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['Drug and Alcohol Testing - DOT']
['Drug and alcohol policy - Motor Carrier', 'Alcohol testing - Motor Carrier', 'Drug testing - Motor Carrier']
03/11/2025
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InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaEnglishAnalysisIn Depth Sub Topics (Level 4)USA
Drug-testing labs
['Drug and Alcohol Testing - DOT']

- To be compliant with DOT regulations, labs must be certified by the HHS for drug testing.
Employers may only use laboratories certified by the Department of Health and Human Services (HHS) for drug testing. Each month an agency within HHS, the Substance Abuse and Mental Health Services Administration (SAMHSA), publishes its list of certified laboratories in the Federal Register. Certified laboratories must follow the procedures in Subpart F of Part 40 when conducting DOT drug tests.
Labs may be certified to process urine and/or oral-fluid (saliva) specimens for DOT drug testing.
Employers may not use labs outside of the HHS list and may not process specimens using on-site testing methods for immediate results. Drug testing specimens must be collected, shipped, processed, and results reviewed in accordance with Part 40 procedures.
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drug-and-alcohol-testing-dot
FOUNDATIONAL LEARNING
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaCompliance and Exceptions (Level 2)USA
What regulations apply to DOT drug and alcohol testing?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTTransportationIn Depth (Level 3)EnglishFleet SafetyBreath alcohol technician - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisFocus AreaUSA
Key definitions
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Consortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaUSA
What is the designated employer representative’s (DER’s) role?
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaDesignated employer representative (DER) - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierEnglishAnalysisIn Depth Sub Topics (Level 4)USA
DER as contact person
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierFocus AreaUSAAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
What service providers will a motor carrier need to manage its testing program?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTEnglishFleet SafetyBreath alcohol technician - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Auditing service providers
InstituteDrug and alcohol policy - Motor CarrierEnglishFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Consortium/Third-party administrators
InstituteNon-evidential alcohol screening devices - Motor CarrierDrug and alcohol policy - Motor CarrierEvidential breath testing device (EBT) - Motor CarrierFleet SafetyEnglishIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierBreath alcohol technician - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
On-site audits of collection sites
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Providing collectors with necessary information

- To be compliant with DOT regulations, labs must be certified by the HHS for drug testing.
Employers may only use laboratories certified by the Department of Health and Human Services (HHS) for drug testing. Each month an agency within HHS, the Substance Abuse and Mental Health Services Administration (SAMHSA), publishes its list of certified laboratories in the Federal Register. Certified laboratories must follow the procedures in Subpart F of Part 40 when conducting DOT drug tests.
Labs may be certified to process urine and/or oral-fluid (saliva) specimens for DOT drug testing.
Employers may not use labs outside of the HHS list and may not process specimens using on-site testing methods for immediate results. Drug testing specimens must be collected, shipped, processed, and results reviewed in accordance with Part 40 procedures.
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