlorobenzidineGC/MS625.1, 1625B6410 B-2020.
HPLC605.
39. DichlorodifluoromethaneGC601.
GC/MS6200 B-2020O-4127-96 13 , O-4436-16. 14
40. 1,1-DichloroethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
41. 1,2-DichloroethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
42. 1,1-DichloroetheneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
43. trans -1,2-DichloroetheneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
44. 2,4-DichlorophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
45. 1,2-DichloropropaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 O-4436-16. 14
46. cis -1,3-DichloropropeneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
47. trans -1,3-DichloropropeneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
48. Diethyl phthalateGC606.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
49. 2,4-DimethylphenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
50. Dimethyl phthalateGC606.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
51. Di- n -butyl phthalateGC606.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
52. Di- n -octyl phthalateGC606.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
53. 2, 4-DinitrophenolGC6046420 B-2021See footnote 9 p. 27.
GC/MS625.1, 1625B6410 B-2020.
54. 2,4-DinitrotolueneGC609.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
55. 2,6-DinitrotolueneGC609.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
56. EpichlorohydrinGCSee footnote 3 p. 130.
GC/MSSee footnote 6 p. S102.
57. EthylbenzeneGC6026200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
58. FluorantheneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
59. FluoreneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
60. 1,2,3,4,6,7,8-Heptachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
61. 1,2,3,4,7,8,9-Heptachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
62. 1,2,3,4,6,7,8- Heptachloro-dibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
63. HexachlorobenzeneGC612.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
64. HexachlorobutadieneGC612.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27, O-4127-96. 13
65. HexachlorocyclopentadieneGC612.
GC/MS625.1 5 , 1625B6410 B-2020See footnote 9 , p. 27, O-4127-96. 13
66. 1,2,3,4,7,8-Hexachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
67. 1,2,3,6,7,8-Hexachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
68. 1,2,3,7,8,9-Hexachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
69. 2,3,4,6,7,8-Hexachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
70. 1,2,3,4,7,8-Hexachloro-dibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
71. 1,2,3,6,7,8-Hexachloro-dibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
72. 1,2,3,7,8,9-Hexachloro-dibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
73. HexachloroethaneGC612.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27, O-4127-96. 13
74. Indeno(1,2,3-c,d) pyreneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
75. IsophoroneGC609.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
76. Methylene chlorideGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
77. 2-Methyl-4,6-dinitrophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
78. NaphthaleneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021.
79. NitrobenzeneGC609.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLCD4657-92 (98).
80. 2-NitrophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
81. 4-NitrophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
82. N-NitrosodimethylamineGC607.
GC/MS625.1 5 , 1625B6410 B-2020See footnote 9 p. 27.
83. N-Nitrosodi- n -propylamineGC607.
GC/MS625.1 5 , 1625B6410 B-2020See footnote 9 p. 27.
84. N-NitrosodiphenylamineGC607.
GC/MS625.1 5 , 1625B6410 B-2020See footnote 9 p. 27.
85. OctachlorodibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
86. Octachlorodibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
87. 2,2′-oxybis(1-chloropropane) 12 [also known as bis(2-Chloro-1-methylethyl) ether]GC611.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
88. PCB-1016GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
89. PCB-1221GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
90. PCB-1232GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
91. PCB-1242GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
92. PCB-1248GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
93. PCB-1254GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
94. PCB-1260GC608.3See footnote 3 p. 43, see footnote. 8
GC/MS625.16410 B-2020.
95. 1,2,3,7,8-Pentachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
96. 2,3,4,7,8-Pentachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
97. 1,2,3,7,8-Pentachloro-dibenzo- p -dioxinGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
98. PentachlorophenolGC6046420 B-2021See footnote 3 p. 140.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
99. PhenanthreneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
100. PhenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
101. PyreneGC610.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
HPLC6106440 B-2021D4657-92 (98).
102. 2,3,7,8-Tetrachloro-dibenzofuranGC/MS1613B 10SGS AXYS 16130 15 , PAM 16130-SSI. 16
103. 2,3,7,8-Tetrachloro-dibenzo- p -dioxinGC/MS613, 625.1 5 , 1613BSGS AXYS 16130 15 , PAM 16130-SSI. 16
104. 1,1,2,2-TetrachloroethaneGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96. 13
105. TetrachloroetheneGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
106. TolueneGC6026200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
107. 1,2,4-TrichlorobenzeneGC612See footnote 3 p. 130.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27, O-4127-96 13 , O-4436-16. 14
108. 1,1,1-TrichloroethaneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
109. 1,1,2-TrichloroethaneGC6016200 C-2020See footnote 3 p. 130.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
110. TrichloroetheneGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
111. TrichlorofluoromethaneGC6016200 C-2020.
GC/MS624.16200 B-2020O-4127-96. 13
112. 2,4,6-TrichlorophenolGC6046420 B-2021.
GC/MS625.1, 1625B6410 B-2020See footnote 9 p. 27.
113. Vinyl chlorideGC6016200 C-2020.
GC/MS624.1, 1624B6200 B-2020O-4127-96 13 , O-4436-16. 14
114. NonylphenolGC/MSD7065-17.
115. Bisphenol A (BPA)GC/MSD7065-17.
116. p-tert -Octylphenol (OP)GC/MSD7065-17.
117. Nonylphenol Monoethoxylate (NP1EO)GC/MSD7065-17.
118. Nonylphenol Diethoxylate (NP2EO)GC/MSD7065-17.
119. Adsorbable Organic Halides (AOX)Adsorption and Coulometric Titration1650. 11
120. Chlorinated PhenolicsIn Situ Acetylation and GC/MS1653. 11
Table IC notes:

1 All parameters are expressed in micrograms per liter (µg/L) except for Method 1613B, in which the parameters are expressed in picograms per liter (pg/L).
2 The full text of Methods 601-613, 1613B, 1624B, and 1625B are provided at appendix A, Test Procedures for Analysis of Organic Pollutants. The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at appendix B of this part, Definition and Procedure for the Determination of the Method Detection Limit. These methods are available at: https://www.epa.gov/cwa-methods as individual PDF files.
3 Methods for Benzidine: Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA.
4 Method 624.1 may be used for quantitative determination of acrolein and acrylonitrile, provided that the laboratory has documentation to substantiate the ability to detect and quantify these analytes at levels necessary to comply with any associated regulations. In addition, the use of sample introduction techniques other than simple purge-and-trap may be required. QC acceptance criteria from Method 603 should be used when analyzing samples for acrolein and acrylonitrile in the absence of such criteria in Method 624.1.
5 Method 625.1 may be extended to include benzidine, hexachlorocyclopentadiene, N-nitrosodimethylamine, N-nitrosodi- n -propylamine, and N-nitrosodiphenylamine. However, when they are known to be present, Methods 605, 607, and 612, or Method 1625B, are preferred methods for these compounds. Method 625.1 may be applied to 2,3,7,8-Tetrachloro-dibenzo-p -dioxin for screening purposes only.
6 Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).
7 Each analyst must make an initial, one-time demonstration of their ability to generate acceptable precision and accuracy with Methods 601-603, 1624B, and 1625B in accordance with procedures in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis must spike and analyze 10% (5% for Methods 624.1 and 625.1 and 100% for methods 1624B and 1625B) of all samples to monitor and evaluate laboratory data quality in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the quality control (QC) acceptance criteria in the pertinent method, analytical results for that parameter in the unspiked sample are suspect. The results should be reported but cannot be used to demonstrate regulatory compliance. If the method does not contain QC acceptance criteria, control limits of ±three standard deviations around the mean of a minimum of five replicate measurements must be used. These quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.
8 Organochlorine Pesticides and PCBs in Wastewater Using EmporeTM Disk. Revised October 28, 1994. 3M Corporation.
9 Method O-3116-87 is in Open File Report 93-125, Methods of Analysis by U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. USGS.
10 Analysts may use Fluid Management Systems, Inc. Power-Prep system in place of manual cleanup provided the analyst meets the requirements of Method 1613B (as specified in Section 9 of the method) and permitting authorities. Method 1613, Revision B, Tetra- through Octa-Chlorinated Dioxins and Furans by Isotope Dilution HRGC/HRMS. Revision B, 1994. U.S. EPA. The full text of this method is provided in appendix A to this part and at https://www.epa.gov/cwa-methods/approved-cwa-test-methods-organic-compounds.
11 Method 1650, Adsorbable Organic Halides by Adsorption and Coulometric Titration. Revision C, 1997 U.S. EPA. Method 1653, Chlorinated Phenolics in Wastewater by In Situ Acetylation and GCMS. Revision A, 1997 U.S. EPA. The full text for both of these methods is provided at appendix A in part 430 of this chapter, The Pulp, Paper, and Paperboard Point Source Category.
12 The compound was formerly inaccurately labeled as 2,2′-oxybis(2-chloropropane) and bis(2-chloroisopropyl) ether. Some versions of Methods 611, and 1625 inaccurately list the analyte as “bis(2-chloroisopropyl) ether,” but use the correct CAS number of 108-60-1.
13 Method O-4127-96, U.S. Geological Survey Open-File Report 97-829, Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of 86 volatile organic compounds in water by gas chromatography/mass spectrometry, including detections less than reporting limits,1998, USGS.
14 Method O-4436-16 U.S. Geological Survey Techniques and Methods, book 5, chap. B12, Determination of heat purgeable and ambient purgeable volatile organic compounds in water by gas chromatography/mass spectrometry, 2016, USGS.
15 SGS AXYS Method 16130, “Determination of 2,3,7,8-Substituted Tetra- through Octa-Chlorinated Dibenzo-p -Dioxins and Dibenzofurans (CDDs/CDFs) Using Waters and Agilent Gas Chromatography-Tandem-Mass Spectrometry (GC/MS/MS), Revision 1.0” is available at: https://www.sgsaxys.com/wp-content/uploads/2022/09/SGS-AXYS-Method-16130-Rev-1.0.pdf.
16 Pace Analytical Method PAM-16130-SSI, “Determination of 2,3,7,8-Substituted Tetra- through Octa-Chlorinated Dibenzo-p -Dioxins and Dibenzofurans (CDDs/CDFs) Using Shimadzu Gas Chromatography Mass Spectrometry (GC-MS/MS), Revision 1.1,” is available at: pacelabs.com.
17 Please refer to the following applicable Quality Control Section: Part 6000 Individual Organic Compounds, 6020 (2019). The Quality Control Standards are available for download at standardmethods.org at no charge.
Table ID—List of Approved Test Procedures for Pesticides1
ParameterMethodEPAStandard methods 15ASTMOther
1. AldrinGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96 (02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020.
2. AmetrynGC507, 619See footnote 3 p. 83, see footnote 9 O-3106-93, see footnote 6 p. S68.
GC/MS525.2, 625.1See footnote 14 O-1121-91.
3. AminocarbTLCSee footnote 3 p. 94, see footnote 6 p. S60.
HPLC632.
4. AtratonGC619See footnote 3 p. 83, see footnote 6 p. S68.
GC/MS625.1.
5. AtrazineGC507, 619, 608.3See footnote 3 p. 83, see footnote 6 p. S68, see footnote 9 O-3106-93.
HPLC/MSSee footnote 12 O-2060-01.
GC/MS525.1, 525.2, 625.1See footnote 11 O-1126-95.
6. Azinphos methylGC614, 622, 1657See footnote 3 p. 25, see footnote 6 p. S51.
GC-MS625.1See footnote 11 O-1126-95.
7. BarbanTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
GC/MS625.1.
8. α-BHCGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 8 3M0222.
GC/MS625.1 56410 B-2020See footnote 11 O-1126-95.
9. β-BHCGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 8 3M0222.
GC/MS625.16410 B-2020.
10. δ-BHCGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 8 3M0222.
GC/MS625.16410 B-2020.
11. γ-BHC (Lindane)GC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 , O-3104-83, see footnote 8 3M0222.
GC/MS625.1 56410 B-2020See footnote 11 , O-1126-95.
12. CaptanGC617, 608.36630 B-2021D3086-90, D5812-96(02)See footnote 3 p. 7.
13. CarbarylTLCSee footnote 3 p. 94, see footnote 6 p. S60.
HPLC531.1, 632.
HPLC/MS553See footnote 12 O-2060-01.
GC/MS625.1See footnote 11 O-1126-95.
14. CarbophenothionGC617, 608.36630 B-2021See footnote 4 page 27, see footnote 6 p. S73.
GC/MS625.1.
15. ChlordaneGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020.
16. ChloroprophamTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
GC/MS625.1.
17. 2,4-DGC6156640 B-2021See footnote 3 p. 115, see footnote 4 O-3105-83.
HPLC/MSSee footnote 12 O-2060-01.
18. 4,4′-DDDGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3105-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020.
19. 4,4′-DDEGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 , O-3104-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020See footnote 11 O-1126-95.
20. 4,4′-DDTGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020.
21. Demeton-OGC614, 622See footnote 3 p. 25, see footnote 6 p. S51.
GC/MS625.1
22. Demeton-S.GC614, 622See footnote 3 p. 25, see footnote 6 p. S51.
GC/MS625.1.
23. DiazinonGC507, 614, 622, 1657See footnote 3 p. 25, see footnote 4 O-3104-83, see footnote 6 p. S51.
GC/MS525.2, 625.1See footnote 11 O-1126-95.
24. DicambaGC615See footnote 3 p. 115.
HPLC/MSSee footnote 12 O-2060-01.
25. DichlofenthionGC622.1See footnote 4 page 27, see footnote 6 p. S73.
26. DichloranGC608.2, 617, 608.36630 B-2021See footnote 3 p. 7.
27. DicofolGC617, 608.3See footnote 4 O-3104-83.
28. DieldrinGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS625.16410 B-2020See footnote 11 O-1126-95.
29. DioxathionGC614.1, 1657See footnote 4 page 27, see footnote 6 p. S73.
30. DisulfotonGC507, 614, 622, 1657See footnote 3 p. 25, see footnote 6 p. S51.
GC/MS525.2, 625.1See footnote 11 O-1126-95.
31. DiuronTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
HPLC/MS553See footnote 12 O-2060-01.
32. Endosulfan IGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS625.1 56410 B-2020See footnote 13 O-2002-01.
33. Endosulfan IIGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 8 3M0222.
GC/MS625.1 56410 B-2020See footnote 13 O-2002-01.
34. Endosulfan SulfateGC617, 608.36630 C-2021See footnote 8 3M0222.
GC/MS625.16410 B-2020.
35. EndrinGC505, 508, 617, 1656, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS525.1, 525.2, 625.1 56410 B-2020.
36. Endrin aldehydeGC617, 608.36630 C-2021See footnote 8 3M0222.
GC/MS625.16410 B-2020.
37. EthionGC614, 614.1, 1657See footnote 4 page 27, see footnote 6 , p. S73.
GC/MS625.1See footnote 13 O-2002-01.
38. FenuronTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
HPLC/MSSee footnote 12 O-2060-01.
39. Fenuron-TCATLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
40. HeptachlorGC505, 508, 617, 1656, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS525.1, 525.2, 625.16410 B-2020.
41. Heptachlor epoxideGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 6 p. S73, see footnote 8 3M0222.
GC/MS625.16410 B-2020.
42. IsodrinGC617, 608.36630 B-2021 & C-2021See footnote 4 O-3104-83, see footnote 6 p. S73.
GC/MS625.1.
43. LinuronGCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
HPLC/MS553See footnote 12 O-2060-01.
GC/MSSee footnote 11 O-1126-95.
44. MalathionGC614, 16576630 B-2021See footnote 3 p. 25, see footnote 6 p. S51.
GC/MS625.1See footnote 11 O-1126-95.
45. MethiocarbTLCSee footnote 3 p. 94, see footnote 6 p. S60.
HPLC632.
HPLC/MSSee footnote 12 O-2060-01.
46. MethoxychlorGC505, 508, 608.2, 617, 1656, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83, see footnote 8 3M0222.
GC/MS525.1, 525.2, 625.1See footnote 11 O-1126-95.
47. MexacarbateTLCSee footnote 3 p. 94, see footnote 6 p. S60.
HPLC632.
GC/MS625.1.
48. MirexGC617, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 4 O-3104-83.
GC/MS625.1.
49. MonuronTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
50. Monuron-TCATLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
51. NeburonTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
HPLC/MSSee footnote 12 O-2060-01.
52. Parathion methylGC614, 622, 16576630 B-2021See footnote 4 page 27, see footnote 3 p. 25.
GC/MS625.1See footnote 11 O-1126-95.
53. Parathion ethylGC6146630 B-2021See footnote 4 page 27, see footnote 3 p. 25.
GC/MSSee footnote 11 O-1126-95.
54. PCNBGC608.1, 617, 608.36630 B-2021 & C-2021D3086-90 , D5812-96(02)See footnote 3 p. 7.
55. PerthaneGC617, 608.3D3086-90, D5812-96(02)See footnote 4 O-3104-83.
56. PrometonGC507, 619See footnote 3 p. 83, see footnote 6 p. S68, see footnote 9 O-3106-93.
GC/MS525.2, 625.1See footnote 11 O-1126-95.
57. PrometrynGC507, 619See footnote 3 p. 83, see footnote 6 p. S68, see footnote 9 O-3106-93.
GC/MS525.1, 525.2, 625.1See footnote 13 O-2002-01.
58. PropazineGC507, 619, 1656, 608.3See footnote 3 p. 83, see footnote 6 p. S68, see footnote 9 O-3106-93.
GC/MS525.1, 525.2, 625.1
59. ProphamTLCSee footnote 3 p. 10, see footnote 6 p. S64.
HPLC632.
HPLC/MSSee footnote 12 O-2060-01.
60. PropoxurTLCSee footnote 3 p. 94, see footnote 6 , p. S60.
HPLC632.
61. SecbumetonTLCSee footnote 3 p. 83, see footnote 6 p. S68.
GC619.
62. SiduronTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
HPLC/MSSee footnote 12 O-2060-01.
63. SimazineGC505, 507, 619, 1656, 608.3See footnote 3 p. 83, see footnote 6 p. S68, see footnote 9 O-3106-93.
GC/MS525.1, 525.2, 625.1See footnote 11 O-1126-95.
64. StrobaneGC617, 608.36630 B-2021 & C-2021See footnote 3 p. 7.
65. SwepTLCSee footnote 3 p. 104, see footnote 6 p. S64.
HPLC632.
66. 2,4,5-TGC6156640 B-2021See footnote 3 p. 115, see footnote 4 O-3105-83.
67. 2,4,5-TP (Silvex)GC6156640 B-2021See footnote 3 p. 115, see footnote 4 O-3105-83.
68. TerbuthylazineGC619, 1656, 608.3See footnote 3 p. 83, see footnote 6 p. S68.
GC/MSSee footnote 13 O-2002-01.
69. ToxapheneGC505, 508, 617, 1656, 608.36630 B-2021 & C-2021D3086-90, D5812-96(02)See footnote 3 p. 7, see footnote 8 , see footnote 4 O-3105-83.
GC/MS525.1, 525.2, 625.16410 B-2020.
70. TrifluralinGC508, 617, 627, 1656, 608.36630 B-2021See footnote 3 p. 7, see footnote 9 O-3106-93.
GC/MS525.2, 625.1See footnote 11 O-1126-95.

