§136.3 Identification of test procedures.

(a) Parameters or pollutants, for which methods are approved, are listed together with test procedure descriptions and references in Tables IA, IB, IC, ID, IE, IF, IG, and IH of this section. The methods listed in Tables IA, IB, IC, ID, IE, IF, IG, and IH are incorporated by reference, see paragraph (b) of this section, with the exception of EPA Methods 200.7, 601-613, 624.1, 625.1, 1613, 1624, and 1625. The full texts of Methods 601-613, 624.1, 625.1, 1613, 1624, and 1625 are printed in appendix A of this part, and the full text of Method 200.7 is printed in appendix C of this part. The full text for determining the method detection limit when using the test procedures is given in appendix B of this part. In the event of a conflict between the reporting requirements of 40 CFR parts 122 and 125 and any reporting requirements associated with the methods listed in these tables, the provisions of 40 CFR parts 122 and 125 are controlling and will determine a permittee's reporting requirements. The full texts of the referenced test procedures are incorporated by reference into Tables IA, IB, IC, ID, IE, IF, IG, and IH. The date after the method number indicates the latest editorial change of the method. The discharge parameter values for which reports are required must be determined by one of the standard analytical test procedures incorporated by reference and described in Tables IA, IB, IC, ID, IE, IF, IG, and IH or by any alternate test procedure which has been approved by the Administrator under the provisions of paragraph (d) of this section and §§136.4 and 136.5. Under certain circumstances paragraph (c) of this section, §136.5(a) through (d) or 40 CFR 401.13, other additional or alternate test procedures may be used.

Parameter and units	Method ¹	EPA	Standard methods	AOAC, ASTM, USGS	Other
Bacteria
1. Coliform (fecal), number per 100 mL or number per gram dry weight	Most Probable Number (MPN), 5 tube, 3 dilution, or	p. 132³, 1680^{11 15}, 1681^{11 20}	9221 E-2014
	Membrane filter (MF) ^{2 5}, single step	p. 124³	9222 D-2015 ²⁹	B-0050-85 ⁴
2. Coliform (fecal), number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 132 ³	9221 E-2014; 9221 F-2014 ³³
	Multiple tube/multiple well, or				Colilert-18®. ^{13 18 28}
	MF ^{2 5}, single step⁵	p. 124 ³	9222 D-2015 ²⁹
3. Coliform (total), number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 114 ³	9221 B-2014
	MF^{2 5} , single step or two step	p. 108 ³	9222 B-2015 ³⁰	B-0025-85 ⁴
	MF ^{2 5}, with enrichment	p. 111 ³	9222 B-2015³⁰
4. E. coli, number per 100 mL	MPN multiple tube, or		9221 B2014/9221 F-2014 ^{12 14 33}
	multiple tube/multiple well, or		9223 B-2016 ¹³	991.15 ¹⁰	Colilert® ^{13 18 28} Colilert-18® ^{13 17 18}
	MF^{2 5 6 7 8}, two step, or		9222 B-2015/9222 I-2015 ³¹
	Single step	1603 ²¹			m-ColiBlue24®. ¹⁹
5. Fecal streptococci, number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 139 ³	9230 B-2013
	MF² , or	p. 136 ³	9230 C-2013³²	B-0055-85 ⁴
	Plate count	p. 143 ³
6. Enterococci, number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 139 ³	9230 B-2013
	MPN ^{6 8}, multiple tube/multiple well, or		9230 D-2013	D6503-99 ⁹	Enterolert®. ^{13 23}
	MF ^{2 5 6 7 8}single step or	1600 ²⁴	9230 C-2013 ³²
	Plate count	p. 143 ³
7. Salmonella, number per gram dry weight ¹¹	MPN multiple tube	1682 ²²
Aquatic Toxicity
8. Toxicity, acute, fresh water organisms, LC₅₀, percent effluent	Water flea, Cladoceran, Ceriodaphnia dubia acute	2002.0 ²⁵
	Water fleas, Cladocerans, Daphnia pulex and Daphnia magna acute	2021.0 ²⁵
	Fish, Fathead minnow, Pimephales promelas, and Bannerfin shiner, Cyprinella leedsi, acute	2000.0 ²⁵
	Fish, Rainbow trout, Oncorhynchus mykiss, and brook trout, Salvelinus fontinalis, acute	2019.0 ²⁵
9. Toxicity, acute, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, LC₅₀, percent effluent	Mysid, Mysidopsis bahia, acute	2007.0 .²⁵
	Fish, Sheepshead minnow, Cyprinodon variegatus, acute	2004.0 .²⁵
	Fish, Silverside, Menidia beryllina, Menidia menidia, and Menidia peninsulae, acute	2006.0 . ²⁵
10. Toxicity, chronic, fresh water organisms, NOEC or IC₂₅, percent effluent	Fish, Fathead minnow, Pimephales promelas, larval survival and growth	1000.0 ²⁶
	Fish, Fathead minnow, Pimephales promelas, embryo-larval survival and teratogenicity	1001.0 ²⁶
	Water flea, Cladoceran, Ceriodaphnia dubia, survival and reproduction	1002.0 ²⁶
	Green alga, Selenastrum capricornutum, growth	1003.0²⁶
11. Toxicity, chronic, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, NOEC or IC₂₅, percent effluent	Fish, Sheepshead minnow, Cyprinodon variegatus, larval survival and growth	1004.0 . ²⁷
	Fish, Sheepshead minnow, Cyprinodon variegatus, embryo-larval survival and teratogenicity	1005.0 ²⁷
	Fish, Inland silverside, Menidia beryllina, larval survival and growth	1006.0 ²⁷
	Mysid, Mysidopsis bahia, survival, growth, and fecundity	1007.0 ²⁷
	Sea urchin, Arbacia punctulata, fertilization	1008.0 ²⁷
Table IA notes: ¹ The method must be specified when results are reported. ² A 0.45-µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of extractables which could interfere with their growth. ³ Microbiological Methods for Monitoring the Environment, Water and Wastes, EPA/600/8-78/017. 1978. U.S. EPA. ⁴ U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS. ⁵ Because the MF technique usually yields low and variable recovery from chlorinated wastewaters, the Most Probable Number method will be required to resolve any controversies. ⁶ Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample. ⁷ When the MF method has been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and comparability of results. ⁸ To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines. ⁹ Annual Book of ASTM Standards-Water and Environmental Technology, Section 11.02. 2000, 1999, 1996. ASTM International. ¹⁰ Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, 1998. AOAC International. ¹¹ Recommended for enumeration of target organism in sewage sludge. ¹² The multiple-tube fermentation test is used in 9221B.2-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis. ¹³ These tests are collectively known as defined enzyme substrate tests. ¹⁴ After prior enrichment in a presumptive medium for total coliform using 9221B.2-2014, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 µg/mL of MUG may be used. ¹⁵ Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using Lauryl-Tryptose Broth (LTB) and EC Medium, EPA-821-R-14-009. September 2014. U.S. EPA. ¹⁶ Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert® may be enumerated with the multiple-well procedures, Quanti-Tray® or Quanti-Tray®/2000 and the MPN calculated from the table provided by the manufacturer. ¹⁷ Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35 °C rather than the 24 h required for the Colilert® test and is recommended for marine water samples. ¹⁸ Descriptions of the Colilert®, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories, Inc. ¹⁹ A description of the mColiBlue24® test is available from Hach Company. ²⁰ Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using A-1 Medium, EPA-821-R-06-013. July 2006. U.S. EPA. ²¹ Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified Membrane-Thermotolerant Escherichia coli Agar (modified mTEC), EPA-821-R-14-010. September 2014. U.S. EPA. ²² Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium, EPA-821-R-14-012. September 2014. U.S. EPA. ²³ A description of the Enterolert® test may be obtained from IDEXX Laboratories Inc. ²⁴ Method 1600: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-14-011. September 2014. U.S. EPA. ²⁵ Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, EPA-821-R-02-012. Fifth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016. ²⁶ Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013. Fourth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016. ²⁷ Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, EPA-821-R-02-014. Third Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016. ²⁸ To use Colilert-18® to assay for fecal coliforms, the incubation temperature is 44.5 ± 0.2 °C, and a water bath incubator is used. ²⁹ On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources. ³⁰ On a monthly basis, at least ten sheen colonies from positive samples must be verified using lauryl tryptose broth and brilliant green lactose bile broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using lauryl tryptose broth. Where possible, verifications should be done from randomized sample sources. ³¹ Subject coliform positive samples determined by 9222 B-2015 or other membrane filter procedure to 9222 I-2015 using NA-MUG media. ³² Verification of colonies by incubation of BHI agar at 10 ± 0.5 °C for 48 ± 3 h is optional. As per the Errata to the 23rd Edition of Standard Methods for the Examination of Water and Wastewater “Growth on a BHI agar plate incubated at 10 ± 0.5 °C for 48 ± 3 h is further verification that the colony belongs to the genus Enterococcus.” ³³ 9221 F.2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted vials collect gas produced by thermotolerant fecal coliforms.

