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['Drug and Alcohol Testing - DOT']
['Alcohol testing - Motor Carrier', 'Medical review officer (MRO) - Motor Carrier', 'Drug and alcohol policy - Motor Carrier', 'Drug testing - Motor Carrier']
02/16/2026
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InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
Medical review officers
['Drug and Alcohol Testing - DOT']

- Medical review officers confirm test results, check the lab’s performance, and check the chain-of-custody for employers.
- Companies should only use licensed and trained physicians as MROs.
The medical review officer’s (MRO) responsibility is a significant function within the testing process. The MRO’s job is to provide a “clinical confirmation” of a presumptive positive as reported by the laboratory.
Essentially, a positive test is initially a scientific conclusion which then must be legitimized by medical considerations made by the MRO. The MRO then determines whether a true positive exists. The laboratory reports the result directly to the MRO for a professional review of the results which will only be reported to the company after a “clinical confirmation” has been achieved by the MRO.
This is likely to be the most critical decision employers make within a testing program. The MRO will have more contact with the company than anyone else within the program. The person will become the company’s “agent” providing final analysis of the total process and representing the company in any challenge litigation that may evolve.
MRO chief duties include:
- Verify results, both negative and positive;
- Check the lab’s performance; and
- Check the chain-of-custody.
Seek out only licensed physicians (Doctor of Medicine or osteopaths). MROs must meet the knowledge, training, and education requirements described in 40.121. There are professional bodies which provide certification such as AAMRO (American Association of Medical Review Officers) and MROCC (Medical Review Officer Certification Council). This certification guarantees a knowledgeable and competent MD; it is the assurance a company should demand from a qualified professional. The websites of these organizations include searchable databases of certified MROs to help employers find an acceptable one:
:
drug-and-alcohol-testing-dot
FOUNDATIONAL LEARNING
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaCompliance and Exceptions (Level 2)USA
What regulations apply to DOT drug and alcohol testing?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTTransportationIn Depth (Level 3)EnglishFleet SafetyBreath alcohol technician - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisFocus AreaUSA
Key definitions
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Consortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaUSA
What is the designated employer representative’s (DER’s) role?
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaDesignated employer representative (DER) - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierEnglishAnalysisIn Depth Sub Topics (Level 4)USA
DER as contact person
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierFocus AreaUSAAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
What service providers will a motor carrier need to manage its testing program?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierIn Depth Sub Topics (Level 4)Drug testing - Motor CarrierDrug and Alcohol Testing - DOTEnglishFleet SafetyBreath alcohol technician - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Auditing service providers
InstituteDrug and alcohol policy - Motor CarrierEnglishFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Consortium/Third-party administrators
InstituteNon-evidential alcohol screening devices - Motor CarrierDrug and alcohol policy - Motor CarrierEvidential breath testing device (EBT) - Motor CarrierFleet SafetyEnglishIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierBreath alcohol technician - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
On-site audits of collection sites
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Providing collectors with necessary information
Medical review officers
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
['Drug and Alcohol Testing - DOT']

- Medical review officers confirm test results, check the lab’s performance, and check the chain-of-custody for employers.
- Companies should only use licensed and trained physicians as MROs.
The medical review officer’s (MRO) responsibility is a significant function within the testing process. The MRO’s job is to provide a “clinical confirmation” of a presumptive positive as reported by the laboratory.
Essentially, a positive test is initially a scientific conclusion which then must be legitimized by medical considerations made by the MRO. The MRO then determines whether a true positive exists. The laboratory reports the result directly to the MRO for a professional review of the results which will only be reported to the company after a “clinical confirmation” has been achieved by the MRO.
This is likely to be the most critical decision employers make within a testing program. The MRO will have more contact with the company than anyone else within the program. The person will become the company’s “agent” providing final analysis of the total process and representing the company in any challenge litigation that may evolve.
MRO chief duties include:
- Verify results, both negative and positive;
- Check the lab’s performance; and
- Check the chain-of-custody.
Seek out only licensed physicians (Doctor of Medicine or osteopaths). MROs must meet the knowledge, training, and education requirements described in 40.121. There are professional bodies which provide certification such as AAMRO (American Association of Medical Review Officers) and MROCC (Medical Review Officer Certification Council). This certification guarantees a knowledgeable and competent MD; it is the assurance a company should demand from a qualified professional. The websites of these organizations include searchable databases of certified MROs to help employers find an acceptable one:
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