Table ID notes:
1 Pesticides are listed in this table by common name for the convenience of the reader. Additional pesticides may be found under table IC of this section, where entries are listed by chemical name.
2 The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at appendix B to this part, Definition and Procedure for the Determination of the Method Detection Limit.
3 Methods for Benzidine, Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA. This EPA publication includes thin-layer chromatography (TLC) methods.
4 Methods for the Determination of Organic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A3. 1987. USGS.
5 The method may be extended to include α-BHC, γ-BHC, endosulfan I, endosulfan II, and endrin. However, when they are known to exist, Method 608 is the preferred method.
6 Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).
7 Each analyst must make an initial, one-time, demonstration of their ability to generate acceptable precision and accuracy with Methods 608.3 and 625.1 in accordance with procedures given in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis, must spike and analyze 10% of all samples analyzed with Method 608.3 or 5% of all samples analyzed with Method 625.1 to monitor and evaluate laboratory data quality in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the warning limits, the analytical results for that parameter in the unspiked sample are suspect. The results should be reported, but cannot be used to demonstrate regulatory compliance. These quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.
8 Organochlorine Pesticides and PCBs in Wastewater Using EmporeTM Disk. Revised October 28, 1994. 3M Corporation.
9 Method O-3106-93 is in Open File Report 94-37, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Triazine and Other Nitrogen-Containing Compounds by Gas Chromatography with Nitrogen Phosphorus Detectors. 1994. USGS.
10 EPA Methods 608.1, 608.2, 614, 614.1, 615, 617, 619, 622, 622.1, 627, and 632 are found in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, EPA 821-R-92-002, April 1992, U.S. EPA. EPA Methods 505, 507, 508, 525.1, 531.1 and 553 are in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume II, EPA 821-R-93-010B, 1993, U.S. EPA. EPA Method 525.2 is in Determination of Organic Compounds in Drinking Water by Liquid-Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry, Revision 2.0, 1995, U.S. EPA. EPA methods 1656 and 1657 are in Methods for The Determination of Nonconventional Pesticides In Municipal and Industrial Wastewater, Volume I, EPA 821-R-93-010A, 1993, U.S. EPA. Methods 608.3 and 625.1 are available at: cwa-methods/approved-cwa-test-methods-organic-compounds.
11 Method O-1126-95 is in Open-File Report 95-181, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion monitoring. 1995. USGS.
12 Method O-2060-01 is in Water-Resources Investigations Report 01-4134, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by Graphitized Carbon-Based Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass Spectrometry. 2001. USGS.
13 Method O-2002-01 is in Water-Resources Investigations Report 01-4098, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of moderate-use pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass spectrometry. 2001. USGS.
14 Method O-1121-91 is in Open-File Report 91-519, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of organonitrogen herbicides in water by solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion monitoring. 1992. USGS.
15 Please refer to the following applicable Quality Control Section: Part 6000 Methods, Individual Organic Compounds 6020 (2019). These Quality Control Standards are available for download at www.standardmethods.org at no charge.

* * * * *

Table IH—List of Approved Microbiological Methods for Ambient Water
Parameter and unitsMethod 1EPAStandard methodsAOAC, ASTM, USGSOther
Bacteria
1. Coliform (fecal), number per 100 mLMost Probable Number (MPN), 5 tube, 3 dilution, orp. 132 39221 E-2014, 9221 F-2014. 32
Membrane filter (MF) 2 , single stepp. 124 39222 D-2015 26B-0050-85. 4
2. Coliform (total), number per 100 mLMPN, 5 tube, 3 dilution, orp. 114 39221 B-2014.
MF 2 , single step orp. 108 39222 B-2015 27B-0025-85. 4
MF 2 , two step with enrichmentp. 111 39222 B-2015. 27
3. E. coli, number per 100 mLMPN 5713 , multiple tube, or9221 B.3-2014/9221 F-2014. 101232
Multiple tube/multiple well, or9223 B-2016 11991.15 9Colilert® 1115 , Colilert-18®. 111415
MF 2567 , two step, or1103.2 189222 B-2015/9222 I-2015 17 , 9213 D-2007D5392-93. 8
Single step1603.1 19 , 1604 20m-ColiBlue24® 16 , KwikCount EC. 2829
4. Fecal streptococci, number per 100 mLMPN, 5 tube, 3 dilution, orp. 139 39230 B-2013.
MF 2 , orp. 136 39230 C-2013 30B-0055-85. 4
Plate countp. 143. 3
5. Enterococci, number per 100 mLMPN 57 , multiple tube/multiple well, or9230 D-2013D6503-99 8Enterolert®. 1121
MF 2567 two step, or1106.2 229230 C-2013 30D5259-92. 8
Single step, or1600.1 239230 C-2013. 30
Plate countp. 143. 3
Protozoa
6. CryptosporidiumFiltration/IMS/FA1622 24 , 1623 25 , 1623.1. 2531
7. GiardiaFiltration/IMS/FA1623 25 , 1623.1. 2531
Table 1H notes:

1 The method must be specified when results are reported.
2 A 0.45-µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of extractables which could interfere with their growth.
3 Microbiological Methods for Monitoring the Environment, Water and Wastes. EPA/600/8-78/017. 1978. US EPA.
4 U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS.
5 Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample.
6 When the MF method has not been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and comparability of results.
7 To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines.
8 Annual Book of ASTM Standards—Water and Environmental Technology. Section 11.02. 2000, 1999, 1996. ASTM International.
9 Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. 1995. AOAC International.
10 The multiple-tube fermentation test is used in 9221B.3-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis.
11 These tests are collectively known as defined enzyme substrate tests.
12 After prior enrichment in a presumptive medium for total coliform using 9221B.3-2014, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 µg/mL of MUG may be used.
13 Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert® may be enumerated with the multiple-well procedures, Quanti-Tray® or Quanti-Tray®/2000, and the MPN calculated from the table provided by the manufacturer.
14 Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35 °C, rather than the 24 h required for the Colilert® test and is recommended for marine water samples.
15 Descriptions of the Colilert®, Colilert-18®, Quanti-Tray ®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories Inc.
16 A description of the mColiBlue24® test may be obtained from Hach Company.
17 Subject coliform positive samples determined by 9222B-2015 or other membrane filter procedure to 9222I-2015 using NA-MUG media.
18 Method 1103.2: Escherichia coli ( E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC), EPA-821-R-23-009. September 2023. US EPA.
19 Method 1603.1: Escherichia coli ( E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-23-008. September 2023 . US EPA.
20 Method 1604: Total Coliforms and Escherichia coli ( E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium), EPA 821-R-02-024. September 2002. US EPA.
21 A description of the Enterolert® test may be obtained from IDEXX Laboratories Inc.
22 Method 1106.2: Enterococci in Water by Membrane Filtration Using membrane- Enterococcus -Esculin Iron Agar (mE-EIA), EPA-821-R-23-007. September 2023. US EPA.
23 Method 1600.1: Enterococci in Water by Membrane Filtration Using membrane- Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-21-006. September 2023. US EPA.
24 Method 1622 uses a filtration, concentration, immunomagnetic separation of oocysts from captured material, immunofluorescence assay to determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the detection of Cryptosporidium. Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, EPA-821-R-05-001. December 2005. US EPA.
25 Methods 1623 and 1623.1 use a filtration, concentration, immunomagnetic separation of oocysts and cysts from captured material, immunofluorescence assay to determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the simultaneous detection of Cryptosporidium and Giardia oocysts and cysts. Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA-821-R-05-002. December 2005. US EPA. Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA 816-R-12-001. January 2012. US EPA.
26 On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources.
27 On a monthly basis, at least ten sheen colonies from positive samples must be verified using Lauryl Tryptose Broth and brilliant green lactose bile broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources.
28 A description of KwikCount EC may be obtained from Roth Bioscience, LLC.
29 Approved for the analyses of E. coli in freshwater only.
30 Verification of colonies by incubation of BHI agar at 10 ± 0.5 °C for 48 ± 3 h is optional. As per the Errata to the 23rd Edition of Standard Methods for the Examination of Water and Wastewater “Growth on a BHI agar plate incubated at 10 ± 0.5 °C for 48 ± 3 h is further verification that the colony belongs to the genus Enterococcus.”
31 Method 1623.1 includes updated acceptance criteria for IPR, OPR, and MS/MSD and clarifications and revisions based on the use of Method 1623 for years and technical support questions.
32 9221 F.2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted vials collect gas produced by thermotolerant fecal coliforms.

* * * *

(b) The material listed in this paragraph (b) is incorporated by reference into this section with the approval of the Director of the Federal Register under 5 U.S.C. 552(a) and 1 CFR part 51. All approved incorporation by reference (IBR) material is available for inspection at the EPA and at the National Archives and Records Administration (NARA). Contact the EPA at: EPA's Water Docket, EPA West, 1301 Constitution Avenue NW, Room 3334, Washington, DC 20004; telephone: 202-566-2426; email: docket-customerservice@epa.gov. For information on the availability of this material at NARA, visit www.archives.gov/federal-register/cfr/ibr-locations or email fr.inspection@nara.gov. The material may be obtained from the following sources in this paragraph (b).

(1) Environmental Monitoring and Support Laboratory, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available athttp://water.epa.gov/scitech/methods/cwa/index.cfm or from: National Technical Information Service, 5285 Port Royal Road, Springfield, Virginia 22161

(i) Microbiological Methods for Monitoring the Environment, Water, and Wastes. 1978. EPA/600/8-78/017, Pub. No. PB-290329/A.S.

(A) Part III Analytical Methodology, Section B Total Coliform Methods, page 108. Table IA, Note 3; Table IH, Note 3.

(B) Part III Analytical Methodology, Section B Total Coliform Methods, 2.6.2 Two-Step Enrichment Procedure, page 111. Table IA, Note 3; Table IH, Note 3.

(C) Part III Analytical Methodology, Section B Total Coliform Methods, 4 Most Probable Number (MPN) Method, page 114. Table IA, Note 3; Table IH, Note 3.

(D) Part III Analytical Methodology, Section C Fecal Coliform Methods, 2 Direct Membrane Filter (MF) Method, page 124. Table IA, Note 3; Table IH, Note 3.

(E) Part III, Analytical Methodology, Section C Fecal Coliform Methods, 5 Most Probable Number (MPN) Method, page 132. Table IA, Note 3; Table IH, Note 3.

(F) Part III Analytical Methodology, Section D Fecal Streptococci, 2 Membrane Filter (MF) Method, page 136. Table IA, Note 3; Table IH, Note 3.

(G) Part III Analytical Methodology, Section D Fecal Streptococci, 4 Most Probable Number Method, page 139. Table IA, Note 3; Table IH, Note 3.

(H) Part III Analytical Methodology, Section D Fecal Streptococci, 5 Pour Plate Method, page 143. Table IA, Note 3; Table IH, Note 3.

(ii) [Reserved]

(2) Environmental Monitoring and Support Laboratory, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available athttp://water.epa.gov/scitech/methods/cwa/index.cfm.

(3) National Exposure Risk Laboratory-Cincinnati, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available from http://water.epa.gov/scitech/methods/cwa/index.cfm or from the National Technical Information Service (NTIS), 5285 Port Royal Road, Springfield, VA 22161. Telephone: 800-553-6847.

(i) Methods for the Determination of Inorganic Substances in Environmental Samples. August 1993. EPA/600/R-93/100, Pub. No. PB 94120821. Table IB, Note 52.

(A) Method 180.1, Determination of Turbidity by Nephelometry. Revision 2.0. Table IB, Note 52.

(B) Method 300.0, Determination of Inorganic Anions by Ion Chromatography. Revision 2.1. Table IB, Note 52.

(C) Method 335.4, Determination of Total Cyanide by Semi-Automated Colorimetry. Revision 1.0. Table IB, Notes 52 and 57.

(D) Method 350.1, Determination of Ammonium Nitrogen by Semi-Automated Colorimetry. Revision 2.0. Table IB, Notes 30 and 52.

(E) Method 351.2, Determination of Total Kjeldahl Nitrogen by Semi-Automated Colorimetry. Revision 2.0. Table IB, Note 52.

(F) Method 353.2, Determination of Nitrate-Nitrite Automated Colorimetry. Revision 2.0. Table IB, Note 52.

(G) Method 365.1, Determination of Phosphorus by Automated Colorimetry. Revision 2.0. Table IB, Note 52.

(H) Method 375.2, Determination of Sulfate by Automated Colorimetry. Revision 2.0. Table IB, Note 52.

(I) Method 410.4, Determination of Chemical Oxygen Demand by Semi-Automated Colorimetry. Revision 2.0. Table IB, Note 52.

(ii) Methods for the Determination of Metals in Environmental Samples, Supplement I. May 1994. EPA/600/R-94/111, Pub. No. PB 95125472. Table IB, Note 52.

(A) Method 200.7, Determination of Metals and Trace Elements in Water and Wastes by Inductively Coupled Plasma-Atomic Emission Spectrometry. Revision 4.4. Table IB, Note 52.

(B) Method 200.8, Determination of Trace Elements in Water and Wastes by Inductively Coupled Plasma Mass Spectrometry. Revision 5.3. Table IB, Note 52.

(C) Method 200.9, Determination of Trace Elements by Stabilized Temperature Graphite Furnace Atomic Absorption Spectrometry. Revision 2.2. Table IB, Note 52.

(D) Method 218.6, Determination of Dissolved Hexavalent Chromium in Drinking Water, Groundwater, and Industrial Wastewater Effluents by Ion Chromatography. Revision 3.3. Table IB, Note 52.

(E) Method 245.1, Determination of Mercury in Water by Cold Vapor Atomic Absorption Spectrometry. Revision 3.0. Table IB, Note 52.

(4) National Exposure Risk Laboratory-Cincinnati, U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm.

(i) EPA Method 200.5, Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry. Revision 4.2, October 2003. EPA/600/R-06/115. Table IB, Note 68.

(ii) EPA Method 525.2, Determination of Organic Compounds in Drinking Water by Liquid-Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry. Revision 2.0, 1995. Table ID, Note 10.

(5) Office of Research and Development, Cincinnati OH. U.S. Environmental Protection Agency, Cincinnati OH (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm or from ORD Publications, CERI, U.S. Environmental Protection Agency, Cincinnati OH 45268.

(i) Methods for Benzidine, Chlorinated Organic Compounds, Pentachlorophenol, and Pesticides in Water and Wastewater. 1978. Table IC, Note 3; Table ID, Note 3.

(ii) Methods for Chemical Analysis of Water and Wastes. March 1979. EPA-600/4-79-020. Table IB, Note 1.

(iii) Methods for Chemical Analysis of Water and Wastes. Revised March 1983. EPA-600/4-79-020. Table IB, Note 1.

(A) Method 120.1, Conductance, Specific Conductance, μmhos at 25°C. Revision 1982. Table IB, Note 1.

(B) Method 130.1, Hardness, Total (mg/L as CaCO3), Colorimetric, Automated EDTA. Issued 1971. Table IB, Note 1.

(C) Method 150.2, pH, Continuous Monitoring (Electrometric). December 1982. Table IB, Note 1.

(D) Method 160.4, Residue, Volatile, Gravimetric, Ignition at 550°C. Issued 1971. Table IB, Note 1.

(E) Method 206.5, Arsenic, Sample Digestion Prior to Total Arsenic Analysis by Silver Diethyldithiocarbamate or Hydride Procedures. Issued 1978. Table IB, Note 1.

(F) Method 231.2, Gold, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(G) Method 245.2, Mercury, Automated Cold Vapor Technique. Issued 1974. Table IB, Note 1.

(H) Method 252.2, Osmium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(I) Method 253.2, Palladium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(J) Method 255.2, Platinum, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(K) Method 265.2, Rhodium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(L) Method 279.2, Thallium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(M) Method 283.2, Titanium, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(N) Method 289.2, Zinc, Atomic Absorption, Furnace Technique. Issued 1978. Table IB, Note 1.

(O) Method 310.2, Alkalinity, Colorimetric, Automated, Methyl Orange. Revision 1974. Table IB, Note 1.

(P) Method 351.1, Nitrogen, Kjeldahl, Total, Colorimetric, Automated Phenate. Revision 1978. Table IB, Note 1.

(Q) Method 352.1, Nitrogen, Nitrate, Colorimetric, Brucine. Issued 1971. Table IB, Note 1.

(R) Method 365.3, Phosphorus, All Forms, Colorimetric, Ascorbic Acid, Two Reagent. Issued 1978. Table IB, Note 1.

(S) Method 365.4, Phosphorus, Total, Colorimetric, Automated, Block Digestor AA II. Issued 1974. Table IB, Note 1.

(T) Method 410.3, Chemical Oxygen Demand, Titrimetric, High Level for Saline Waters. Revision 1978. Table IB, Note 1.

(U) Method 420.1, Phenolics, Total Recoverable, Spectrophotometric, Manual 4-AAP With Distillation. Revision 1978. Table IB, Note 1.

(iv) Prescribed Procedures for Measurement of Radioactivity in Drinking Water. 1980. EPA-600/4-80-032. Table IE.

(A) Method 900.0, Gross Alpha and Gross Beta Radioactivity. Table IE.

(B) Method 903.0, Alpha-Emitting iRadio Isotopes. Table IE.

(C) Method 903.1, Radium-226, Radon Emanation Technique. Table IE.

(D) Appendix B, Error and Statistical Calculations. Table IE.

(6) Office of Science and Technology, U.S. Environmental Protection Agency, Washington DC (US EPA). Available athttp://water.epa.gov/scitech/methods/cwa/index.cfm.

(i) Method 1625C, Semivolatile Organic Compounds by Isotope Dilution GCMS. 1989. Table IF.

(ii) [Reserved]

(7) Office of Water, U.S. Environmental Protection Agency, Washington DC (US EPA). Available at http://water.epa.gov/scitech/methods/cwa/index.cfm or from National Technical Information Service, 5285 Port Royal Road, Springfield, Virginia 22161.

(i) Method 1631, Mercury in Water by Oxidation, Purge and Trap, and Cold Vapor Atomic Fluorescence Spectrometry. Revision E, August 2002. EPA-821-R-02-019, Pub. No. PB2002-108220. Table IB, Note 43.

(ii) Kelada-01, Kelada Automated Test Methods for Total Cyanide, Acid Dissociable Cyanide, and Thiocyanate. Revision 1.2, August 2001. EPA 821-B-01-009, Pub. No. PB 2001-108275. Table IB, Note 55.

(iii) In the compendium Analytical Methods for the Determination of Pollutants in Pharmaceutical Manufacturing Industry Wastewaters. July 1998. EPA 821-B-98-016, Pub. No. PB95201679. Table IF, Note 1.

(A) EPA Method 1666, Volatile Organic Compounds Specific to the Pharmaceutical Industry by Isotope Dilution GC/MS. Table IF, Note 1.