Parameter	Method	EPA ^2 7 10	Standard methods	ASTM	Other
1. Aldrin	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96 (02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
2. Ametryn	GC	507, 619			See footnote,³ p. 83; See footnote,⁹ O-3106-93; See footnote,⁶ p. S68.
	GC/MS	525.2, 625.1			See footnote,¹⁴ O-1121-91.
3. Aminocarb	TLC				See footnote,³ p. 94; See footnote,⁶ p. S60.
	HPLC	632
4. Atraton	GC	619			See footnote,³ p. 83; See footnote,⁶ p. S68.
	GC/MS	625.1
5. Atrazine	GC	507, 619, 608.3			See footnote,³ p. 83; See footnote,⁶ p. S68; See footnote,⁹ O-3106-93.
	HPLC/MS				See footnote,¹² O-2060-01.
	GC/MS	525.1, 525.2, 625.1			See footnote,¹¹ O-1126-95.
6. Azinphos methyl	GC	614, 622, 1657			See footnote,³ p. 25; See footnote,⁶ p. S51.
	GC/MS	625.1			See footnote,¹¹ O-1126-95.
7. Barban	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	GC/MS	625.1
8. α-BHC	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁸ 3M0222.
	GC/MS	625.1 ⁵	6410 B-2000		See footnote,¹¹ O-1126-95.
9. β-BHC	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
10. δ-BHC	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
11. γ-BHC (Lindane)	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1 ⁵	6410 B-2000		See footnote,¹¹ O-1126-95.
12. Captan	GC	617, 608.3	6630 B-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7.
13. Carbaryl	TLC				See footnote,³ p. 94, See footnote,⁶ p. S60.
	HPLC	531.1, 632
	HPLC/MS	553			See footnote,¹² O-2060-01.
	GC/MS	625.1			See footnote,¹¹ O-1126-95.
14. Carbophenothion	GC	617, 608.3	6630 B-2007		See footnote,⁴ page 27; See footnote,⁶ p. S73.
	GC/MS	625.1
15. Chlordane	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
16. Chloropropham	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	GC/MS	625.1
17. 2,4-D	GC	615	6640 B-2006		See footnote,³ p. 115; See footnote,⁴ O-3105-83.
	HPLC/MS				See footnote,¹² O-2060-01.
18. 4,4′-DDD	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3105-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
19. 4,4′-DDE	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000		See footnote,¹¹ O-1126-95.
20. 4,4′-DDT	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
21. Demeton-O	GC	614, 622			See footnote,³ p. 25; See footnote,⁶ p. S51.
	GC/MS	625.1
22. Demeton-S	GC	614, 622			See footnote,³ p. 25; See footnote,⁶ p. S51.
	GC/MS	625.1
23. Diazinon	GC	507, 614, 622, 1657			See footnote,³ p. 25; See footnote,⁴ O-3104-83; See footnote,⁶ p. S51.
	GC/MS	525.2, 625.1			See footnote,¹¹ O-1126-95.
24. Dicamba	GC	615			See footnote,³ p. 115.
	HPLC/MS				See footnote,¹² O-2060-01.
25. Dichlofenthion	GC	622.1			See footnote,⁴ page 27; See footnote,⁶ p. S73.
26. Dichloran	GC	608.2, 617, 608.3	6630 B-2007		See footnote,³ p. 7.
27. Dicofol	GC	617, 608.3			See footnote,⁴ O-3104-83.
28. Dieldrin	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000		See footnote,¹¹ O-1126-95.
29. Dioxathion	GC	614.1, 1657			See footnote,⁴ page 27; See footnote,⁶ p. S73.
30. Disulfoton	GC	507, 614, 622, 1657			See footnote,³ p. 25; See footnote,⁶ p. S51.
	GC/MS	525.2, 625.1			See footnote,¹¹ O-1126-95.
31. Diuron	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS	553			See footnote,¹² O-2060-01.
32. Endosulfan I	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222).
	GC/MS	625.1 ⁵	6410 B-2000		See footnote,¹³ O-2002-01.
33. Endosulfan II	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁸ 3M0222.
	GC/MS	625.1 ⁵	6410 B-2000		See footnote,¹³ O-2002-01.
34. Endosulfan Sulfate	GC	617, 608.3	6630 C-2007		See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
35. Endrin	GC	505, 508, 617, 1656, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	525.1, 525.2, 625.1⁵	6410 B-2000
36. Endrin aldehyde	GC	617, 608.3	6630 C-2007		See footnote,⁸ 3M0222.
	GC/MS	625.1
37. Ethion	GC	614, 614.1, 1657			See footnote,⁴ page 27; See footnote,⁶ p. S73.
	GC/MS	625.1			See footnote,¹³ O-2002-01.
38. Fenuron	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS				See footnote,¹² O-2060-01.
39. Fenuron-TCA	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
40. Heptachlor	GC	505, 508, 617, 1656, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	525.1, 525.2, 625.1	6410 B-2000
41. Heptachlor epoxide	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁶ p. S73; See footnote,⁸ 3M0222.
	GC/MS	625.1	6410 B-2000
42. Isodrin	GC	617, 608.3	6630 B-2007 & C-2007		See footnote,⁴ O-3104-83; See footnote,⁶ p. S73.
	GC/MS	625.1
43. Linuron	GC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS	553			See footnote,¹² O-2060-01.
	GC/MS				See footnote,¹¹ O-1126-95.
44. Malathion	GC	614, 1657	6630 B-2007		See footnote,³ p. 25; See footnote,⁶ p. S51.
	GC/MS	625.1			See footnote,¹¹ O-1126-95.
45. Methiocarb	TLC				See footnote,³ p. 94; See footnote,⁶ p. S60.
	HPLC	632
	HPLC/MS				See footnote,¹² O-2060-01.
46. Methoxychlor	GC	505, 508, 608.2, 617, 1656, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83; See footnote,⁸ 3M0222.
	GC/MS	525.1, 525.2, 625.1			See footnote,¹¹ O-1126-95.
47. Mexacarbate	TLC				See footnote,³ p. 94; See footnote,⁶ p. S60.
	HPLC	632
	GC/MS	625.1
48. Mirex	GC	617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote,⁴ O-3104-83.
	GC/MS	625.1
49. Monuron	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
50. Monuron-TCA	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
51. Neburon	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS				See footnote,¹² O-2060-01.
52. Parathion methyl	GC	614, 622, 1657	6630 B-2007		See footnote,⁴ page 27; See footnote,³ p. 25.
	GC/MS	625.1			See footnote,¹¹ O-1126-95.
53. Parathion ethyl	GC	614	6630 B-2007		See footnote,⁴ page 27; See footnote,³ p. 25.
	GC/MS				See footnote,¹¹ O-1126-95.
54. PCNB	GC	608.1, 617, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7.
55. Perthane	GC	617, 608.3		D3086-90, D5812-96(02)	See footnote,⁴ O-3104-83.
56. Prometon	GC	507, 619			See footnote,³ p. 83; See footnote,⁶ p. S68; See footnote,⁹ O-3106-93.
	GC/MS	525.2, 625.1			See footnote,¹¹ O-1126-95.
57. Prometryn	GC	507, 619			See footnote,³ p. 83; See footnote,⁶ p. S68; See footnote,⁹ O-3106-93.
	GC/MS	525.1, 525.2, 625.1			See footnote,¹³ O-2002-01.
58. Propazine	GC	507, 619, 1656, 608.3			See footnote,³ p. 83; See footnote,⁶ p. S68; See footnote,⁹ O-3106-93.
	GC/MS	525.1, 525.2, 625.1
59. Propham	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS				See footnote,¹² O-2060-01.
60. Propoxur	TLC				See footnote,³ p. 94; See footnote,⁶ p. S60.
	HPLC	632
61. Secbumeton	TLC				See footnote,³ p. 83; See footnote,⁶ p. S68.
	GC	619
62. Siduron	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
	HPLC/MS				See footnote,¹² O-2060-01.
