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['Drug and Alcohol Testing - DOT']
['Alcohol testing - Motor Carrier', 'Drug and alcohol policy - Motor Carrier', 'Follow-up alcohol and drug testing - Motor Carrier', 'Safety-sensitive function - Motor Carrier', 'Random alcohol and drug testing- Motor Carrier', 'Return-to-duty drug and alcohol testing - Motor Carrier', 'Drug testing - Motor Carrier', 'Substance abuse professional (SAP) - Motor Carrier']
07/29/2025
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InstituteDrug and alcohol policy - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTReturn-to-duty drug and alcohol testing - Motor CarrierUSAEnglishFleet SafetyRandom alcohol and drug testing- Motor CarrierFocus AreaSubstance abuse professional (SAP) - Motor CarrierSafety-sensitive function - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationFollow-up alcohol and drug testing - Motor CarrierIn Depth (Level 3)
Follow-up tests
['Drug and Alcohol Testing - DOT']

- At least six unannounced follow-up tests are required for drivers who return-to-duty following treatment for a drug or alcohol violation.
- Follow-up testing can last up to 60 months.
- Follow-up drug tests must be performed under direct observation.
Unannounced follow-up tests, numbering at least six in the 12 months following the driver’s return to duty, are required after evaluation, treatment, and negative return-to-duty testing, using the procedures in Part 40. A substance abuse professional (SAP) may also require follow-up tests during the 48 months of safety-sensitive duty following this first 12-month period.
Follow-up drug tests must be conducted under direct observation. In the event a follow-up drug test is not performed under direct observation, the test will not be canceled since failure to observe is not a fatal flaw (see 40.209). However, the Federal Motor Carrier Safety Administration expects carriers to perform another test to be compliant.
Follow-up testing is separate from and in addition to the regular random testing program. Drivers subject to follow-up testing must remain in the standard random pool and must be tested whenever the individual’s name comes up for random testing, even if this means being tested twice in the same day, week, or month.
Follow-up testing for alcohol can be performed only when the driver is performing safety-sensitive functions, or immediately prior to performing or immediately after performing safety-sensitive functions.
Employers must report to the Drug and Alcohol Clearinghouse when a driver completes a follow-up testing plan under the carrier’s Part 382 program. The employer doesn’t report each individual follow-up test.
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drug-and-alcohol-testing-dot
FOUNDATIONAL LEARNING
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaCompliance and Exceptions (Level 2)USA
What regulations apply to DOT drug and alcohol testing?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTUSAEnglishFleet SafetyBreath alcohol technician - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
Key definitions
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationIn Depth (Level 3)Consortium: third-party administrator (TPA) - Motor CarrierDesignated employer representative (DER) - Motor CarrierSafety-sensitive function - Motor CarrierEnglishAnalysisFocus AreaUSA
What is the designated employer representative’s (DER’s) role?
InstituteDrug and alcohol policy - Motor CarrierAlcohol testing - Motor CarrierMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierTransportationFocus AreaDesignated employer representative (DER) - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierEnglishAnalysisIn Depth Sub Topics (Level 4)USA
DER as contact person
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyDrug and Alcohol Testing - DOTDrug testing - Motor CarrierConsortium: third-party administrator (TPA) - Motor CarrierFocus AreaUSAAlcohol testing - Motor CarrierAnalysisTransportationIn Depth (Level 3)
What service providers will a motor carrier need to manage its testing program?
InstituteDrug and alcohol policy - Motor CarrierMedical review officer (MRO) - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTTransportationEnglishFleet SafetyBreath alcohol technician - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierSubstance abuse professional (SAP) - Motor CarrierAlcohol testing - Motor CarrierAnalysisIn Depth Sub Topics (Level 4)USA
Auditing service providers
InstituteDrug and alcohol policy - Motor CarrierEnglishFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaConsortium: third-party administrator (TPA) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Consortium/Third-party administrators
InstituteNon-evidential alcohol screening devices - Motor CarrierDrug and alcohol policy - Motor CarrierEvidential breath testing device (EBT) - Motor CarrierFleet SafetyEnglishIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierBreath alcohol technician - Motor CarrierFocus AreaAlcohol testing - Motor CarrierAnalysisTransportationUSA
On-site audits of collection sites
InstituteDrug and alcohol policy - Motor CarrierEnglishMedical review officer (MRO) - Motor CarrierFleet SafetyIn Depth Sub Topics (Level 4)Drug and Alcohol Testing - DOTDrug testing - Motor CarrierFocus AreaDesignated employer representative (DER) - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationUSA
Providing collectors with necessary information
Follow-up tests
InstituteDrug and alcohol policy - Motor CarrierDrug testing - Motor CarrierDrug and Alcohol Testing - DOTReturn-to-duty drug and alcohol testing - Motor CarrierUSAEnglishFleet SafetyRandom alcohol and drug testing- Motor CarrierFocus AreaSubstance abuse professional (SAP) - Motor CarrierSafety-sensitive function - Motor CarrierAlcohol testing - Motor CarrierAnalysisTransportationFollow-up alcohol and drug testing - Motor CarrierIn Depth (Level 3)
['Drug and Alcohol Testing - DOT']

- At least six unannounced follow-up tests are required for drivers who return-to-duty following treatment for a drug or alcohol violation.
- Follow-up testing can last up to 60 months.
- Follow-up drug tests must be performed under direct observation.
Unannounced follow-up tests, numbering at least six in the 12 months following the driver’s return to duty, are required after evaluation, treatment, and negative return-to-duty testing, using the procedures in Part 40. A substance abuse professional (SAP) may also require follow-up tests during the 48 months of safety-sensitive duty following this first 12-month period.
Follow-up drug tests must be conducted under direct observation. In the event a follow-up drug test is not performed under direct observation, the test will not be canceled since failure to observe is not a fatal flaw (see 40.209). However, the Federal Motor Carrier Safety Administration expects carriers to perform another test to be compliant.
Follow-up testing is separate from and in addition to the regular random testing program. Drivers subject to follow-up testing must remain in the standard random pool and must be tested whenever the individual’s name comes up for random testing, even if this means being tested twice in the same day, week, or month.
Follow-up testing for alcohol can be performed only when the driver is performing safety-sensitive functions, or immediately prior to performing or immediately after performing safety-sensitive functions.
Employers must report to the Drug and Alcohol Clearinghouse when a driver completes a follow-up testing plan under the carrier’s Part 382 program. The employer doesn’t report each individual follow-up test.
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