(B) EPA Method 1667, Formaldehyde, Isobutyraldehyde, and Furfural by Derivatization Followed by High Performance Liquid Chromatography. Table IF.

(C) Method 1671, Volatile Organic Compounds Specific to the Pharmaceutical Manufacturing Industry by GC/FID. Table IF.

(iv) Methods For The Determination of Nonconventional Pesticides In Municipal and Industrial Wastewater, Volume I. Revision I, August 1993. EPA 821-R-93-010A, Pub. No. PB 94121654. Tables ID, IG.

(A) Method 608.1, Organochlorine Pesticides. Table ID, Note 10; Table IG, Note 3.

(B) Method 608.2, Certain Organochlorine Pesticides. Table ID, Note 10; Table IG, Note 3.

(C) Method 614, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.

(D) Method 614.1, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.

(E) Method 615, Chlorinated Herbicides. Table ID, Note 10; Table IG, Note 3.

(F) Method 617, Organohalide Pesticides and PCBs. Table ID, Note 10; Table IG, Note 3.

(G) Method 619, Triazine Pesticides. Table ID, Note 10; Table IG, Note 3.

(H) Method 622, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.

(I) Method 622.1, Thiophosphate Pesticides. Table ID, Note 10; Table IG, Note 3.

(J) Method 627, Dinitroaniline Pesticides. Table ID, Note 10; Table IG, Notes 1 and 3.

(K) Method 629, Cyanazine. Table IG, Note 3.

(L) Method 630, Dithiocarbamate Pesticides. Table IG, Note 3.

(M) Method 630.1, Dithiocarbamate Pesticides. Table IG, Note 3.

(N) Method 631, Benomyl and Carbendazim. Table IG, Note 3.

(O) Method 632, Carbamate and Urea Pesticides. Table ID, Note 10; Table IG, Note 3.

(P) Method 632.1, Carbamate and Amide Pesticides. Table IG, Note 3.

(Q) Method 633, Organonitrogen Pesticides. Table IG, Note 3.

(R) Method 633.1, Neutral Nitrogen-Containing Pesticides. Table IG, Note 3.

(S) Method 637, MBTS and TCMTB. Table IG, Note 3.

(T) Method 644, Picloram. Table IG, Note 3.

(U) Method 645, Certain Amine Pesticides and Lethane. Table IG, Note 3.

(V) Method 1656, Organohalide Pesticides. Table ID, Note 10; Table IG, Notes 1 and 3.

(W) Method 1657, Organophosphorus Pesticides. Table ID, Note 10; Table IG, Note 3.

(X) Method 1658, Phenoxy-Acid Herbicides. Table IG, Note 3.

(Y) Method 1659, Dazomet. Table IG, Note 3.

(Z) Method 1660, Pyrethrins and Pyrethroids. Table IG, Note 3.

(AA) Method 1661, Bromoxynil. Table IG, Note 3.

(BB) Ind-01. Methods EV-024 and EV-025, Analytical Procedures for Determining Total Tin and Triorganotin in Wastewater. Table IG, Note 3.

(v) Methods For The Determination of Nonconventional Pesticides In Municipal and Industrial Wastewater, Volume II. August 1993. EPA 821-R-93-010B, Pub. No. PB 94166311. Table IG.

(A) Method 200.9, Determination of Trace Elements by Stabilized Temperature Graphite Furnace Atomic Absorption Spectrometry. Table IG, Note 3.

(B) Method 505, Analysis of Organohalide Pesticides and Commercial Polychlorinated Biphenyl (PCB) Products in Water by Microextraction and Gas Chromatography. Table ID, Note 10; Table IG, Note 3.

(C) Method 507, The Determination of Nitrogen- and Phosphorus-Containing Pesticides in Water by Gas Chromatography with a Nitrogen-Phosphorus Detector. Table ID, Note 10; Table IG, Note 3.

(D) Method 508, Determination of Chlorinated Pesticides in Water by Gas Chromatography with an Electron Capture Detector. Table ID, Note 10; Table IG, Note 3.

(E) Method 515.1, Determination of Chlorinated Acids in Water by Gas Chromatography with an Electron Capture Detector. Table IG, Notes 2 and 3.

(F) Method 515.2, Determination of Chlorinated Acids in Water Using Liquid-Solid Extraction and Gas Chromatography with an Electron Capture Detector. Table IG, Notes 2 and 3.

(G) Method 525.1, Determination of Organic Compounds in Drinking Water by Liquids-Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry. Table ID, Note 10; Table IG, Note 3.

(H) Method 531.1, Measurement of N-Methylcarbamoyloximes and N-Methylcarbamates in Water by Direct Aqueous Injection HPLC with Post-Column Derivatization. Table ID, Note 10; Table IG, Note 3.

(I) Method 547, Determination of Glyphosate in Drinking Water by Direct-Aqueous-Injection HPLC, Post-Column Derivatization, and Fluorescence Detection. Table IG, Note 3.

(J) Method 548, Determination of Endothall in Drinking Water by Aqueous Derivatization, Liquid-Solid Extraction, and Gas Chromatography with Electron-Capture Detector. Table IG, Note 3.

(K) Method 548.1, Determination of Endothall in Drinking Water by Ion-Exchange Extraction, Acidic Methanol Methylation and Gas Chromatography/Mass Spectrometry. Table IG, Note 3.

(L) Method 553, Determination of Benzidines and Nitrogen-Containing Pesticides in Water by Liquid-Liquid Extraction or Liquid-Solid Extraction and Reverse Phase High Performance Liquid Chromatography/Particle Beam/Mass Spectrometry Table ID, Note 10; Table IG, Note 3.

(M) Method 555, Determination of Chlorinated Acids in Water by High Performance Liquid Chromatography With a Photodiode Array Ultraviolet Detector. Table IG, Note 3.

(vi) In the compendium Methods for the Determination of Organic Compounds in Drinking Water. Revised July 1991, December 1998. EPA-600/4-88-039, Pub. No. PB92-207703. Table IF.

(A) EPA Method 502.2, Volatile Organic Compounds in Water by Purge and Trap Capillary Column Gas Chromatography with Photoionization and Electrolytic Conductivity Detectors in Series. Table IF.

(B) [Reserved]

(vii) In the compendium Methods for the Determination of Organic Compounds in Drinking Water-Supplement II. August 1992. EPA-600/R-92-129, Pub. No. PB92-207703. Table IF.

(A) EPA Method 524.2, Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry. Table IF.

(B) [Reserved]

(viii) Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, Fifth Edition. October 2002. EPA 821-R-02-012, Pub. No. PB2002-108488. Table IA, Note 26.

(ix) Short-Term Methods for Measuring the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition. October 2002. EPA 821-R-02-013, Pub. No. PB2002-108489. Table IA, Note 27.

(x) Short-Term Methods for Measuring the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, Third Edition. October 2002. EPA 821-R-02-014, Pub. No. PB2002-108490. Table IA, Note 28.

(8) Office of Water, U.S. Environmental Protection Agency (U.S. EPA), mail code 4303T, 1301 Constitution Avenue NW, Washington, DC 20460; website: www.epa.gov/cwa-methods.

(i) Method 245.7, Mercury in Water by Cold Vapor Atomic Fluorescence Spectrometry. Revision 2.0, February 2005. EPA-821-R-05-001. Table IB, Note 17.

(ii) Method 1103.2: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC), EPA-821-R-23-009. September 2023. Table IH, Note 18.

(iii) Method 1106.2: Enterococci in Water by Membrane Filtration Using membrane- Enterococcus -Esculin Iron Agar (mE-EIA), EPA-821-R-23-007. September 2023. Table IH, Note 22.

(iv) Method 1600.1: Enterococci in Water by Membrane Filtration Using membrane- Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-23-006, September 2023. Table 1A, Note 24; Table IH, Note 23.

(v) Method 1603.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-23-008, September 2023. Table IA, Note 21; Table IH, Note 19.

(vi) Method 1604: Total Coliforms and Escherichia coli ( E. coli) in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium). September 2002. EPA-821-R-02-024. Table IH, Note 21.

(vii) Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016, Table IA, Notes 25, 26, and 27.

(viii) Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. December 2005. EPA-821-R-05-002. Table IH, Note 26.

(ix) Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA 816-R-12-001. January 2012. U.S. EPA, Table IH, Notes 25 and 31.

(x) Method 1627, Kinetic Test Method for the Prediction of Mine Drainage Quality. December 2011. EPA-821-R-09-002. Table IB, Note 69.

(xi) Method 1664, n -Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n -Hexane Extractable Material (SGT-HEM; Nonpolar Material) by Extraction and Gravimetry. Revision A, February 1999. EPA-821-R-98-002. Table IB, Notes 38 and 42.

(xii) Method 1664, n -Hexane Extractable Material (HEM; Oil and Grease) and Silica Gel Treated n -Hexane Extractable Material (SGT-HEM; Nonpolar Material) by Extraction and Gravimetry, Revision B, February 2010. EPA-821-R-10-001. Table IB, Notes 38 and 42.

(xiii) Method 1669, Sampling Ambient Water for Trace Metals at EPA Water Quality Criteria Levels. July 1996. Table IB, Note 43.

(xiv) Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium. September 2014. EPA-821-R-14-009.Table IA, Note 15.

(xv) Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 Medium. July 2006. EPA 821-R-06-013. Table IA, Note 20.

(xvi) Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. September 2014. EPA 821-R-14-012. Table IA, Note 23.

(9) American National Standards Institute, 1430 Broadway, New York NY 10018.

(i) ANSI. American National Standard on Photographic Processing Effluents. April 2, 1975. Table IB, Note 9.

(ii) [Reserved]

(10) American Public Health Association, 800 I Street, NW, Washington, DC 20001; phone: (202)777-2742, website: www.standardmethods.org.

(i) Standard Methods for the Examination of Water and Wastewater. 14th Edition, 1975. Table IB, Notes 27 and 86.

(ii) Standard Methods for the Examination of Water and Wastewater. 15th Edition, 1980, Table IB, Note 30; Table ID.

(iii) Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. Table IC, Note 6; Table ID, Note 6.

(iv) Standard Methods for the Examination of Water and Wastewater. 18th Edition, 1992. Tables IA, IB, IC, ID, IE, and IH.

(v) Standard Methods for the Examination of Water and Wastewater. 19th Edition, 1995. Tables IA, IB, IC, ID, IE, and IH.

(vi) Standard Methods for the Examination of Water and Wastewater. 20th Edition, 1998. Tables IA, IB, IC, ID, IE, and IH.

(vii) Standard Methods for the Examination of Water and Wastewater. 21st Edition, 2005. Table IB, Notes 17 and 27.

(viii) 2120, Color. Revised September 4, 2021. Table IB.

(ix) 2130, Turbidity. Revised 2020. Table IB.

(x) 2310, Acidity. Revised 2020. Table IB.

(xi) 2320, Alkalinity. Revised 2021. Table IB.

(xii) 2340, Hardness. Revised 2021. Table IB.

(xiii) 2510, Conductivity. Revised 2021. Table IB.

(xiv) 2540, Solids. Revised 2020. Table IB.

(xv) 2550, Temperature. 2010. Table IB.

(xvi) 3111, Metals by Flame Atomic Absorption Spectrometry. Revised 2019. Table IB.

(xvii) 3112, Metals by Cold-Vapor Atomic Absorption Spectrometry. Revised 2020. Table IB.

(xviii) 3113, Metals by Electrothermal Atomic Absorption Spectrometry. Revised 2020. Table IB.

(xix) 3114, Arsenic and Selenium by Hydride Generation/Atomic Absorption Spectrometry. Revised 2020, Table IB.

(xx) 3120, Metals by Plasma Emission Spectroscopy. Revised 2020. Table IB.

(xxi) 3125, Metals by Inductively Coupled Plasma-Mass Spectrometry. Revised 2020. Table IB.

(xxii) 3500-Al, Aluminum. Revised 2020. Table IB.

(xxiii) 3500-As, Arsenic. Revised 2020. Table IB.

(xxiv) 3500-Ca, Calcium. Revised 2020. Table IB.

(xxv) 3500-Cr, Chromium. Revised 2020. Table IB.

(xxvi) 3500-Cu, Copper. Revised 2020. Table IB.

(xxvii) 3500-Fe, Iron. 2011. Table IB.

(xxviii) 3500-Pb, Lead. Revised 2020. Table IB.

(xxix) 3500-Mn, Manganese. Revised 2020. Table IB.

(xxx) 3500-K, Potassium. Revised 2020. Table IB.

(xxxi) 3500-Na, Sodium. Revised 2020. Table IB.

(xxxii) 3500-V, Vanadium. 2011. Table IB.

(xxxiii) 3500-Zn, Zinc. Revised 2020. Table IB.