63. Simazine	GC	505, 507, 619, 1656, 608.3			See footnote,³ p. 83; See footnote,⁶ p. S68; See footnote,⁹ O-3106-93.
	GC/MS	525.1, 525.2, 625.1			See footnote,¹¹ O-1126-95.
64. Strobane	GC	617, 608.3	6630 B-2007 & C-2007		See footnote,³ p. 7.
65. Swep	TLC				See footnote,³ p. 104; See footnote,⁶ p. S64.
	HPLC	632
66. 2,4,5-T	GC	615	6640 B-2006		See footnote,³ p. 115; See footnote,⁴ O-3105-83.
67. 2,4,5-TP (Silvex)	GC	615	6640 B-2006		See footnote,³ p. 115; See footnote,⁴ O-3105-83.
68. Terbuthylazine	GC	619, 1656, 608.3			See footnote,³ p. 83; See footnote,⁶ p. S68.
	GC/MS				See footnote,¹³ O-2002-01.
69. Toxaphene	GC	505, 508, 617, 1656, 608.3	6630 B-2007 & C-2007	D3086-90, D5812-96(02)	See footnote,³ p. 7; See footnote; ⁸ See footnote,⁴ O-3105-83.
	GC/MS	525.1, 525.2, 625.1	6410 B-2000
70. Trifluralin	GC	508, 617, 627, 1656, 608.3	6630 B-2007		See footnote,³ p. 7; See footnote,⁹ O-3106-93.
	GC/MS	525.2, 625.1			See footnote,¹¹ O-1126-95.
Table ID notes:
¹ Pesticides are listed in this table by common name for the convenience of the reader. Additional pesticides may be found under Table IC of this section, where entries are listed by chemical name.
² The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at appendix B of this part, Definition and Procedure for the Determination of the Method Detection Limit.
³ Methods for Benzidine, Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA. This EPA publication includes thin-layer chromatography (TLC) methods.
⁴ Methods for the Determination of Organic Substances in Water and Fluvial Sediments, Techniques of Water-Resources Investigations of the U.S. Geological Survey, Book 5, Chapter A3. 1987. USGS.
⁵ The method may be extended to include α-BHC, γ-BHC, endosulfan I, endosulfan II, and endrin. However, when they are known to exist, Method 608.3 is the preferred method.
⁶ Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).
⁷ Each analyst must make an initial, one-time, demonstration of their ability to generate acceptable precision and accuracy with Methods 608.3 and 625.1 in accordance with procedures given in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis, must spike and analyze 5% of all samples analyzed with Method 608.3 or 5% of all samples analyzed with Method 625.1 to monitor and evaluate laboratory data quality in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the warning limits, the analytical results for that parameter in the unspiked sample are suspect. The results should be reported, but cannot be used to demonstrate regulatory compliance. These quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.
⁸ Organochlorine Pesticides and PCBs in Wastewater Using Empore^TM Disk. Revised October 28, 1994. 3M Corporation.
⁹ Method O-3106-93 is in Open File Report 94-37, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Triazine and Other Nitrogen-Containing Compounds by Gas Chromatography With Nitrogen Phosphorus Detectors. 1994. USGS.
¹⁰ EPA Methods 608.1, 608.2, 614, 614.1, 615, 617, 619, 622, 622.1, 627, and 632 are found in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, EPA 821-R-92-002, April 1992, U.S. EPA. EPA Methods 505, 507, 508, 525.1, 531.1 and 553 are in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume II, EPA 821-R-93-010B, 1993, U.S. EPA. EPA Method 525.2 is in Determination of Organic Compounds in Drinking Water by Liquid-Solid Extraction and Capillary Column Gas Chromatography/Mass Spectrometry, Revision 2.0, 1995, U.S. EPA. EPA methods 1656 and 1657 are in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume I, EPA 821-R-93-010A, 1993, U.S. EPA. Methods 608.3 and 625.1 are available at https://www.epa.gov/cwa-methods/approved-cwa-test-methods-organic-compounds.
¹¹ Method O-1126-95 is in Open-File Report 95-181, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion monitoring. 1995. USGS.
¹² Method O-2060-01 is in Water-Resources Investigations Report 01-4134, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of Pesticides in Water by Graphitized Carbon-Based Solid-Phase Extraction and High-Performance Liquid Chromatography/Mass Spectrometry. 2001. USGS.
¹³ Method O-2002-01 is in Water-Resources Investigations Report 01-4098, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of moderate-use pesticides in water by C-18 solid-phase extraction and capillary-column gas chromatography/mass spectrometry. 2001. USGS.
¹⁴ Method O-1121-91 is in Open-File Report 91-519, Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of organonitrogen herbicides in water by solid-phase extraction and capillary-column gas chromatography/mass spectrometry with selected-ion monitoring. 1992. USGS.

Parameter and units	Method	Reference (method number or page)
Parameter and units	Method	EPA¹	Standard Methods 18th, 19th, 20th Ed.	Standard Methods Online	ASTM	USGS²
1. Alpha-Total, pCi per liter	Proportional or scintillation counter	900.0	7110 B	7110 B-00	D1943-90, 96	pp. 75 and 78³
2. Alpha-Counting error, pCi per liter	Proportional or scintillation counter	Appendix B	7110 B	7110 B-00	D1943-90, 96	p. 79
3. Beta-Total, pCi per liter	Proportional counter	900.0	7110 B	7110 B-00	D1890-90, 96	pp. 75 and 78³
4. Beta-Counting error, pCi	Proportional counter	Appendix B	7110 B	7110 B-00	D1890-90, 96	p. 79
5. (a) Radium Total pCi per liter (b) Ra, pCi per liter	Proportional counter	903.0	7500-Ra B	7500-Ra B-01	D2460-90, 97
	Scintillation counter	903.1	7500-Ra C	7500-Ra C-01	D3454-91, 97	p. 81

Pharmaceuticals pollutants	CAS registry No.	Analytical method number
Acetonitrile	75-05-8	1666/1671/D3371/D3695/624.1
n-Amyl acetate	628-63-7	1666/D3695
n-Amyl alcohol	71-41-0	1666/D3695
Benzene	71-43-2	D4763/D3695/502.2/524.2/624.1
n-Butyl-acetate	123-86-4	1666/D3695
tert-Butyl alcohol	75-65-0	1666/624.1
Chlorobenzene	108-90-7	502.2/524.2/624.1
Chloroform	67-66-3	502.2/524.2/551/624.1
o-Dichlorobenzene	95-50-1	1625C/502.2/524.2/624.1
1,2-Dichloroethane	107-06-2	D3695/502.2/524.2/624.1
Diethylamine	109-89-7	1666/1671
Dimethyl sulfoxide	67-68-5	1666/1671
Ethanol	64-17-5	1666/1671/D3695/624.1
Ethyl acetate	141-78-6	1666/D3695/624.1
n-Heptane	142-82-5	1666/D3695
n-Hexane	110-54-3	1666/D3695
Isobutyraldehyde	78-84-2	1666/1667
Isopropanol	67-63-0	1666/D3695
Isopropyl acetate	108-21-4	1666/D3695
Isopropyl ether	108-20-3	1666/D3695
Methanol	67-56-1	1666/1671/D3695/624.1
Methyl Cellosolve® (2-Methoxy ethanol)	109-86-4	1666/1671
Methylene chloride	75-09-2	502.2/524.2/624.1
Methyl formate	107-31-3	1666
4-Methyl-2-pentanone (MIBK)	108-10-1	1624C/1666/D3695/D4763/524.2/624.1
Phenol	108-95-2	D4763
n-Propanol	71-23-8	1666/1671/D3695/624.1
2-Propanone (Acetone)	67-64-1	D3695/D4763/524.2/624.1
Tetrahydrofuran	109-99-9	1666/524.2/624.1
Toluene	108-88-3	D3695/D4763/502.2/524.2/624.1
Triethlyamine	121-44-8	1666/1671
Xylenes	(Note 1)	1624C/1666/624.1
Table IF note:
¹ 1624C: m-xylene 108-38-3, o,p-xylene, E-14095 (Not a CAS number; this is the number provided in the Environmental Monitoring Methods Index [EMMI] database.); 1666: m,p-xylene 136777-61-2, o-xylene 95-47-6.

