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['Drug and Alcohol Testing - DOT']
['Medical review officer (MRO) - Motor Carrier', 'Substance abuse professional (SAP) - Motor Carrier', 'Drug and alcohol policy - Motor Carrier', 'Alcohol testing - Motor Carrier', 'Split specimen - Motor Carrier', 'Drug testing - Motor Carrier']
03/11/2025
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InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Split specimen - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierEnglishAnalysisFocus AreaUSA
Drug testing
['Drug and Alcohol Testing - DOT']

- Drug testing must be conducted and documented according to DOT regulations.
- Test results determine what actions will follow.
For drug testing:
- A trained collector at a compliant collection site collects a urine or oral-fluid sample from the driver using an approved collection kit. In some cases, the driver must be observed while providing a urine specimen.
- The collector splits the specimen into two bottles, completes a portion of the Department of Transportation (DOT) drug testing chain-of-custody form, and sends the samples to an approved laboratory for testing.
- The laboratory tests the sample and provides the test results to the carrier’s selected medical review officer (MRO).
- The MRO reviews and confirms the test results. If the result is negative, the required paperwork is completed and the employer is notified. If the result is not negative:
- The MRO contacts the driver to discuss the results.
- In some cases, the driver is given the option to have the second (split) specimen tested. If the driver elects (within 72 hours) to have this second test, the split specimen is sent to another laboratory for testing.
- When the results are confirmed, the employer is notified.
- Drivers with a confirmed positive drug test result must be removed from duty and referred to a substance abuse professional (SAP) for treatment. Once the treatment process is complete, drivers may return to driving after passing a return-to-duty test, and then become subject to follow-up testing.
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drug-and-alcohol-testing-dot
FOUNDATIONAL LEARNING
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaCompliance and Exceptions (Level 2)USA
What regulations apply to DOT drug and alcohol testing?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTTransportationIn Depth (Level 3)EnglishFleet SafetyBreath alcohol technician - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisFocus AreaUSA
Key definitions
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Consortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaUSA
What is the designated employer representative’s (DER’s) role?
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaDesignated employer representative (DER) - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierEnglishAnalysisIn Depth Sub Topics (Level 4)USA
DER as contact person
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierFocus AreaUSAAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
What service providers will a motor carrier need to manage its testing program?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTEnglishFleet SafetyBreath alcohol technician - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Auditing service providers
InstituteDrug and alcohol policy - Motor CarrierEnglishFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Consortium/Third-party administrators
InstituteNon-evidential alcohol screening devices - Motor CarrierDrug and alcohol policy - Motor CarrierEvidential breath testing device (EBT) - Motor CarrierFleet SafetyEnglishIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierBreath alcohol technician - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
On-site audits of collection sites
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Providing collectors with necessary information
Drug testing
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Split specimen - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierEnglishAnalysisFocus AreaUSA
['Drug and Alcohol Testing - DOT']

- Drug testing must be conducted and documented according to DOT regulations.
- Test results determine what actions will follow.
For drug testing:
- A trained collector at a compliant collection site collects a urine or oral-fluid sample from the driver using an approved collection kit. In some cases, the driver must be observed while providing a urine specimen.
- The collector splits the specimen into two bottles, completes a portion of the Department of Transportation (DOT) drug testing chain-of-custody form, and sends the samples to an approved laboratory for testing.
- The laboratory tests the sample and provides the test results to the carrier’s selected medical review officer (MRO).
- The MRO reviews and confirms the test results. If the result is negative, the required paperwork is completed and the employer is notified. If the result is not negative:
- The MRO contacts the driver to discuss the results.
- In some cases, the driver is given the option to have the second (split) specimen tested. If the driver elects (within 72 hours) to have this second test, the split specimen is sent to another laboratory for testing.
- When the results are confirmed, the employer is notified.
- Drivers with a confirmed positive drug test result must be removed from duty and referred to a substance abuse professional (SAP) for treatment. Once the treatment process is complete, drivers may return to driving after passing a return-to-duty test, and then become subject to follow-up testing.
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