(xxxiv) 4110, Determination of Anions by Ion Chromatography. Revised 2020. Table IB.

(xxxv) 4140, Inorganic Anions by Capillary Ion Electrophoresis. Revised 2020. Table IB.

(xxxvi) 4500-B, Boron. 2011. Table IB.

(xxxvii) 4500 Cl , Chloride. Revised 2021. Table IB.

(xxxviii) 4500-Cl, Chlorine (Residual). 2011. Table IB.

(xxxix) 4500-CN , Cyanide. Revised 2021. Table IB.

(xl) 4500-F , Fluoride. Revised 2021. Table IB.

(xli) 4500-H + , pH. 2021. Table IB.

(xlii) 4500-NH3 , Nitrogen (Ammonia). Revised 2021. Table IB.

(xliii) 4500-NO2 , Nitrogen (Nitrite). Revised 2021. Table IB.

(xliv) 4500-NO3 , Nitrogen (Nitrate). Revised 2019. Table IB.

(xlv) 4500-N(org) , Nitrogen (Organic). Revised 2021. Table IB.

(xlvi) 4500-O, Oxygen (Dissolved). Revised 2021. Table IB.

(xlvii) 4500-P, Phosphorus. Revised 2021. Table IB.

(xlviii) 4500-SiO2 , Silica. Revised 2021. Table IB.

(xlix) 4500-S2− , Sulfide. Revised 2021. Table IB.

(l) 4500-SO32− , Sulfite. Revised 2021. Table IB.

(li) 4500-SO42− , Sulfate. Revised 2021. Table IB.

(lii) 5210, Biochemical Oxygen Demand (BOD). Revised 2016. Table IB.

(liii) 5220, Chemical Oxygen Demand (COD). 2011. Table IB.

(liv) 5310, Total Organic Carbon (TOC). Revised 2014. Table IB.

(lv) 5520, Oil and Grease. Revised 2021. Table IB.

(lvi) 5530, Phenols. Revised 2021. Table IB.

(lvii) 5540, Surfactants. Revised 2021. Table IB.

(lviii) 6200, Volatile Organic Compounds. Revised 2020. Table IC.

(lix) 6410, Extractable Base/Neutrals and Acids. Revised 2020. Tables IC and ID.

(lx) 6420, Phenols. Revised 2021. Table IC.

(lxi) 6440, Polynuclear Aromatic Hydrocarbons. Revised 2021. Table IC.

(lxii) 6630, Organochlorine Pesticides. Revised 2021. Table ID.

(lxiii) 6640, Acidic Herbicide Compounds. Revised 2021. Table ID.

(lxiv) 7110, Gross Alpha and Gross Beta Radioactivity (Total, Suspended, and Dissolved). 2000. Table IE.

(lxv) 7500, Radium. 2001. Table IE.

(lxvi) 9213, Recreational Waters. 2007. Table IH.

(lxvii) 9221, Multiple-Tube Fermentation Technique for Members of the Coliform Group. Approved 2014. Table IA, Notes 12, 14; and 33; Table IH, Notes 10, 12, and 32.

(lxviii) 9222, Membrane Filter Technique for Members of the Coliform Group. 2015. Table IA, Note 31; Table IH, Note 17.

(lxix) 9223 Enzyme Substrate Coliform Test. 2016. Table IA; Table IH.

(lxx) 9230 Fecal Enterococcus/Streptococcus Groups. 2013. Table IA, Note 32; Table IH.

(11) The Analyst, The Royal Society of Chemistry, RSC Publishing, Royal Society of Chemistry, Thomas Graham House, Science Park, Milton Road, Cambridge CB4 0WF, United Kingdom. (Also available from most public libraries.)

(i) Spectrophotometric Determination of Ammonia: A Study of a Modified Berthelot Reaction Using Salicylate and Dichloroisocyanurate. Krom, M.D. 105:305-316, April 1980. Table IB, Note 60.

(ii) [Reserved]

(12) Analytical Chemistry, ACS Publications, 1155 Sixteenth St. NW., Washington DC 20036. (Also available from most public libraries.)

(i) Spectrophotometric and Kinetics Investigation of the Berthelot Reaction for the Determination of Ammonia. Patton, C.J. and S.R. Crouch. 49(3):464-469, March 1977. Table IB, Note 60.

(ii) [Reserved]

(13) AOAC International, 481 North Frederick Avenue, Suite 500, Gaithersburg, MD 20877-2417.

(i) Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, 1998.

(A) 920.203, Manganese in Water, Persulfate Method. Table IB, Note 3.

(B) 925.54, Sulfate in Water, Gravimetric Method. Table IB, Note 3.

(C) 973.40, Specific Conductance of Water. Table IB, Note 3.

(D) 973.41, pH of Water. Table IB, Note 3.

(E) 973.43, Alkalinity of Water, Titrimetric Method. Table IB, Note 3.

(F) 973.44, Biochemical Oxygen Demand (BOD) of Water, Incubation Method. Table IB, Note 3.

(G) 973.45, Oxygen (Dissolved) in Water, Titrimetric Methods. Table IB, Note 3.

(H) 973.46, Chemical Oxygen Demand (COD) of Water, Titrimetric Methods. Table IB, Note 3.

(I) 973.47, Organic Carbon in Water, Infrared Analyzer Method. Table IB, Note 3.

(J) 973.48, Nitrogen (Total) in Water, Kjeldahl Method. Table IB, Note 3.

(K) 973.49, Nitrogen (Ammonia) in Water, Colorimetric Method. Table IB, Note 3.

(L) 973.50, Nitrogen (Nitrate) in Water, Brucine Colorimetric Method. Table IB, Note 3.

(M) 973.51, Chloride in Water, Mercuric Nitrate Method. Table IB, Note 3.

(N) 973.52, Hardness of Water. Table IB, Note 3.

(O) 973.53, Potassium in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.

(P) 973.54, Sodium in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.

(Q) 973.55, Phosphorus in Water, Photometric Method. Table IB, Note 3.

(R) 973.56, Phosphorus in Water, Automated Method. Table IB, Note 3.

(S) 974.27, Cadmium, Chromium, Copper, Iron, Lead, Magnesium, Manganese, Silver, Zinc in Water, Atomic Absorption Spectrophotometric Method. Table IB, Note 3.

(T) 977.22, Mercury in Water, Flameless Atomic Absorption Spectrophotometric Method. Table IB, Note 3.

(U) 991.15. Total Coliforms and Escherichia coli in Water Defined Substrate Technology (Colilert) Method. Table IA, Note 10; Table IH, Note 10.

(V) 993.14, Trace Elements in Waters and Wastewaters, Inductively Coupled Plasma-Mass Spectrometric Method. Table IB, Note 3.

(W) 993.23, Dissolved Hexavalent Chromium in Drinking Water, Ground Water, and Industrial Wastewater Effluents, Ion Chromatographic Method. Table IB, Note 3.

(X) 993.30, Inorganic Anions in Water, Ion Chromatographic Method. Table IB, Note 3.

(ii) [Reserved]

(14) Applied and Environmental Microbiology, American Society for Microbiology, 1752 N Street NW., Washington DC 20036. (Also available from most public libraries.)

(i) New Medium for the Simultaneous Detection of Total Coliforms and Escherichia coli in Water. Brenner, K.P., C.C. Rankin, Y.R. Roybal, G.N. Stelma, Jr., P.V. Scarpino, and A.P. Dufour. 59:3534-3544, November 1993. Table IH, Note 21.

(ii) [Reserved]

(15) ASTM International, 100 Barr Harbor Drive, P.O. Box C700, West Conshohocken, PA 19428-2959; phone: (877)909-2786; website: www.astm.org.

(i) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1994. Tables IA, IB, IC, ID, IE, and IH.

(ii) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1996. Tables IA, IB, IC, ID, IE, and IH.

(iii) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 1999. Tables IA, IB, IC, ID, IE, and IH.

(iv) Annual Book of ASTM Standards, Water, and Environmental Technology, Section 11, Volumes 11.01 and 11.02. 2000. Tables IA, IB, IC, ID, IE, and IH.

(v) ASTM D511-14, Standard Test Methods for Calcium and Magnesium in Water. Approved October 1, 2014. Table IB.

(vi) ASTM D512-12, Standard Test Methods for Chloride Ion in Water. Approved June 15, 2012. Table IB.

(vii) ASTM D515-88, Test Methods for Phosphorus in Water, March 1989. Table IB.

(viii) ASTM D516-16, Standard Test Method for Sulfate Ion in Water. Approved June 1, 2016. Table IB.

(ix) ASTM D858-17, Standard Test Methods for Manganese in Water. Approved June 1, 2017. Table IB.

(x) ASTM D859-16, Standard Test Method for Silica in Water. Approved June 15, 2016. Table IB.

(xi) ASTM D888-18, Standard Test Methods for Dissolved Oxygen in Water. Approved May 1, 2018. Table IB.

(xii) ASTM D1067-16, Standard Test Methods for Acidity or Alkalinity of Water. Approved June 15, 2016. Table IB.

(xiii) ASTM D1068-15, Standard Test Methods for Iron in Water. Approved October 1, 2015. Table IB.

(xiv) ASTM D1125-95 (Reapproved 1999), Standard Test Methods for Electrical Conductivity and Resistivity of Water. December 1995. Table IB.

(xv) ASTM D1126-17, Standard Test Method for Hardness in Water. Approved December 1, 2017. Table IB.

(xvi) ASTM D1179-16, Standard Test Methods for Fluoride Ion in Water. Approved June 15, 2016. Table IB.

(xvii) ASTM D1246-16, Standard Test Method for Bromide Ion in Water. June 15, 2016. Table IB.

(xviii) ASTM D1252-06 (Reapproved 2012), Standard Test Methods for Chemical Oxygen Demand (Dichromate Oxygen Demand) of Water. Approved June 15, 2012. Table IB.

(xix) ASTM D1253-14, Standard Test Method for Residual Chlorine in Water. Approved January 15, 2014. Table IB.

(xx) ASTM D1293-18, Standard Test Methods for pH of Water. Approved January 15, 2018. Table IB.

(xxi) ASTM D1426-15, Standard Test Methods for Ammonia Nitrogen in Water. Approved March 15, 2015. Table IB.

(xxii) ASTM D1687-17, Standard Test Methods for Chromium in Water. Approved June 1, 2017. Table IB.

(xxiii) ASTM D1688-17, Standard Test Methods for Copper in Water. Approved June 1, 2017. Table IB.

(xxiv) ASTM D1691-17, Standard Test Methods for Zinc in Water. Approved June 1, 2017. Table IB.

(xxv) ASTM D1783-01 (Reapproved 2012), Standard Test Methods for Phenolic Compounds in Water. Approved June 15, 2012. Table IB.

(xxvi) ASTM D1886-14, Standard Test Methods for Nickel in Water. Approved October 1, 2014. Table IB.

(xxvii) ASTM D1889-00, Standard Test Method for Turbidity of Water. October 2000. Table IB.

(xxviii) ASTM D1890-96, Standard Test Method for Beta Particle Radioactivity of Water. April 1996. Table IE.

(xxix) ASTM D1943-96, Standard Test Method for Alpha Particle Radioactivity of Water. April 1996. Table IE.

(xxx) ASTM D1976-20, Standard Test Method for Elements in Water by Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy. Approved May 1, 2020. Table IB.

(xxxi) ASTM D2036-09 (Reapproved 2015), Standard Test Methods for Cyanides in Water. Approved July 15, 2015. Table IB.

(xxxii) ASTM D2330-20, Standard Test Method for Methylene Blue Active Substances. Approved January 1, 2020. Table 1B.

(xxxiii) ASTM D2460-97, Standard Test Method for Alpha-Particle-Emitting Isotopes of Radium in Water. October 1997. Table IE.

(xxxiv) ASTM D2972-15, Standard Tests Method for Arsenic in Water. Approved February 1, 2015. Table IB.

(xxxv) ASTM D3223-17, Standard Test Method for Total Mercury in Water. Approved June 1, 2017. Table IB.

(xxxvi) ASTM D3371-95, Standard Test Method for Nitriles in Aqueous Solution by Gas-Liquid Chromatography, February 1996. Table IF.

(xxxvii) ASTM D3373-17, Standard Test Method for Vanadium in Water. Approved June 1, 2017. Table IB.

(xxxviii) ASTM D3454-97, Standard Test Method for Radium-226 in Water. February 1998. Table IE.

(xxxix) ASTM D3557-17, Standard Test Method for Cadmium in Water. Approved June 1, 2017. Table IB.

(xl) ASTM D3558-15, Standard Test Method for Cobalt in Water. Approved February 1, 2015. Table IB.

(xli) ASTM D3559-15, Standard Test Methods for Lead in Water. Approved June 1, 2015. Table IB.