EPA survey code	Pesticide name	CAS No.	EPA analytical method No.(s) ³
8	Triadimefon	43121-43-3	507/633/525.1/525.2/1656/625.1.
12	Dichlorvos	62-73-7	1657/507/622/525.1/525.2/625.1.
16	2,4-D; 2,4-D Salts and Esters [2,4-Dichloro-phenoxyacetic acid]	94-75-7	1658/515.1/615/515.2/555.
17	2,4-DB; 2,4-DB Salts and Esters [2,4-Dichlorophenoxybutyric acid]	94-82-6	1658/515.1/615/515.2/555.
22	Mevinphos	7786-34-7	1657/507/622/525.1/525.2/625.1.
25	Cyanazine	21725-46-2	629/507/608.3/625.1.
26	Propachlor	1918-16-7	1656/508/608.1/525.1/525.2/608.3/625.1.
27	MCPA; MCPA Salts and Esters [2-Methyl-4-chlorophenoxyacetic acid]	94-74-6	1658/615/555.
30	Dichlorprop; Dichlorprop Salts and Esters [2-(2,4-Dichlorophenoxy) propionic acid]	120-36-5	1658/515.1/615/515.2/555.
31	MCPP; MCPP Salts and Esters [2-(2-Methyl-4-chlorophenoxy) propionic acid]	93-65-2	1658/615/555.
35	TCMTB [2-(Thiocyanomethylthio) benzo-thiazole]	21564-17-0	637.
39	Pronamide	23950-58-5	525.1/525.2/507/633.1/625.1.
41	Propanil	709-98-8	632.1/1656/608.3.
45	Metribuzin	21087-64-9	507/633/525.1/525.2/1656/608.3/625.1.
52	Acephate	30560-19-1	1656/1657/608.3.
53	Acifluorfen	50594-66-6	515.1/515.2/555.
54	Alachlor	15972-60-8	505/507/645/525.1/525.2/1656/608.3/625.1.
55	Aldicarb	116-06-3	531.1.
58	Ametryn	834-12-8	507/619/525.2/625.1.
60	Atrazine	1912-24-9	505/507/619/525.1/525.2/1656/ 608.3/625.1.
62	Benomyl	17804-35-2	631.
68	Bromacil; Bromacil Salts and Esters	314-40-9	507/633/525.1/525.2/1656/608.3/625.1.
69	Bromoxynil	1689-84-5	1625/1661/625.1.
69	Bromoxynil Octanoate	1689-99-2	1656/608.3.
70	Butachlor	23184-66-9	507/645/525.1/525.2/1656/608.3/625.1.
73	Captafol	2425-06-1	1656/608.3/625.1.
75	Carbaryl [Sevin]	63-25-2	531.1/632/553/625.1.
76	Carbofuran	1563-66-2	531.1/632/625.1.
80	Chloroneb	2675-77-6	1656/508/608.1/525.1/525.2/608.3/625.1.
82	Chlorothalonil	1897-45-6	508/608.2/525.1/525.2/1656/608.3/625.1.
84	Stirofos	961-11-5	1657/507/622/525.1/525.2/625.1.
86	Chlorpyrifos	2921-88-2	1657/508/622/625.1.
90	Fenvalerate	51630-58-1	1660.
103	Diazinon	333-41-5	1657/507/614/622/525.2/625.1.
107	Parathion methyl	298-00-0	1657/614/622/625.1.
110	DCPA [Dimethyl 2,3,5,6-tetrachloro-terephthalate]	1861-32-1	508/608.2/525.1/525.2/515.1 ²/515.2 ²/1656/608.3/625.1.
112	Dinoseb	88-85-7	1658/515.1/615/515.2/555/625.1.
113	Dioxathion	78-34-2	1657/614.1.
118	Nabonate [Disodium cyanodithio-imidocarbonate]	138-93-2	630.1.
119	Diuron	330-54-1	632/553.
123	Endothall	145-73-3	548/548.1.
124	Endrin	72-20-8	1656/505/508/617/525.1/525.2/608.3/625.1.
125	Ethalfluralin	55283-68-6	1656/627/608.3 See footnote 1.
126	Ethion	563-12-2	1657/614/614.1/625.1.
127	Ethoprop	13194-48-4	1657/507/622/525.1/525.2/625.1.
132	Fenarimol	60168-88-9	507/633.1/525.1/525.2/1656/608.3/625.1.
133	Fenthion	55-38-9	1657/622/625.1.
138	Glyphosate [N-(Phosphonomethyl) glycine]	1071-83-6	547.
140	Heptachlor	76-44-8	1656/505/508/617/525.1/525.2/608.3/625.1.
144	Isopropalin	33820-53-0	1656/627/608.3.
148	Linuron	330-55-2	553/632.
150	Malathion	121-75-5	1657/614/625.1.
154	Methamidophos	10265-92-6	1657.
156	Methomyl	16752-77-5	531.1/632.
158	Methoxychlor	72-43-5	1656/505/508/608.2/617/525.1/525.2/608.3/625.1.
172	Nabam	142-59-6	630/630.1.
173	Naled	300-76-5	1657/622/625.1.
175	Norflurazon	27314-13-2	507/645/525.1/525.2/1656/608.3/625.1.
178	Benfluralin	1861-40-1	1656/627/608.3 See footnote 1.
182	Fensulfothion	115-90-2	1657/622/625.1.
183	Disulfoton	298-04-4	1657/507/614/622/525.2/625.1.
185	Phosmet	732-11-6	1657/622.1/625.1.
186	Azinphos Methyl	86-50-0	1657/614/622/625.1.
192	Organo-tin pesticides	12379-54-3	Ind-01/200.7/200.9.
197	Bolstar	35400-43-2	1657/622.
203	Parathion	56-38-2	1657/614/625.1.
204	Pendimethalin	40487-42-1	1656.
205	Pentachloronitrobenzene	82-68-8	1656/608.1/617/608.3/625.1.
206	Pentachlorophenol	87-86-5	1625/515.2/555/515.1/525.1/525.2/625.1.
208	Permethrin	52645-53-1	608.2/508/525.1/525.2/1656/1660/608.3 ⁴/625.1 ⁴.
212	Phorate	298-02-2	1657/622/625.1.
218	Busan 85 [Potassium dimethyldithiocarbamate]	128-03-0	630/630.1.
219	Busan 40 [Potassium N-hydroxymethyl-N-methyldithiocarbamate]	51026-28-9	630/630.1.
220	KN Methyl [Potassium N-methyl-dithiocarbamate]	137-41-7	630/630.1.
223	Prometon	1610-18-0	507/619/525.2/625.1.
224	Prometryn	7287-19-6	507/619/525.1/525.2/625.1.
226	Propazine	139-40-2	507/619/525.1/525.2/1656/608.3/625.1.
230	Pyrethrin I	121-21-1	1660.
232	Pyrethrin II	121-29-9	1660.
236	DEF [S,S,S-Tributyl phosphorotrithioate]	78-48-8	1657.
239	Simazine	122-34-9	505/507/619/525.1/525.2/1656/608.3/625.1.
241	Carbam-S [Sodium dimethyldithio-carbamate]	128-04-1	630/630.1.
243	Vapam [Sodium methyldithiocarbamate]	137-42-8	630/630.1.
252	Tebuthiuron	34014-18-1	507/525.1/525.2/625.1.
254	Terbacil	5902-51-2	507/633/525.1/525.2/1656/608.3/625.1.
255	Terbufos	13071-79-9	1657/507/614.1/525.1/525.2/625.1.
256	Terbuthylazine	5915-41-3	619/1656/608.3.
257	Terbutryn	886-50-0	507/619/525.1/525.2/625.1.
259	Dazomet	533-74-4	630/630.1/1659.
262	Toxaphene	8001-35-2	1656/505/508/617/525.1/525.2/608.3/625.1.
263	Merphos [Tributyl phosphorotrithioate]	150-50-5	1657/507/525.1/525.2/622/625.1.
264	Trifluralin ¹	1582-09-8	1656/508/617/627/525.2/608.3/625.1.
268	Ziram [Zinc dimethyldithiocarbamate]	137-30-4	630/630.1.
Table IG notes:
¹ Monitor and report as total Trifluralin.
² Applicable to the analysis of DCPA degradates.
