[The federally–designated routes on the National Network consist of the Interstate System, except as noted, and the following additional Federal–aid Primary highways]
| Route | From | To |
|---|
| US 27 | FL Turnpike Ext | FL 84 Andytown. |
| US 27 | South Bay | I–75 Ocala. |
| US 301 | SR 24 Waldo | I–10. |
| FL 24 | SR 331 Gainesville | US 301 Waldo. |
| FL 85 | FL 397 Valparaiso | I–10 near Crestview. |
| FL 202 | I–95 Jacksonville | FL A–1–A. |
| FL 263 | US 90 W. of Tallahassee | I–10. |
| FL 331 | I–75 S. of Gainesville | FL 24. |
| FL 397 | Entrance Eglin AFB | FL 85 Valparaiso. |
| FL 528 – FL 407 | I–4 Orlando | Cape Canaveral. |
| 20th St. Expwy | 1–95 Jacksonville | Adams St. near Matthews Bridge. |
| FL Turnpike | S. End of Homestead Extension | I–75 Wildwood. |
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