(xlii) ASTM D3590-17, Standard Test Methods for Total Kjeldahl Nitrogen in Water. Approved June 1, 2017. Table IB.

(xliii) ASTM D3645-15, Standard Test Methods for Beryllium in Water. Approved February 1, 2015. Table IB.

(xliv) ASTM D3695-95, Standard Test Method for Volatile Alcohols in Water by Direct Aqueous-Injection Gas Chromatography. April 1995. Table IF.

(xlv) ASTM D3859-15, Standard Test Methods for Selenium in Water. Approved March 15, 2015. Table IB.

(xlvi) ASTM D3867-16, Standard Test Method for Nitrite-Nitrate in Water. Approved June 1, 2016. Table IB.

(xlvii) ASTM D4190-15, Standard Test Method for Elements in Water by Direct- Current Plasma Atomic Emission Spectroscopy. Approved February 1, 2015. Table IB.

(xlviii) ASTM D4282-15, Standard Test Method for Determination of Free Cyanide in Water and Wastewater by Microdiffusion. Approved July 15, 2015. Table IB.

(xlix) ASTM D4327-17, Standard Test Method for Anions in Water by Suppressed Ion Chromatography. Approved December 1, 2017. Table IB.

(l) ASTM D4382-18, Standard Test Method for Barium in Water, Atomic Absorption Spectrophotometry, Graphite Furnace. Approved February 1, 2018. Table IB.

(li) ASTM D4657-92 (Reapproved 1998), Standard Test Method for Polynuclear Aromatic Hydrocarbons in Water. January 1993. Table IC.

(lii) ASTM D4658-15, Standard Test Method for Sulfide Ion in Water. Approved March 15, 2015. Table IB.

(liii) ASTM D4763-88 (Reapproved 2001), Standard Practice for Identification of Chemicals in Water by Fluorescence Spectroscopy. September 1988. Table IF.

(liv) ASTM D4839-03 (Reapproved 2017), Standard Test Method for Total Carbon and Organic Carbon in Water by Ultraviolet, or Persulfate Oxidation, or Both, and Infrared Detection. Approved December 15, 2017. Table IB.

(lv) ASTM D5257-17, Standard Test Method for Dissolved Hexavalent Chromium in Water by Ion Chromatography. Approved December 1, 2017. Table IB.

(lvi) ASTM D5259-92, Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure. October 1992. Table IH, Note 9.

(lvii) ASTM D5392-93, Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure. September 1993. Table IH, Note 9.

(lviii) ASTM D5673-16, Standard Test Method for Elements in Water by Inductively Coupled Plasma—Mass Spectrometry. Approved February 1, 2016. Table IB.

(lix) ASTM D5907-18, Standard Test Methods for Filterable Matter (Total Dissolved Solids) and Nonfilterable Matter (Total Suspended Solids) in Water. Approved May 1, 2018. Table IB.

(lx) ASTM D6503-99, Standard Test Method for Enterococci in Water Using Enterolert. April 2000. Table IA Note 9, Table IH, Note 9.

(lxi) ASTM. D6508-15, Standard Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. Approved October 1, 2015. Table IB, Note 54.

(lxii) ASTM. D6888-16, Standard Test Method for Available Cyanides with Ligand Displacement and Flow Injection Analysis (FIA) Utilizing Gas Diffusion Separation and Amperometric Detection. Approved February 1, 2016. Table IB, Note 59.

(lxiii) ASTM. D6919-17, Standard Test Method for Determination of Dissolved Alkali and Alkaline Earth Cations and Ammonium in Water and Wastewater by Ion Chromatography. Approved June 1, 2017. Table IB.

(lxiv) ASTM. D7065-17, Standard Test Method for Determination of Nonylphenol, Bisphenol A, p-tert -Octylphenol, Nonylphenol Monoethoxylate and Nonylphenol Diethoxylate in Environmental Waters by Gas Chromatography Mass Spectrometry. Approved December 15, 2017. Table IC.

(lxv) ASTM D7237-18, Standard Test Method for Free Cyanide with Flow Injection Analysis (FIA) Utilizing Gas Diffusion Separation and Amperometric Detection. Approved December 1, 2018. Table IB.

(lxvi) ASTM D7284-20, Standard Test Method for Total Cyanide in Water by Micro Distillation followed by Flow Injection Analysis with Gas Diffusion Separation and Amperometric Detection. Approved August 1, 2020. Table IB.

(lxvii) ASTM D7365-09a (Reapproved 2015), Standard Practice for Sampling, Preservation and Mitigating Interferences in Water Samples for Analysis of Cyanide. Approved July 15, 2015. Table II, Notes 5 and 6.

(lxviii) ASTM. D7511-12 (Reapproved 2017) e1 , Standard Test Method for Total Cyanide by Segmented Flow Injection Analysis, In-Line Ultraviolet Digestion and Amperometric Detection. Approved July 1, 2017. Table IB.

(lxix) ASTM D7573-18a e1 , Standard Test Method for Total Carbon and Organic Carbon in Water by High Temperature Catalytic Combustion and Infrared Detection. Approved December 15, 2018. Table IB.

(lxx) ASTM D7781-14, Standard Test Method for Nitrite-Nitrate in Water by Nitrate Reductase, Approved April 1, 2014. Table IB.

(16) Bran & Luebbe Analyzing Technologies, Inc., Elmsford NY 10523.

(i) Industrial Method Number 378-75WA, Hydrogen Ion (pH) Automated Electrode Method, Bran & Luebbe (Technicon) Auto Analyzer II. October 1976. Table IB, Note 21.

(ii) [Reserved]

(17) CEM Corporation, P.O. Box 200, Matthews NC 28106-0200.

(i) Closed Vessel Microwave Digestion of Wastewater Samples for Determination of Metals. April 16, 1992. Table IB, Note 36.

(ii) [Reserved]

(18) Craig R. Chinchilla, 900 Jorie Blvd., Suite 35, Oak Brook IL 60523. Telephone: 630-645-0600.

(i) Nitrate by Discrete Analysis Easy (1-Reagent) Nitrate Method, (Colorimetric, Automated, 1 Reagent). Revision 1, November 12, 2011. Table IB, Note 62.

(ii) [Reserved]

(19) FIAlab Instruments, Inc., 334 2151 N. Northlake Way, Seattle, WA 98103; phone: (425)376-0450; website: www.flowinjection.com/app-notes/epafialab100.

(i) FIAlab 100, Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Fluorescence Detector Analysis, April 4, 2018. Table IB, Note 82.

(ii) [Reserved]

(20) Hach Company, P.O. Box 389, Loveland CO 80537.

(i) Method 8000, Chemical Oxygen Demand. Hach Handbook of Water Analysis. 1979. Table IB, Note 14.

(ii) Method 8008, 1,10-Phenanthroline Method using FerroVer Iron Reagent for Water. 1980. Table IB, Note 22.

(iii) Method 8009, Zincon Method for Zinc. Hach Handbook for Water Analysis. 1979. Table IB, Note 33.

(iv) Method 8034, Periodate Oxidation Method for Manganese. Hach Handbook for Water Analysis. 1979. Table IB, Note 23.

(v) Method 8506, Bicinchoninate Method for Copper. Hach Handbook of Water Analysis. 1979. Table IB, Note 19.

(vi) Method 8507, Nitrogen, Nitrite-Low Range, Diazotization Method for Water and Wastewater. 1979. Table IB, Note 25.

(vii) Method 10206, Hach Company TNTplus 835/836 Nitrate Method 10206, Spectrophotometric Measurement of Nitrate in Water and Wastewater. Revision 2.1, January 10, 2013. Table IB, Note 75.

(viii) Method 10242, Hach Company TNTplus 880 Total Kjeldahl Nitrogen Method 10242, Simplified Spectrophotometric Measurement of Total Kjeldahl Nitrogen in Water and Wastewater. Revision 1.1, January 10, 2013. Table IB, Note 76.

(ix) Hach Method 10360, Luminescence Measurement of Dissolved Oxygen in Water and Wastewater and for Use in the Determination of BOD5and cBOD5. Revision 1.2, October 2011. Table IB, Note 63.

(x) m-ColiBlue24® Method, for total Coliforms and E. coli. Revision 2, 1999. Table IA, Note 18; Table IH, Note 17.

(21) IDEXX Laboratories Inc., One Idexx Drive, Westbrook ME 04092.

(i) Colilert. 2013. Table IA, Notes 17 and 18; Table IH, Notes 14, 15 and 16.

(ii) Colilert-18. 2013. Table IA, Notes 17 and 18; Table IH, Notes 14, 15 and 16.

(iii) Enterolert. 2013. Table IA, Note 24; Table IH, Note 12.

(iv) Quanti-Tray Insert and Most Probable Number (MPN) Table. 2013. Table IA, Note 18; Table IH, Notes 14 and 16.

(22) In-Situ Incorporated, 221 E. Lincoln Ave., Ft. Collins CO 80524. Telephone: 970-498-1500.

(i) In-Situ Inc. Method 1002-8-2009, Dissolved Oxygen Measurement by Optical Probe. 2009. Table IB, Note 64.

(ii) In-Situ Inc. Method 1003-8-2009, Biochemical Oxygen Demand (BOD) Measurement by Optical Probe. 2009. Table IB, Note 10.

(iii) In-Situ Inc. Method 1004-8-2009, Carbonaceous Biochemical Oxygen Demand (CBOD) Measurement by Optical Probe. 2009. Table IB, Note 35.

(23) Journal of Chromatography, Elsevier/North-Holland, Inc., Journal Information Centre, 52 Vanderbilt Avenue, New York NY 10164. (Also available from most public libraries.

(i) Direct Determination of Elemental Phosphorus by Gas-Liquid Chromatography. Addison, R.F. and R.G. Ackman. 47(3): 421-426, 1970. Table IB, Note 28.

(ii) [Reserved]

(24) Lachat Instruments, 6645 W. Mill Road, Milwaukee WI 53218, Telephone: 414-358-4200.

(i) QuikChem Method 10-204-00-1-X, Digestion and Distillation of Total Cyanide in Drinking and Wastewaters using MICRO DIST and Determination of Cyanide by Flow Injection Analysis. Revision 2.2, March 2005. Table IB, Note 56.

(ii) [Reserved]

(25) Leck Mitchell, Ph.D., P.E., 656 Independence Valley Dr., Grand Junction CO 81507. Telephone: 970-244-8661.

(i) Mitchell Method M5271, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Table IB, Note 66.

(ii) Mitchell Method M5331, Determination of Turbidity by Nephelometry. Revision 1.0, July 31, 2008. Table IB, Note 65.

(26) MACHEREY-NAGEL GmbH and Co., 2850 Emrick Blvd., Bethlehem, PA 18020; Phone: (888)321-6224.

(i) Method 036/038 NANOCOLOR® COD LR/HR, Spectrophotometric Measurement of Chemical Oxygen Demand in Water and Wastewater, Revision 1.5, May 2018. Table IB, Note 83.

(ii) [Reserved]

(27) Micrology Laboratories, LLC (now known as Roth Bioscience, LLC), 1303 Eisenhower Drive, Goshen, IN 46526; phone: (574)533-3351.

(i) KwikCountTM EC Medium E. coli enzyme substrate test, Rapid Detection of E. coli in Beach Water By KwikCount TM EC Membrane Filtration. 2014. Table IH, Notes 28 and 29.

(ii) [Reserved]

(28) National Council of the Paper Industry for Air and Stream Improvements, Inc. (NCASI), 260 Madison Avenue, New York NY 10016.

(i) NCASI Method TNTP-W10900, Total Nitrogen and Total Phophorus in Pulp and Paper Biologically Treated Effluent by Alkaline Persulfate Digestion. June 2011. Table IB, Note 77.

(ii) NCASI Technical Bulletin No. 253, An Investigation of Improved Procedures for Measurement of Mill Effluent and Receiving Water Color. December 1971. Table IB, Note 18.

(iii) NCASI Technical Bulletin No. 803, An Update of Procedures for the Measurement of Color in Pulp Mill Wastewaters. May 2000. Table IB, Note 18.

(29) The Nitrate Elimination Co., Inc. (NECi), 334 Hecla St., Lake Linden NI 49945.

(i) NECi Method N07-0003, Method for Nitrate Reductase Nitrate-Nitrogen Analysis. Revision 9.0. March 2014. Table IB, Note 73.

(ii) [Reserved]

(30) Oceanography International Corporation, 512 West Loop, P.O. Box 2980, College Station TX 77840.

(i) OIC Chemical Oxygen Demand Method. 1978. Table IB, Note 13.

(ii) [Reserved]

(31) OI Analytical, Box 9010, College Station TX 77820-9010.

