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['Drug and Alcohol Testing - DOT']
['Alcohol testing - Motor Carrier', 'Medical review officer (MRO) - Motor Carrier', 'Drug and alcohol policy - Motor Carrier', 'Refusal to be tested - Motor Carrier', 'Designated employer representative (DER) - Motor Carrier', 'Split specimen - Motor Carrier', 'Drug testing - Motor Carrier', 'Dilute specimen - motor carrer']
02/16/2026
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InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierAnalysisIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTSplit specimen - Motor CarrierEnglishFleet SafetyFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierDilute specimen - motor carrerRefusal to be tested - Motor CarrierTransportationUSA
Steps in the MRO’s verification process
['Drug and Alcohol Testing - DOT']

- The MRO must follow specific DOT procedures when discussing non-negative test results with a driver.
When the Medical Review Officer (MRO) has to speak with the driver about a non-negative drug test result, the MRO has specific protocols to follow before being allowed to make a determination with or without speaking with the driver.
Steps to make contact with the driver
Interestingly, the driver is given six opportunities to speak with the MRO as the verification process plays out.
Step 1.
The MRO calls the driver using the phone numbers on the DOT testing form, making three attempts in a 24-hour period.
- If unable to make contact, the MRO documents the attempts and goes to Step 2.
- If able to make contact, the MRO speaks with the driver about a possible medical explanation for a positive, adulterated, substituted, or invalid result.
Step 2.
The MRO tells the company’s designated employer representative (DER) to contact the driver with instructions to immediately contact the MRO.
Step 3.
DER calls the driver using the phone numbers on the DOT testing form making three attempts in a 24-hour period.
- If unsuccessful, the DER must leave a message for the driver and may place the driver in a medically unqualified status or on medical leave.
- If successful, the DER must notify the MRO of the date and time contact was made and inform the driver that the driver has no longer than 72 hours to respond or face a positive or refusal to test.
What happens during the driver interview?
The MRO must explain certain things to the driver during the conversation, including the test results and the verification process. The driver must be told that any additional medical evaluation that is requested must be pursued — or it is considered a refusal to test.
For a claim of a legally obtained prescription, the MRO will take all reasonable and necessary steps to verify the authenticity of all medical records the employee provides and even contact the employee’s personal physician or pharmacist. The driver is also told that information will be provided to third parties without the need for consent in accordance with 40.327. If the MRO calls the driver’s fitness for duty into question, the driver will be given the opportunity to change any legally prescribed medication within five days. In other words, the MRO will not contact the third parties right away.
Also, during the MRO’s conversation with the driver, the MRO must communicate the split specimen option and procedures. The employee has 72 hours from the time the MRO provides this notification to request a test of the split specimen. The employee is given instructions on how to contact the MRO to make the request, including phone numbers or other information that will allow the employee to make this request. The MRO must have the ability to receive the employee’s calls at all times during the 72-hour period, which can include an answering machine or voicemail with a time stamp feature.
The MRO must also tell the employee that the employer cannot deny a request made within the 72 hours. In addition, the driver must be told that the employer cannot require payment for the test before the test takes place, but the employer can seek reimbursement later.
However, the employer who receives a verified positive drug test result must immediately remove the employee involved from performing safety-sensitive functions. The employer must take this action upon receiving the initial report of the verified positive test result. They can’t wait to receive the written report or the result of a split specimen test.
The MRO will also inform the employee that additional tests on the specimen are prohibited. For example, a driver who claims the specimen belongs to someone else cannot request DNA tests.
What happens if the driver and MRO don’t connect?
There are three circumstances in which the MRO may verify a result without an interview with the employee, including:
- If the employee expressly declines the opportunity to discuss the test with the MRO.
- If the DER has successfully made a contact and instructed the employee to contact the MRO and more than 72 hours have passed.
- If neither the MRO nor the DER, after making and documenting all reasonable efforts, has been able to contact the employee within ten days of the date on which the MRO receives the confirmed test result from the laboratory.
After the MRO has verified a test result and reported the result to the DER, the MRO must allow the employee — within 60 days of the verification — to present documentation that serious illness, injury, or other circumstances unavoidably precluded contact with the MRO and/or DER in the times provided. On the basis of such information, the MRO may reopen the verification, allowing the employee to present information concerning whether there is a legitimate medical explanation of the confirmed test result.
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drug-and-alcohol-testing-dot
FOUNDATIONAL LEARNING
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaCompliance and Exceptions (Level 2)USA
What regulations apply to DOT drug and alcohol testing?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTTransportationIn Depth (Level 3)EnglishFleet SafetyBreath alcohol technician - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisFocus AreaUSA
Key definitions
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Consortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaUSA
What is the designated employer representative’s (DER’s) role?