³ EPA Methods 608.1 through 645, 1645 through 1661, and Ind-01 are available in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume I, EPA 821-R-93-010A, Revision I, August 1993, U.S. EPA. EPA Methods 200.9 and 505 through 555 are available in Methods for the Determination of Nonconventional Pesticides in Municipal and Industrial Wastewater, Volume II, EPA 821-R-93-010B, August 1993, U.S. EPA. The full text of Methods 608.3, 625.1, and 1625 are provided at appendix A of this part. The full text of Method 200.7 is provided at appendix C of this part. Methods 608.3 and 625.1 are available at https://www.epa.gov/cwa-methods/approved-cwa-test-methods-organic-compounds.
⁴ Permethrin is not listed within methods 608.3 and 625.1; however, cis-permethrin and trans-permethrin are listed. Permethrin can be calculated by adding the results of cis- and trans-permethrin.

Parameter ¹	Method	EPA ² ⁷	Standard methods	ASTM	Other
1. Acenaphthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
2. Acenaphthylene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
3. Acrolein	GC	603
	GC/MS	624.1,⁴ 1624B
4. Acrylonitrile	GC	603
	GC/MS	624.1,⁴ 1624B			O-4127-96. ¹³
5. Anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
6. Benzene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
7. Benzidine	Spectrophoto- metric				See footnote ³, p.1.
	GC/MS	625.1,⁵ 1625B	6410 B-2000
	HPLC	605
8. Benzo(a)anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
9. Benzo(a)pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
10. Benzo(b)fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
11. Benzo(g,h,i)perylene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
12. Benzo(k)fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
13. Benzyl chloride	GC				See footnote ³, p. 130.
	GC/MS				See footnote ⁶, p. S102.
14. Butyl benzyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
15. bis(2-Chloroethoxy) methane	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
16. bis(2-Chloroethyl) ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
17. bis(2-Ethylhexyl) phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
18. Bromodichloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
19. Bromoform	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
20. Bromomethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
21. 4-Bromophenyl phenyl ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
22. Carbon tetrachloride	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
23. 4-Chloro-3-methyl phenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
24. Chlorobenzene	GC	601, 602	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
25. Chloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96.¹³
26. 2-Chloroethylvinyl ether	GC	601
	GC/MS	624.1, 1624B
27. Chloroform	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
28. Chloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
29. 2-Chloronaphthalene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
30. 2-Chlorophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
31. 4-Chlorophenyl phenyl ether	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
32. Chrysene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
33. Dibenzo(a,h)anthracene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
34. Dibromochloromethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
35. 1,2-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote, ⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
36. 1,3-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote ⁹, p. 27; O-4127-96.¹³
37. 1,4-Dichlorobenzene	GC	601, 602	6200 C-2011
	GC/MS	624.1, 1625B	6200 B-2011		See footnote,⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
38. 3,3′-Dichlorobenzidine	GC/MS	625.1, 1625B	6410 B-2000
	HPLC	605
39. Dichlorodifluoromethane	GC	601
	GC/MS		6200 C-2011		O-4127-96 ¹³, O-4436-16.¹⁴
40. 1,1-Dichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
41. 1,2-Dichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
42. 1,1-Dichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
43. trans-1,2-Dichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
44. 2,4-Dichlorophenol	GC	604	6420 B-2000.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
45. 1,2-Dichloropropane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
46. cis-1,3-Dichloropropene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
47. trans-1,3-Dichloropropene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
48. Diethyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
49. 2,4-Dimethylphenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
50. Dimethyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
51. Di-n-butyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹, p. 27.
52. Di-n-octyl phthalate	GC	606
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27.
53. 2, 4-Dinitrophenol	GC	604	6420 B-2000		See footnote ⁹, p. 27.
	GC/MS	625.1, 1625B	6410 B-2000
54. 2,4-Dinitrotoluene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
55. 2,6-Dinitrotoluene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
56. Epichlorohydrin	GC				See footnote ³, p. 130.
	GC/MS				See footnote ⁶, p. S102.
57. Ethylbenzene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96,¹³ O-4436-16.¹⁴
58. Fluoranthene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
59. Fluorene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
60. 1,2,3,4,6,7,8-Heptachloro-dibenzofuran	GC/MS	1613B
61. 1,2,3,4,7,8,9-Heptachloro-dibenzofuran	GC/MS	1613B
62. 1,2,3,4,6,7,8-Heptachloro-dibenzo-p-dioxin	GC/MS	1613B
63. Hexachlorobenzene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
64. Hexachlorobutadiene	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96.¹³
65. Hexachlorocyclopentadiene	GC	612
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96.¹³
66. 1,2,3,4,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
67. 1,2,3,6,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
68. 1,2,3,7,8,9-Hexachloro-dibenzofuran	GC/MS	1613B
69. 2,3,4,6,7,8-Hexachloro-dibenzofuran	GC/MS	1613B
70. 1,2,3,4,7,8-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
71. 1,2,3,6,7,8-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
72. 1,2,3,7,8,9-Hexachloro-dibenzo-p-dioxin	GC/MS	1613B
73. Hexachloroethane	GC	612
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27; O-4127-96.¹³
74. Indeno(1,2,3-c,d) pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
75. Isophorone	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
76. Methylene chloride	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
77. 2-Methyl-4,6-dinitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
78. Naphthalene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005
79. Nitrobenzene	GC	609
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC			D4657-92 (98)
80. 2-Nitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
81. 4-Nitrophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
82. N-Nitrosodimethylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
83. N-Nitrosodi-n-propylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
84. N-Nitrosodiphenylamine	GC	607
	GC/MS	625.1,⁵ 1625B	6410 B-2000		See footnote,⁹ p. 27.
85. Octachlorodibenzofuran	GC/MS	1613B.¹⁰
86. Octachlorodibenzo-p-dioxin	GC/MS	1613B.¹⁰
87. 2,2′-oxybis(1-chloropropane) ¹² [also known as bis(2-Chloro-1-methylethyl) ether]	GC	611
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
88. PCB-1016	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
89. PCB-1221	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
90. PCB-1232	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
91. PCB-1242	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
92. PCB-1248	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
93. PCB-1254	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
94. PCB-1260	GC	608.3			See footnote ³, p. 43; See footnote.⁸
	GC/MS	625.1	6410 B-2000
95. 1,2,3,7,8-Pentachloro-dibenzofuran	GC/MS	1613B
96. 2,3,4,7,8-Pentachloro-dibenzofuran	GC/MS	1613B
97. 1,2,3,7,8-Pentachloro-dibenzo-p-dioxin	GC/MS	1613B
98. Pentachlorophenol	GC	604	6420 B-2000		See footnote ³, p. 140.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
99. Phenanthrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
100. Phenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
101. Pyrene	GC	610
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
	HPLC	610	6440 B-2005	D4657-92 (98)
102. 2,3,7,8-Tetrachloro-dibenzofuran	GC/MS	1613B.¹⁰
103. 2,3,7,8-Tetrachloro-dibenzo-p-dioxin	GC/MS	613, 625.1,^5a 1613B
104. 1,1,2,2-Tetrachloroethane	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96.¹³
105. Tetrachloroethene	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
106. Toluene	GC	602	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
107. 1,2,4-Trichlorobenzene	GC	612			See footnote ³, p. 130.
	GC/MS	625.1, 1625B	6410 B-2000		See footnote,⁹ p. 27; O-4127-96,¹³ O-4436-16.¹⁴
108. 1,1,1-Trichloroethane	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
109. 1,1,2-Trichloroethane	GC	601	6200 C-2011		See footnote ³, p. 130.
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
110. Trichloroethene	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
111. Trichlorofluoromethane	GC	601	6200 C-2011
	GC/MS	624.1	6200 B-2011		O-4127-96.¹³
112. 2,4,6-Trichlorophenol	GC	604	6420 B-2000
	GC/MS	625.1, 1625B	6410 B-2000		See footnote ⁹, p. 27.
113. Vinyl chloride	GC	601	6200 C-2011
	GC/MS	624.1, 1624B	6200 B-2011		O-4127-96 ¹³, O-4436-16.¹⁴
114. Nonylphenol	GC/MS			D7065-17
115. Bisphenol A (BPA)	GC/MS			D7065-17
116. p-tert-Octylphenol (OP)	GC/MS			D7065-17
117. Nonylphenol Monoethoxylate (NP1EO)	GC/MS			D7065-17
118. Nonylphenol Diethoxylate (NP2EO)	GC/MS			D7065-17
119. Adsorbable Organic Halides (AOX)	Adsorption and Coulometric Titration	1650.¹¹
120. Chlorinated Phenolics	In Situ Acetylation and GC/MS	1653.¹¹
Table IC notes:
¹ All parameters are expressed in micrograms per liter (µg/L) except for Method 1613B, in which the parameters are expressed in picograms per liter (pg/L).