(i) Method OIA-1677-09, Available Cyanide by Ligand Exchange and Flow Injection Analysis (FIA). Copyright 2010. Table IB, Note 59.

(ii) Method PAI-DK01, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Titrimetric Detection. Revised December 22, 1994. Table IB, Note 39.

(iii) Method PAI-DK02, Nitrogen, Total Kjeldahl, Block Digestion, Steam Distillation, Colorimetric Detection. Revised December 22, 1994. Table IB, Note 40.

(iv) Method PAI-DK03, Nitrogen, Total Kjeldahl, Block Digestion, Automated FIA Gas Diffusion. Revised December 22, 1994. Table IB, Note 41.

(32) ORION Research Corporation, 840 Memorial Drive, Cambridge, Massachusetts 02138.

(i) ORION Research Instruction Manual, Residual Chlorine Electrode Model 97-70. 1977. Table IB, Note 16.

(ii) [Reserved]

(33) Pace Analytical Services, LLC, 1800 Elm Street, SE, Minneapolis, MN 55414; phone: (612)656-2240.

(i) PAM-16130-SSI, Determination of 2,3,7,8-Substituted Tetra- through Octa-Chlorinated Dibenzo- p -Dioxins and Dibenzofurans (CDDs/CDFs) Using Shimadzu Gas Chromatography Mass Spectrometry (GC-MS/MS), Revision 1.1, May 20, 2022. Table IC, Note 17.

(ii) [Reserved]

(34) SGS AXYS Analytical Services, Ltd., 2045 Mills Road, Sidney, British Columbia, Canada, V8L 5X2; phone: (888)373-0881.

(i) SGS AXYS Method 16130, Determination of 2,3,7,8-Substituted Tetra- through Octa-Chlorinated Dibenzo- p -Dioxins and Dibenzofurans (CDDs/CDFs) Using Waters and Agilent Gas Chromatography-Mass Spectrometry (GC/MS/MS)., Revision 1.0, revised August 2020. Table IC, Note 16.

(ii) [Reserved]

(35) Technicon Industrial Systems, Tarrytown NY 10591.

(i) Industrial Method Number 379-75WE Ammonia, Automated Electrode Method, Technicon Auto Analyzer II. February 19, 1976. Table IB, Note 7.

(ii) [Reserved]

(36) Thermo Jarrell Ash Corporation, 27 Forge Parkway, Franklin MA 02038.

(i) Method AES0029. Direct Current Plasma (DCP) Optical Emission Spectrometric Method for Trace Elemental Analysis of Water and Wastes. 1986, Revised 1991. Table IB, Note 34.

(ii) [Reserved]

(37) Thermo Scientific, 166 Cummings Center, Beverly MA 01915. Telephone: 1-800-225-1480. www.thermoscientific.com.

(i) Thermo Scientific Orion Method AQ4500, Determination of Turbidity by Nephelometry. Revision 5, March 12, 2009. Table IB, Note 67.

(ii) [Reserved]

(38) 3M Corporation, 3M Center Building 220-9E-10, St. Paul MN 55144-1000.

(i) Organochlorine Pesticides and PCBs in Wastewater Using EmporeTMDisk” Test Method 3M 0222. Revised October 28, 1994. Table IC, Note 8; Table ID, Note 8.

(ii) [Reserved]

(39) Timberline Instruments, LLC, 1880 South Flatiron Ct., Unit I, Boulder CO 80301.

(i) Timberline Amonia-001, Determination of Inorganic Ammonia by Continuous Flow Gas Diffusion and Conductivity Cell Analysis. June 24, 2011. Table IB, Note 74.

(ii) [Reserved]

(40) U.S. Geological Survey (USGS), U.S. Department of the Interior, Reston, Virginia. Available from USGS Books and Open-File Reports (OFR) Section, Federal Center, Box 25425, Denver, CO 80225; phone: (703)648-5953; website: ww.usgs.gov.

(i) Colorimetric determination of nitrate plus nitrite in water by enzymatic reduction, automated discrete analyzer methods. U.S. Geological Survey Techniques and Methods, Book 5—Laboratory Analysis, Section B—Methods of the National Water Quality Laboratory, Chapter 8. 2011. Table IB, Note 72.

(ii) Techniques and Methods—Book 5, Laboratory Analysis—Section B, Methods of the National Water Quality Laboratory—Chapter 12, Determination of Heat Purgeable and Ambient Purgeable Volatile Organic Compounds in Water by Gas Chromatography/Mass Spectrometry 2016.

(iii) Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, editors, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1979. Table IB, Note 8.

(iv) Methods for Determination of Inorganic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A1. 1989. Table IB, Notes 2 and 79.

(v) Methods for the Determination of Organic Substances in Water and Fluvial Sediments. Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A3. 1987. Table IB, Note 24; Table ID, Note 4.

(vi) OFR 76-177, Selected Methods of the U.S. Geological Survey of Analysis of Wastewaters. 1976. Table IE, Note 2.

(vii) OFR 91-519, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Organonitrogen Herbicides in Water by Solid-Phase Extraction and Capillary-Column Gas Chromatography/Mass Spectrometry With Selected-Ion Monitoring. 1992. Table ID, Note 14.

(viii) OFR 92-146, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Total Phosphorus by a Kjeldahl Digestion Method and an Automated Colorimetric Finish That Includes Dialysis. 1992. Table IB, Note 48.

(ix) OFR 93-125, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. Table IB, Notes 51 and 80; Table IC, Note 9.

(x) OFR 93-449, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Chromium in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1993. Table IB, Note 46.

(xi) OFR 94-37, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Triazine and Other Nitrogen-containing Compounds by Gas Chromatography with Nitrogen Phosphorus Detectors. 1994. Table ID, Note 9.

(xii) OFR 95-181, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by C-18 Solid-Phase Extraction and Capillary-Column Gas Chromatography/Mass Spectrometry With Selected-Ion Monitoring. 1995. Table ID, Note 11.

(xiii) OFR 97-198, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Molybdenum in Water by Graphite Furnace Atomic Absorption Spectrophotometry. 1997. Table IB, Note 47.

(xiv) OFR 97-829, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of 86 Volatile Organic Compounds in Water by Gas Chromatography/Mass Spectrometry, Including Detections Less Than Reporting Limits. 1998. Table IC, Note 13.

(xv) OFR 98-165, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Elements in Whole-Water Digests Using Inductively Coupled Plasma-Optical Emission Spectrometry and Inductively Coupled Plasma-Mass Spectrometry. 1998. Table IB, Notes 50 and 81.

(xvi) OFR 98-639, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Arsenic and Selenium in Water and Sediment by Graphite Furnace—Atomic Absorption Spectrometry. 1999. Table IB, Note 49.

(xvii) OFR 00-170, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Ammonium Plus Organic Nitrogen by a Kjeldahl Digestion Method and an Automated Photometric Finish that Includes Digest Cleanup by Gas Diffusion. 2000. Table IB, Note 45.

(xviii) Techniques and Methods Book 5-B1, Determination of Elements in Natural-Water, Biota, Sediment and Soil Samples Using Collision/Reaction Cell Inductively Coupled Plasma-Mass Spectrometry. Chapter 1, Section B, Methods of the National Water Quality Laboratory, Book 5, Laboratory Analysis. 2006. Table IB, Note 70.

(xix) U.S. Geological Survey Techniques of Water-Resources Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. Table IA, Note 4; Table IH, Note 4.

(xx) Water-Resources Investigation Report 01-4098, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Moderate-Use Pesticides and Selected Degradates in Water by C-18 Solid-Phase Extraction and Gas Chromatography/Mass Spectrometry. 2001. Table ID, Note 13.

(xxi) Water-Resources Investigations Report 01-4132, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Organic Plus Inorganic Mercury in Filtered and Unfiltered Natural Water With Cold Vapor-Atomic Fluorescence Spectrometry. 2001. Table IB, Note 71.

(xxii) Water-Resources Investigation Report 01-4134, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by Graphitized Carbon-Based Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass Spectrometry. 2001. Table ID, Note 12.

(xxiii) Water Temperature—Influential Factors, Field Measurement and Data Presentation, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 1, Chapter D1. 1975. Table IB, Note 32.

(41) Waters Corporation, 34 Maple Street, Milford MA 01757, Telephone: 508-482-2131, Fax: 508-482-3625. (i) Method D6508, Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. Revision 2, December 2000. Table IB, Note 54. (ii) [Reserved]

(i) Method D6508, Test Method for Determination of Dissolved Inorganic Anions in Aqueous Matrices Using Capillary Ion Electrophoresis and Chromate Electrolyte. Revision 2, December 2000. Table IB, Note 54.

(ii) [Reserved]

* * * *

(e)

* * * *

Table II—Required Containers, Preservation Techniques, and Holding Times

* * * *

5 ASTM D7365-09a (15) specifies treatment options for samples containing oxidants (e.g., chlorine) for cyanide analyses. Also, Section 9060A of Standard Methods for the Examination of Water and Wastewater (23rd edition) addresses dechlorination procedures for microbiological analyses.

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Most Recent Highlights In Environmental

Protecting yourself all season — All masks are not created equal!
2024-05-16T05:00:00Z

Protecting yourself all season — All masks are not created equal!

As the common cold, flu, and even lingering cases of COVID rear their heads throughout the year, many may reach for a mask as a precaution. But are your masks going to offer the protection you need? Employers must understand the differences between the various masks workers depend on for their safety.

Although most mask mandates have been lifted, the Centers for Disease Control (CDC) still recommends wearing quality masks over the nose and mouth in high traffic or congested areas, such as airplanes, at concerts, or in large groups. With many mask options floating around, it’s important to understand the differences between N95, KN95, cloth, and surgical masks.

Which mask is best?

According to the National Institute of Health (NIH), masks are intended to block respiratory droplets and aerosols that may contain viral particles. Overall, masks can be effective and are recommended during flu and cold season, but which mask is best is going to depend upon the circumstances.

Masks differ in the maximum internal leak proportion limit (the highest level of leak protection offered), so here’s the low-down:

  • N95— deemed more effective than surgical masks, N95 masks are the most common type of particulate filtering facepiece respirator. The “95” represents the 95% of airborne particles that are filtered out, which means that don’t entirely block the transmission of some viruses. These masks require fit testing to ensure proper protection.
  • KN95 — “KN” is a designation given by China as an indicator of effectiveness; though the designation is not as stringent as with N95 masks and therefore don’t qualify as N95s. These can be quickly identified by the ear loops versus the dual straps required on N95s.
  • Surgical — meant to protect from droplets and sprays, surgical masks filter large particles from the air when the wearer inhales. However, these disposable masks tend to be loose-fitting which could reduce their efficiency to lower than that of N95 masks.
  • Cloth— usually homemade, these masks offer limited protection from infection, especially persistent ones like COVID-19. Cloth masks are machine-washable and reusable, but they do fit more loosely especially near the nose, cheeks, and jawline, which reduces their effectiveness. Homemade masks create inconsistent and unknown protection levels, so are not recommended for sufficient defense against viruses or other infectious particles.
  • Other protections — scarfs, gators, and other protections have been used as an attempt to protect from contagious infections. Intended to trap respiratory droplets, these “masks” must be used in layers to have the same effectiveness as a mask, so are not the best option.

Overcoming the negatives of wearing a mask

The theory is that wearing some mask is better than wearing no mask at all. However, the use of some masks, such as N-95s can result in some not-so-pleasant physical responses such as anxiety, headaches, nausea, and vomiting. Additionally, wearing the wrong mask or incorrectly wearing a mask can create a false sense of security, placing the wearer at even greater risk. So, how can workers overcome the negatives of wearing a mask?

When wearing masks, workers can ease the “burden” by:

  • Selecting the most protective, yet comfortable mask for the expected exposure type.
  • Ensuring the mask is fit properly (around the face with no gaps around the edges) to help with air flow.
  • Using masks with bendable nose strips for efficacy and comfort.
  • Securing both straps around the ears to ensure optimal protection and prevent constant slippage.
  • Acclimating to mask use by wearing one for short periods initially and building up to longer durations.
  • Remaining aware of temperature extremes and humidity when required to wear masks.
  • Keeping your respiratory system fit and healthy so masks don’t seem like a barrier to normal breathing.

Keys to Remember: While surgical and cloth masks offer some protection from viruses, N95s and KN95s masks offer better protection in most cases. Employers must ensure workers are choosing masks that fit and filter properly to ensure safe use and appropriate level of protection.

2021-12-27T06:00:00Z

86 FR 73131 Revisions to the Unregulated Contaminant Monitoring Rule (UCMR 5) for Public Water Systems and Announcement of Public Meetings

ENVIRONMENTAL PROTECTION AGENCY

40 CFR Part 141

[EPA-HQ-OW-2020-0530; FRL-6791-03-OW]

RIN 2040-AF89

Revisions to the Unregulated Contaminant Monitoring Rule (UCMR 5) for Public Water Systems and Announcement of Public Meetings

AGENCY: Environmental Protection Agency (EPA).