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaDesignated employer representative (DER) - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierEnglishAnalysisIn Depth Sub Topics (Level 4)USA
DER as contact person
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierFocus AreaUSAAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
What service providers will a motor carrier need to manage its testing program?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTEnglishFleet SafetyBreath alcohol technician - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Auditing service providers
InstituteDrug and alcohol policy - Motor CarrierEnglishFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Consortium/Third-party administrators
InstituteNon-evidential alcohol screening devices - Motor CarrierDrug and alcohol policy - Motor CarrierEvidential breath testing device (EBT) - Motor CarrierFleet SafetyEnglishIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierBreath alcohol technician - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
On-site audits of collection sites
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Providing collectors with necessary information
Steps in the MRO’s verification process
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierAnalysisIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTSplit specimen - Motor CarrierEnglishFleet SafetyFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierDilute specimen - motor carrerRefusal to be tested - Motor CarrierTransportationUSA
['Drug and Alcohol Testing - DOT']

- The MRO must follow specific DOT procedures when discussing non-negative test results with a driver.
When the Medical Review Officer (MRO) has to speak with the driver about a non-negative drug test result, the MRO has specific protocols to follow before being allowed to make a determination with or without speaking with the driver.
Steps to make contact with the driver
Interestingly, the driver is given six opportunities to speak with the MRO as the verification process plays out.
Step 1.
The MRO calls the driver using the phone numbers on the DOT testing form, making three attempts in a 24-hour period.
- If unable to make contact, the MRO documents the attempts and goes to Step 2.
- If able to make contact, the MRO speaks with the driver about a possible medical explanation for a positive, adulterated, substituted, or invalid result.
Step 2.
The MRO tells the company’s designated employer representative (DER) to contact the driver with instructions to immediately contact the MRO.
Step 3.
DER calls the driver using the phone numbers on the DOT testing form making three attempts in a 24-hour period.
- If unsuccessful, the DER must leave a message for the driver and may place the driver in a medically unqualified status or on medical leave.
- If successful, the DER must notify the MRO of the date and time contact was made and inform the driver that the driver has no longer than 72 hours to respond or face a positive or refusal to test.
What happens during the driver interview?
The MRO must explain certain things to the driver during the conversation, including the test results and the verification process. The driver must be told that any additional medical evaluation that is requested must be pursued — or it is considered a refusal to test.
For a claim of a legally obtained prescription, the MRO will take all reasonable and necessary steps to verify the authenticity of all medical records the employee provides and even contact the employee’s personal physician or pharmacist. The driver is also told that information will be provided to third parties without the need for consent in accordance with 40.327. If the MRO calls the driver’s fitness for duty into question, the driver will be given the opportunity to change any legally prescribed medication within five days. In other words, the MRO will not contact the third parties right away.
Also, during the MRO’s conversation with the driver, the MRO must communicate the split specimen option and procedures. The employee has 72 hours from the time the MRO provides this notification to request a test of the split specimen. The employee is given instructions on how to contact the MRO to make the request, including phone numbers or other information that will allow the employee to make this request. The MRO must have the ability to receive the employee’s calls at all times during the 72-hour period, which can include an answering machine or voicemail with a time stamp feature.
The MRO must also tell the employee that the employer cannot deny a request made within the 72 hours. In addition, the driver must be told that the employer cannot require payment for the test before the test takes place, but the employer can seek reimbursement later.
However, the employer who receives a verified positive drug test result must immediately remove the employee involved from performing safety-sensitive functions. The employer must take this action upon receiving the initial report of the verified positive test result. They can’t wait to receive the written report or the result of a split specimen test.
The MRO will also inform the employee that additional tests on the specimen are prohibited. For example, a driver who claims the specimen belongs to someone else cannot request DNA tests.
What happens if the driver and MRO don’t connect?
There are three circumstances in which the MRO may verify a result without an interview with the employee, including:
- If the employee expressly declines the opportunity to discuss the test with the MRO.
- If the DER has successfully made a contact and instructed the employee to contact the MRO and more than 72 hours have passed.
- If neither the MRO nor the DER, after making and documenting all reasonable efforts, has been able to contact the employee within ten days of the date on which the MRO receives the confirmed test result from the laboratory.
After the MRO has verified a test result and reported the result to the DER, the MRO must allow the employee — within 60 days of the verification — to present documentation that serious illness, injury, or other circumstances unavoidably precluded contact with the MRO and/or DER in the times provided. On the basis of such information, the MRO may reopen the verification, allowing the employee to present information concerning whether there is a legitimate medical explanation of the confirmed test result.
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