² The full text of Methods 601-613, 1613B, 1624B, and 1625B are provided at appendix A, Test Procedures for Analysis of Organic Pollutants. The standardized test procedure to be used to determine the method detection limit (MDL) for these test procedures is given at appendix B of this part, Definition and Procedure for the Determination of the Method Detection Limit. These methods are available at: https://www.epa.gov/cwa-methods as individual PDF files.
³ Methods for Benzidine: Chlorinated Organic Compounds, Pentachlorophenol and Pesticides in Water and Wastewater. September 1978. U.S. EPA.
⁴ Method 624.1 may be used for quantitative determination of acrolein and acrylonitrile, provided that the laboratory has documentation to substantiate the ability to detect and quantify these analytes at levels necessary to comply with any associated regulations. In addition, the use of sample introduction techniques other than simple purge-and-trap may be required. QC acceptance criteria from Method 603 should be used when analyzing samples for acrolein and acrylonitrile in the absence of such criteria in Method 624.1.
⁵ Method 625.1 may be extended to include benzidine, hexachlorocyclopentadiene, N-nitrosodimethylamine, N-nitrosodi-n-propylamine, and N-nitrosodiphenylamine. However, when they are known to be present, Methods 605, 607, and 612, or Method 1625B, are preferred methods for these compounds.
^5a Method 625.1 screening only.
⁶ Selected Analytical Methods Approved and Cited by the United States Environmental Protection Agency, Supplement to the 15th Edition of Standard Methods for the Examination of Water and Wastewater. 1981. American Public Health Association (APHA).
⁷ Each analyst must make an initial, one-time demonstration of their ability to generate acceptable precision and accuracy with Methods 601-603, 1624B, and 1625B in accordance with procedures in Section 8.2 of each of these methods. Additionally, each laboratory, on an on-going basis must spike and analyze 10% (5% for Methods 624.1 and 625.1 and 100% for methods 1624B and 1625B) of all samples to monitor and evaluate laboratory data quality in accordance with Sections 8.3 and 8.4 of these methods. When the recovery of any parameter falls outside the quality control (QC) acceptance criteria in the pertinent method, analytical results for that parameter in the unspiked sample are suspect. The results should be reported but cannot be used to demonstrate regulatory compliance. If the method does not contain QC acceptance criteria, control limits of ± three standard deviations around the mean of a minimum of five replicate measurements must be used. These quality control requirements also apply to the Standard Methods, ASTM Methods, and other methods cited.
⁸ Organochlorine Pesticides and PCBs in Wastewater Using Empore^TM Disk. Revised October 28, 1994. 3M Corporation.
⁹ Method O-3116-87 is in Open File Report 93-125, Methods of Analysis by U.S. Geological Survey National Water Quality Laboratory—Determination of Inorganic and Organic Constituents in Water and Fluvial Sediments. 1993. USGS.
¹⁰ Analysts may use Fluid Management Systems, Inc. Power-Prep system in place of manual cleanup provided the analyst meets the requirements of Method 1613B (as specified in Section 9 of the method) and permitting authorities. Method 1613, Revision B, Tetra- through Octa-Chlorinated Dioxins and Furans by Isotope Dilution HRGC/HRMS. Revision B, 1994. U.S. EPA. The full text of this method is provided in appendix A to this part and at https://www.epa.gov/cwa-methods/approved-cwa-test-methods-organic-compounds.
¹¹ Method 1650, Adsorbable Organic Halides by Adsorption and Coulometric Titration. Revision C, 1997 U.S. EPA. Method 1653, Chlorinated Phenolics in Wastewater by In Situ Acetylation and GCMS. Revision A, 1997 U.S. EPA. The full text for both of these methods is provided at appendix A in part 430 of this chapter, The Pulp, Paper, and Paperboard Point Source Category.
¹² The compound was formerly inaccurately labeled as 2,2′-oxybis(2-chloropropane) and bis(2-chloroisopropyl) ether. Some versions of Methods 611, and 1625 inaccurately list the analyte as “bis(2-chloroisopropyl)ether,” but use the correct CAS number of 108-60-1.
¹³ Method O-4127-96, U.S. Geological Survey Open-File Report 97-829, Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory—Determination of 86 volatile organic compounds in water by gas chromatography/mass spectrometry, including detections less than reporting limits,1998, USGS.
¹⁴ Method O-4436-16 U.S. Geological Survey Techniques and Methods, book 5, chap. B12, Determination of heat purgeable and ambient purgeable volatile organic compounds in water by gas chromatography/mass spectrometry, 2016, USGS.

Parameter and units	Method ¹	EPA	Standard methods	AOAC, ASTM, USGS	Other
Bacteria
1. Coliform (fecal), number per 100 mL	Most Probable Number (MPN), 5 tube, 3 dilution, or	p. 132 ³	9221 E-2014, 9221 F-2014 ³²
	Membrane filter (MF)² , single step	p. 124 ³	9222 D-2015 ²⁶	B-0050-85 ⁴
2. Coliform (total), number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 114 ³	9221 B-2014	B-0025-85 ⁴
	MF ², single step or two step	p. 108 ³	9222 B-2015 ²⁷
	MF² with enrichment	p. 111 ³	9222 B- 2015 ²⁷
3. E. coli, number per 100 mL	MPN^{5 7 13} , multiple tube, or		9221 B.3-2014/9221 F-2014^{10 12 32}
	Multiple tube/multiple well, or		9223 B-2016 ¹¹	991.15⁹	Colilert® ^{11 15}, Colilert-18®. ^{11 14 15}
	MF ^{2 5 6 7}, two step, or	1103.1 ¹⁸	9222 B-2015/9222 I-2015¹⁷, 9213 D-2007	D5392-93 ⁸
	Single step	1603¹⁹, 1604 ²⁰			m-ColiBlue24® ¹⁶, KwikCount EC. ^{28 29}
4. Fecal streptococci, number per 100 mL	MPN, 5 tube, 3 dilution, or	p. 139³	9230 B-2013	B-0055-85⁴
	MF² , or	p. 136 ¹⁸	9230 C-2013 ³⁰
	Plate count	p. 143 ¹⁸
5. Enterococci, number per 100 mL	MPN^{5 7}, multiple tube/multiple well, or		9230 D-2013	D6503-99⁸	Enterolert®. ^{11 21}
	MF^{2 5 6 7} two step, or	1106.1²²	9230 C-2013 ³⁰	D5259-92 ⁸
	Single step, or	1600 ²⁰³	9230 C-2013 ³⁰
	Plate count	p. 143¹⁸
Protozoa
6. Cryptosporidium	Filtration/IMS/FA	1622 ²⁴, 1623²⁵ , 1623.1^{25 31}
7. Giardia	Filtration/IMS/FA	1623 ²⁵, 1623.1 ^{25 31}
Table 1H notes: ¹ The method must be specified when results are reported. ² A 0.45-µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of extractables which could interfere with their growth. ³ Microbiological Methods for Monitoring the Environment, Water and Wastes. EPA/600/8-78/017. 1978. US EPA. ⁴ U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS. ⁵ Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample. ⁶ When the MF method has not been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and comparability of results. ⁷ To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines. ⁸ Annual Book of ASTM Standards—Water and Environmental Technology. Section 11.02. 2000, 1999, 1996. ASTM International. ⁹ Official Methods of Analysis of AOAC International, 16th Edition, Volume I, Chapter 17. 1995. AOAC International. ¹⁰ The multiple-tube fermentation test is used in 9221B.3-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis. ¹¹ These tests are collectively known as defined enzyme substrate tests. ¹² After prior enrichment in a presumptive medium for total coliform using 9221B.3-2014, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 µg/mL of MUG may be used. ¹³ Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert® may be enumerated with the multiple-well procedures, Quanti-Tray® or Quanti-Tray®/2000, and the MPN calculated from the table provided by the manufacturer. ¹⁴ Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35 °C, rather than the 24 h required for the Colilert® test, and is recommended for marine water samples. ¹⁵ Descriptions of the Colilert®, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories Inc. ¹⁶ A description of the mColiBlue24® test may be obtained from Hach Company. ¹⁷ Subject coliform positive samples determined by 9222B-2015 or other membrane filter procedure to 9222I-2015 using NA-MUG media. ¹⁸ Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC), EPA-821-R-10-002. March 2010. US EPA. ¹⁹ Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-14-010. September 2014. US EPA. ²⁰ Method 1604: Total Coliforms and Escherichia coli (E. coli) in Water by Membrane Filtration by Using a Simultaneous Detection Technique (MI Medium), EPA 821-R-02-024. September 2002. US EPA. ²¹ A description of the Enterolert® test may be obtained from IDEXX Laboratories Inc. ²² Method 1106.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA), EPA-821-R-09-015. December 2009. US EPA. ²³ Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-14-011. September 2014. US EPA. ²⁴ Method 1622 uses a filtration, concentration, immunomagnetic separation of oocysts from captured material, immunofluorescence assay to determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the detection of Cryptosporidium. Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, EPA-821-R-05-001. December 2005. US EPA. ²⁵ Methods 1623 and 1623.1 use a filtration, concentration, immunomagnetic separation of oocysts and cysts from captured material, immunofluorescence assay to determine concentrations, and confirmation through vital dye staining and differential interference contrast microscopy for the simultaneous detection of Cryptosporidium and Giardia oocysts and cysts. Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA-821-R-05-002. December 2005. US EPA. Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA 816-R-12-001. January 2012. US EPA. ²⁶ On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources. ²⁷ On a monthly basis, at least ten sheen colonies from positive samples must be verified using Lauryl Tryptose Broth and brilliant green lactose bile broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources. ²⁸ A description of KwikCount EC may be obtained from Micrology Laboratories LLC. ²⁹ Approved for the analyses of E. coli in freshwater only. ³⁰ Verification of colonies by incubation of BHI agar at 10 ± 0.5 °C for 48 ± 3 h is optional. As per the Errata to the 23rd Edition of Standard Methods for the Examination of Water and Wastewater “Growth on a BHI agar plate incubated at 10 ± 0.5 °C for 48 ± 3 h is further verification that the colony belongs to the genus Enterococcus.” ³¹ Method 1623.1 includes updated acceptance criteria for IPR, OPR, and MS/MSD and clarifications and revisions based on the use of Method 1623 for years and technical support questions. ³² 9221 F.2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted vials collect gas produced by thermotolerant fecal coliforms.