ACTION: Final rule and notice of public meetings.

SUMMARY: The U.S. Environmental Protection Agency (EPA) is finalizing a Safe Drinking Water Act (SDWA) rule that requires certain public water systems (PWSs) to collect national occurrence data for 29 per- and polyfluoroalkyl substances (PFAS) and lithium. Subject to the availability of appropriations, EPA will include all systems serving 3,300 or more people and a representative sample of 800 systems serving 25 to 3,299 people. If EPA does not receive the appropriations needed for monitoring all of these systems in a given year, EPA will reduce the number of systems serving 25 to 10,000 people that will be asked to perform monitoring. This final rule is a key action to ensure science-based decision-making and prioritize protection of disadvantaged communities in accordance with EPA's PFAS Strategic Roadmap. EPA is also announcing plans for public webinars to discuss implementation of the fifth Unregulated Contaminant Monitoring Rule (UCMR 5).

DATES: This final rule is effective on January 26, 2022. The incorporation by reference of certain publications listed in this final rule is approved by the Director of the Federal Register as of January 26, 2022.

ADDRESSES: EPA has established a docket for this action under Docket ID No. EPA-HQ-OW-2020-0530. All documents in the docket are listed on the https://www.regulations.gov website. Although listed in the index, some information is not publicly available, e.g., CBI or other information whose disclosure is restricted by statute. Certain other material, such as copyrighted material, is not placed on the internet and will be publicly available only in hard copy form. Publicly available docket materials are available electronically through https://www.regulations.gov.

FOR FURTHER INFORMATION CONTACT: Brenda D. Bowden, Standards and Risk Management Division (SRMD), Office of Ground Water and Drinking Water (OGWDW) (MS 140), Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, Ohio 45268; telephone number: (513) 569-7961; email address: bowden.brenda@epa.gov; or Melissa Simic, SRMD, OGWDW (MS 140), Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, Ohio 45268; telephone number: (513) 569-7864; email address: simic.melissa@epa.gov. For general information, visit the Ground Water and Drinking Water web page at: https://www.epa.gov/ground-water-and-drinking-water.

SUPPLEMENTARY INFORMATION:

Table of Contents

I. Summary Information

A. Purpose of the Regulatory Action

1. What action is EPA taking?

2. Does this action apply to me?

3. What is EPA's authority for taking this action?

4. What is the applicability date?

B. Summary of the Regulatory Action

C. Economic Analysis

1. What is the estimated cost of this action?

2. What are the benefits of this action?

II. Public Participation

A. What meetings have been held in preparation for UCMR 5?

B. How do I participate in the upcoming meetings?

1. Meeting Participation

2. Meeting Materials

III. General Information

A. How are CCL, UCMR, Regulatory Determination process, and NCOD interrelated?

B. What are the Consumer Confidence Reporting and Public Notice Reporting requirements for public water systems that are subject to UCMR?

C. What is the UCMR 5 timeline?

D. What is the role of “States” in UCMR?

E. How did EPA consider Children's Environmental Health?

F. How did EPA address Environmental Justice?

G. How did EPA coordinate with Indian Tribal Governments?

H. How are laboratories approved for UCMR 5 analyses?

1. Request To Participate

2. Registration

3. Application Package

4. EPA's Review of Application Package

5. Proficiency Testing

6. Written EPA Approval

I. What documents are being incorporated by reference?

1. Methods From the U.S. Environmental Protection Agency

2. Alternative Methods From American Public Health Association—Standard Methods (SM)

3. Methods From ASTM International

IV. Description of Final Rule and Summary of Responses to Public Comments

A. What contaminants must be monitored under UCMR 5?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

a. Aggregate PFAS Measure

b. Legionella Pneumophila

c. Haloacetonitriles

d. 1,2,3-Trichloropropane

B. What is the UCMR 5 sampling design?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

C. What is the sampling frequency and timing?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

D. Where are the sampling locations and what is representative monitoring?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

E. How long do laboratories and PWSs have to report data?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

F. What are the reporting requirements for UCMR 5?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

a. Data Elements

b. Reporting State Data

G. What are the UCMR 5 Minimum Reporting Levels (MRLs) and how were they determined?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

H. What are the requirements for laboratory analysis of field reagent blank samples?

1. This Final Rule

2. Summary of Major Comments and EPA Responses

I. How will EPA support risk communication for UCMR 5 results?

V. Statutory and Executive Order Reviews

A. Executive Order 12866: Regulatory Planning and Review and Executive Order 13563: Improving Regulation and Regulatory Review

B. Paperwork Reduction Act (PRA)

C. Regulatory Flexibility Act (RFA)

D. Unfunded Mandates Reform Act (UMRA)

E. Executive Order 13132: Federalism

F. Executive Order 13175: Consultation and Coordination With Indian Tribal Governments

G. Executive Order 13045: Protection of Children From Environmental Health Risks and Safety Risks

H. Executive Order 13211: Actions Concerning Regulations That Significantly Affect Energy Supply, Distribution or Use

I. National Technology Transfer and Advancement Act (NTTAA)

J. Executive Order 12898: Federal Actions To Address Environmental Justice in Minority Populations and Low-Income Populations

K. Congressional Review Act (CRA)

VI. References

Abbreviations and Acronyms

μg/L Microgram per Liter

11Cl-PF3OUdS 11-chloroeicosafluoro-3-oxaundecane-1-sulfonic Acid

4:2 FTS 1H, 1H, 2H, 2H-perfluorohexane Sulfonic Acid

6:2 FTS 1H, 1H, 2H, 2H-perfluorooctane Sulfonic Acid

8:2 FTS 1H, 1H, 2H, 2H-perfluorodecane Sulfonic Acid

9Cl-PF3ONS 9-chlorohexadecafluoro-3-oxanone-1-sulfonic Acid

ADONA 4,8-dioxa-3H-perfluorononanoic Acid

AES Atomic Emission Spectrometry

ASDWA Association of State Drinking Water Administrators

ASTM ASTM International

AWIA America's Water Infrastructure Act of 2018

CASRN Chemical Abstracts Service Registry Number

CBI Confidential Business Information

CCL Contaminant Candidate List

CCR Consumer Confidence Report

CFR Code of Federal Regulations

CRA Congressional Review Act

CWS Community Water System

DBP Disinfection Byproduct

DWSRF Drinking Water State Revolving Fund

EPA United States Environmental Protection Agency

EPTDS Entry Point to the Distribution System

FR Federal Register

FRB Field Reagent Blank

GW Ground Water

GWRMP Ground Water Representative Monitoring Plan

HFPO-DA Hexafluoropropylene Oxide Dimer Acid (GenX)

HRL Health Reference Level

ICP Inductively Coupled Plasma

ICR Information Collection Request

IDC Initial Demonstration of Capability

LCMRL Lowest Concentration Minimum Reporting Level

LC/MS/MS Liquid Chromatography/Tandem Mass Spectrometry

MDBP Microbial and Disinfection Byproduct

MRL Minimum Reporting Level

NAICS North American Industry Classification System

NCOD National Contaminant Occurrence Database

NDAA National Defense Authorization Act for Fiscal Year 2020

NEtFOSAA N-ethyl Perfluorooctanesulfonamidoacetic Acid

NFDHA Nonafluoro‐3,6‐dioxaheptanoic Acid

ng/L Nanogram per Liter

NMeFOSAA N-methyl Perfluorooctanesulfonamidoacetic Acid

NPDWR National Primary Drinking Water Regulation

NTNCWS Non-transient Non-community Water System

NTTAA National Technology Transfer and Advancement Act

NTWC National Tribal Water Council

OGWDW Office of Ground Water and Drinking Water

OMB Office of Management and Budget

PFAS Per- and Polyfluoroalkyl Substances

PFBA Perfluorobutanoic Acid

PFBS Perfluorobutanesulfonic Acid

PFDA Perfluorodecanoic Acid

PFDoA Perfluorododecanoic Acid

PFEESA Perfluoro (2‐ethoxyethane) Sulfonic Acid

PFHpA Perfluoroheptanoic Acid

PFHpS Perfluoroheptanesulfonic Acid

PFHxA Perfluorohexanoic Acid

PFHxS Perfluorohexanesulfonic Acid

PFMBA Perfluoro‐4‐methoxybutanoic Acid

PFMPA Perfluoro‐3‐methoxypropanoic Acid

PFNA Perfluorononanoic Acid

PFOA Perfluorooctanoic Acid

PFOS Perfluorooctanesulfonic Acid

PFPeA Perfluoropentanoic Acid

PFPeS Perfluoropentanesulfonic Acid

PFTA Perfluorotetradecanoic Acid

PFTrDA Perfluorotridecanoic Acid

PFUnA Perfluoroundecanoic Acid

PN Public Notice

PRA Paperwork Reduction Act

PT Proficiency Testing

PWS Public Water System

QC Quality Control

RFA Regulatory Flexibility Act

SBA Small Business Administration

SBREFA Small Business Regulatory Enforcement Fairness Act

SDWA Safe Drinking Water Act

SDWARS Safe Drinking Water Accession and Review System

SDWIS/Fed Safe Drinking Water Information System Federal Reporting Services

SM Standard Methods for the Examination of Water and Wastewater

SOP Standard Operating Procedure

SPE Solid Phase Extraction

SRMD Standards and Risk Management Division

SW Surface Water

SWTR Surface Water Treatment Rule

TNCWS Transient Non-community Water System

TOF Total Organic Fluorine

TOP Total Oxidizable Precursors

UCMR Unregulated Contaminant Monitoring Rule

UMRA Unfunded Mandates Reform Act of 1995

U.S. United States

USEPA United States Environmental Protection Agency

I. Summary Information

A. Purpose of the Regulatory Action

1. What action is EPA taking?

This final rule requires certain public water systems (PWSs), described in section I.A.2 of this preamble, to collect national occurrence data for 29 PFAS and lithium. PFAS and lithium are not currently subject to national primary drinking water regulations, and EPA is requiring collection of data under UCMR 5 to inform EPA regulatory determinations and risk-management decisions. Consistent with EPA's PFAS Strategic Roadmap, UCMR 5 will provide new data critically needed to improve EPA's understanding of the frequency that 29 PFAS (and lithium) are found in the nation's drinking water systems and at what levels. This data will ensure science-based decision-making and help prioritize protection of disadvantaged communities.

2. Does this action apply to me?

This final rule applies to PWSs described in this section. PWSs are systems that provide water for human consumption through pipes, or constructed conveyances, to at least 15 service connections or that regularly serve an average of at least 25 individuals daily at least 60 days out of the year. A community water system (CWS) is a PWS that has at least 15 service connections used by year-round residents or regularly serves at least 25 year-round residents. A non-transient non-community water system (NTNCWS) is a PWS that is not a CWS and that regularly serves at least 25 of the same people over 6 months per year. Under this final rule, all large CWSs and NTNCWSs serving more than 10,000 people are required to monitor. In addition, small CWSs and NTNCWSs serving between 3,300 and 10,000 people are required to monitor (subject to available EPA appropriations and EPA notification of such requirement) as are the PWSs included in a nationally representative sample of CWSs and NTNCWSs serving between 25 and 3,299 people (see “Selection of Nationally Representative Public Water Systems for the Unregulated Contaminant Monitoring Rule: 2021 Update” for a description of the statistical approach for EPA's selection of the nationally representative sample (USEPA, 2021a), available in the UCMR 5 public docket). EPA expects to clarify the monitoring responsibilities for affected small systems by approximately July 1 of each year preceding sample collection, based on the availability of appropriations each year.

As in previous UCMRs, transient non-community water systems (TNCWSs) ( i.e., non-community water systems that do not regularly serve at least 25 of the same people over 6 months per year) are not required to monitor under UCMR 5. EPA leads UCMR 5 monitoring as a direct-implementation program. States, Territories, and Tribes with primary enforcement responsibility (primacy) to administer the regulatory program for PWSs under SDWA (hereinafter collectively referred to in this document as “states”), can participate in the implementation of UCMR 5 through voluntary Partnership Agreements (see discussion of Partnership Agreements in Section III.D of this preamble). Under Partnership Agreements, states can choose to be involved in various aspects of UCMR 5 monitoring for PWSs they oversee; however, the PWS remains responsible for compliance with the final rule. Potentially regulated categories and entities are identified in the following table.

CategoryExamples of potentially regulated entitiesNAICS *
* NAICS = North American Industry Classification System.
State, local, & Tribal governmentsState, local, and Tribal governments that analyze water samples on behalf of PWSs required to conduct such analysis; State, local, and Tribal governments that directly operate CWSs and NTNCWSs required to monitor924110
Industry