Parameter number/name	Container ¹	Preservation ^{2 3}	Maximum holding time ⁴
Table IA—Bacterial Tests
1-4. Coliform, total, fecal, and E. coli	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃⁵	8 hours. ^{22 23}
5. Fecal streptococci	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours. ²²
6. Enterococci	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours. ²²
7. Salmonella	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours. ²²
Table IA—Aquatic Toxicity Tests
8-11. Toxicity, acute and chronic	P, FP, G	Cool, ≤6°C ¹⁶	36 hours.
Table IB—Inorganic Tests
1. Acidity	P, FP, G	Cool, ≤6°C ¹⁸	14 days.
2. Alkalinity	P, FP, G	Cool, ≤6°C ¹⁸	14 days.
4. Ammonia	P, FP, G	Cool, ≤6°C¹⁸ , H₂SO₄ to pH <2	28 days.
9. Biochemical oxygen demand	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
10. Boron	P, FP, or Quartz	HNO₃ to pH <2	6 months.
11. Bromide	P, FP, G	None required	28 days.
14. Biochemical oxygen demand, carbonaceous	P, FP G	Cool, ≤6°C ¹⁸	48 hours.
15. Chemical oxygen demand	P, FP, G	Cool, ≤6°C¹⁸, H₂SO₄ to pH <2	28 days.
16. Chloride	P, FP, G	None required	28 days.
17. Chlorine, total residual	P, G	None required	Analyze within 15 minutes.
21. Color	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
23-24. Cyanide, total or available (or CATC) and free	P, FP, G	Cool, ≤6°C¹⁸ , NaOH to pH >10^{5 6} , reducing agent if oxidizer present	14 days.
25. Fluoride	P	None required	28 days.
27. Hardness	P, FP, G	HNO₃ or H₂SO₄ to pH <2	6 months.
28. Hydrogen ion (pH)	P, FP, G	None required	Analyze within 15 minutes.
31, 43. Kjeldahl and organic N	P, FP, G	Cool, ≤6°C¹⁸, H₂SO₄ to pH <2	28 days.
Table IB—Metals⁷
18. Chromium VI	P, FP, G	Cool, ≤6°C¹⁸, pH = 9.3-9.7 ²⁰	28 days.
35. Mercury (CVAA)	P, FP, G	HNO₃ to pH <2	28 days.
35. Mercury (CVAFS)	FP, G; and FP-lined cap ¹⁷	5 mL/L 12N HCl or 5 mL/L BrCl ¹⁷	90 days. ¹⁷
3, 5-8, 12, 13, 19, 20, 22, 26, 29, 30, 32-34, 36, 37, 45, 47, 51, 52, 58-60, 62, 63, 70-72, 74, 75. Metals, except boron, chromium VI, and mercury	P, FP, G	HNO₃ to pH <2, or at least 24 hours prior to analysis ¹⁹	6 months.
38. Nitrate	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
39. Nitrate-nitrite	P, FP, G	Cool, ≤6°C ¹⁸, H₂SO₄ to pH <2	28 days.
40. Nitrite	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
41. Oil and grease	G	Cool to ≤6°C ¹⁸, HCl or H₂SO₄ to pH <2	28 days.
42. Organic Carbon	P, FP, G	Cool to ≤6°C¹⁸, HCl, H₂SO₄, or H₃PO₄ to pH <2	28 days.
44. Orthophosphate	P, FP, G	Cool, to ≤6°C ^{18 24}	Filter within 15 minutes; Analyze within 48 hours.
46. Oxygen, Dissolved Probe	G, Bottle and top	None required	Analyze within 15 minutes.
47. Winkler	G, Bottle and top	Fix on site and store in dark	8 hours.
48. Phenols	G	Cool, ≤6°C ¹⁸, H₂SO₄ to pH <2	28 days.
49. Phosphorus (elemental)	G	Cool, ≤6°C ¹⁸	48 hours.
50. Phosphorus, total	P, FP, G	Cool, ≤6°C ¹⁸, H₂SO₄ to pH <2	28 days.
53. Residue, total	P, FP, G	Cool, ≤6°C ¹⁸	7 days.
54. Residue, Filterable (TDS)	P, FP, G	Cool, ≤6°C ¹⁸	7 days.
55. Residue, Nonfilterable (TSS)	P, FP, G	Cool, ≤6°C ¹⁸	7 days.
56. Residue, Settleable	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
57. Residue, Volatile	P, FP, G	Cool, ≤6°C ¹⁸	7 days.
61. Silica	P or Quartz	Cool, ≤6°C ¹⁸	28 days.
64. Specific conductance	P, FP, G	Cool, ≤6°C ¹⁸	28 days.
65. Sulfate	P, FP, G	Cool, ≤6°C ¹⁸	28 days.
66. Sulfide	P, FP, G	Cool, ≤6°C ¹⁸, add zinc acetate plus sodium hydroxide to pH >9	7 days.
67. Sulfite	P, FP, G	None required	Analyze within 15 minutes.
68. Surfactants	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
69. Temperature	P, FP, G	None required	Analyze within 15 minutes.
73. Turbidity	P, FP, G	Cool, ≤6°C ¹⁸	48 hours.
Table IC—Organic Tests⁸
13, 18-20, 22, 24, 25, 27, 28, 34-37, 39-43, 45-47, 56, 76, 104, 105, 108-111, 113. Purgeable Halocarbons	G, FP-lined septum	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃ ⁵, HCl to pH 2 ⁹	14 days. ⁹
26. 2-Chloroethylvinyl ether	G, FP-lined septum	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃⁵	14 days.
6, 57, 106. Purgeable aromatic hydrocarbons	G, FP-lined septum	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃ ⁵, HCl to pH 2 ⁹	14 days. ⁹
3, 4. Acrolein and acrylonitrile	G, FP-lined septum	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃, pH to 4-5¹⁰	14 days. ¹⁰
23, 30, 44, 49, 53, 77, 80, 81, 98, 100, 112. Phenols	G, FP-lined cap	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃	7 days until extraction, 40 days after extraction.
7, 38. Benzidines	G, FP-lined cap	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃ ⁵	7 days until extraction. ¹³
14, 17, 48, 50-52. Phthalate esters	G, FP-lined cap	Cool, ≤6°C ¹⁸	7 days until extraction, 40 days after extraction.
82-84. Nitrosamines	G, FP-lined cap	Cool, ≤6°C , store in dark, 0.008% Na₂S₂O₃⁵	7 days until extraction, 40 days after extraction.
88-94. PCBs	G, FP-lined cap	Cool, ≤6°C	1 year until extraction, 1 year after extraction.
54, 55, 75, 79. Nitroaromatics and isophorone	G, FP-lined cap	Cool, ≤6°C ¹⁸, store in dark, 0.008% Na₂S₂O₃⁵	7 days until extraction, 40 days after extraction.
1, 2, 5, 8-12, 32, 33, 58, 59, 74, 78, 99, 101. Polynuclear aromatic hydrocarbons	G, FP-lined cap	Cool, ≤6°C ¹⁸, store in dark, 0.008% Na₂S₂O₃⁵	7 days until extraction, 40 days after extraction.
15, 16, 21, 31, 87. Haloethers	G, FP-lined cap	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃ ⁵	7 days until extraction, 40 days after extraction.
29, 35-37, 63-65, 73, 107. Chlorinated hydrocarbons	G, FP-lined cap	Cool, ≤6°C	7 days until extraction, 40 days after extraction.
60-62, 66-72, 85, 86, 95-97, 102, 103. CDDs/CDFs	G	See footnote 11	See footnote 11.
Aqueous Samples: Field and Lab Preservation	G	Cool, ≤6°C ¹⁸, 0.008% Na₂S₂O₃ ⁵, pH <9	1 year.
Solids and Mixed-Phase Samples: Field Preservation	G	Cool, ≤6°C ¹⁸	7 days.
Tissue Samples: Field Preservation	G	Cool, ≤6°C ¹⁸	24 hours.
Solids, Mixed-Phase, and Tissue Samples: Lab Preservation	G	Freeze, ≤−10°C	1 year.
114-118. Alkylated phenols	G	Cool, <6°C, H₂SO₄ to pH <2	28 days until extraction, 40 days after extraction.
119. Adsorbable Organic Halides (AOX)	G	Cool, <6°C, 0.008% Na₂S₂O₃, HNO₃ to pH <2	Hold at least 3 days, but not more than 6 months.
120. Chlorinated Phenolics	G, FP-lined cap	Cool, <6°C, 0.008% Na₂S₂O₃, H₂SO₄ to pH <2	30 days until acetylation, 30 days after acetylation.
Table ID—Pesticides Tests
1-70. Pesticides	G, FP-lined cap	Cool, ≤6°C ¹⁸, pH 5-9⁵	7 days until extraction, 40 days after extraction.
Table IE—Radiological Tests
1-5. Alpha, beta, and radium	P, FP, G	HNO₃ to pH <2	6 months.
Table IH—Bacterial Tests
1, 2. Coliform, total, fecal	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃⁵	8 hours. ²²
3.E. coli	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours. ²²
4. Fecal streptococci	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours. ²²
5. Enterococci	PA, G	Cool, <10°C, 0.008% Na₂S₂O₃ ⁵	8 hours.²²
Table IH—Protozoan Tests
6. Cryptosporidium	LDPE; field filtration	1-10°C	96 hours. ²¹
7. Giardia	LDPE; field filtration	1-10°C	96 hours. ²¹
¹ ”P” is for polyethylene; “FP” is fluoropolymer (polytetrafluoroethylene [PTFE]; Teflon®), or other fluoropolymer, unless stated otherwise in this Table II; “G” is glass; “PA” is any plastic that is made of a sterilizable material (polypropylene or other autoclavable plastic); “LDPE” is low density polyethylene. ² Except where noted in this Table II and the method for the parameter, preserve each grab sample within 15 minutes of collection. For a composite sample collected with an automated sample (e.g., using a 24-hour composite sample; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), refrigerate the sample at ≤6°C during collection unless specified otherwise in this Table II or in the method(s). For a composite sample to be split into separate aliquots for preservation and/or analysis, maintain the sample at ≤6°C, unless specified otherwise in this Table II or in the method(s), until collection, splitting, and preservation is completed. Add the preservative to the sample container prior to sample collection when the preservative will not compromise the integrity of a grab sample, a composite sample, or aliquot split from a composite sample within 15 minutes of collection. If a composite measurement is required but a composite sample would compromise sample integrity, individual grab samples must be collected at prescribed time intervals (e.g., 4 samples over the course of a day, at 6-hour intervals). Grab samples must be analyzed separately and the concentrations averaged. Alternatively, grab samples may be collected in the field and composited in the laboratory if the compositing procedure produces results equivalent to results produced by arithmetic averaging of results of analysis of individual grab samples. For examples of laboratory compositing procedures, see EPA Method 1664 Rev. A (oil and grease) and the procedures at 40 CFR 141.24(f)(14)(iv) and (v) (volatile organics). ³ When any sample is to be shipped by common carrier or sent via the U.S. Postal Service, it must comply with the Department of Transportation Hazardous Materials Regulations (49 CFR part 172). The person offering such material for transportation is responsible for ensuring such compliance. For the preservation requirement of Table II, the Office of Hazardous Materials, Materials Transportation Bureau, Department of Transportation has determined that the Hazardous Materials Regulations do not apply to the following materials: Hydrochloric acid (HCl) in water solutions at concentrations of 0.04% by weight or less (pH about 1.96 or greater; Nitric acid (HNO₃) in water solutions at concentrations of 0.15% by weight or less (pH about 1.62 or greater); Sulfuric acid (H₂SO₄) in water solutions at concentrations of 0.35% by weight or less (pH about 1.15 or greater); and Sodium hydroxide (NaOH) in water solutions at concentrations of 0.080% by weight or less (pH about 12.30 or less). ⁴ Samples should be analyzed as soon as possible after collection. The times listed are the maximum times that samples may be held before the start of analysis and still be considered valid. Samples may be held for longer periods only if the permittee or monitoring laboratory have data on file to show that, for the specific types of samples under study, the analytes are stable for the longer time, and has received a variance from the Regional ATP Coordinator under §136.3(e). For a grab sample, the holding time begins at the time of collection. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), the holding time begins at the time of the end of collection of the composite sample. For a set of grab samples composited in the field or laboratory, the holding time begins at the time of collection of the last grab sample in the set. Some samples may not be stable for the maximum time period given in the table. A permittee or monitoring laboratory is obligated to hold the sample for a shorter time if it knows that a shorter time is necessary to maintain sample stability. See §136.3(e) for details. The date and time of collection of an individual grab sample is the date and time at which the sample is collected. For a set of grab samples to be composited, and that are all collected on the same calendar date, the date of collection is the date on which the samples are collected. For a set of grab samples to be composited, and that are collected across two calendar dates, the date of collection is the dates of the two days; e.g., November 14-15. For a composite sample collected automatically on a given date, the date of collection is the date on which the sample is collected. For a composite sample collected automatically, and that is collected across two calendar dates, the date of collection is the dates of the two days; e.g., November 14-15. For static-renewal toxicity tests, each grab or composite sample may also be used to prepare test solutions for renewal at 24 h, 48 h, and/or 72 h after first use, if stored at 0-6°C, with minimum head space. ⁵ ASTM D7365-09a specifies treatment options for samples containing oxidants (e.g., chlorine) for cyanide analyses. Also, Section 9060A of Standard Methods for the Examination of Water and Wastewater (23rd edition) addresses dechlorination procedures for microbiological analyses. [New Text] ⁶ Sampling, preservation and mitigating interferences in water samples for analysis of cyanide are described in ASTM D7365-09a (15). There may be interferences that are not mitigated by the analytical test methods or D7365-09a (15). Any technique for removal or suppression of interference may be employed, provided the laboratory demonstrates that it more accurately measures cyanide through quality control measures described in the analytical test method. Any removal or suppression technique not described in D7365-09a (15) or the analytical test method must be documented along with supporting data. ⁷ For dissolved metals, filter grab samples within 15 minutes of collection and before adding preservatives. For a composite sample collected with an automated sampler (e.g., using a 24-hour composite sampler; see 40 CFR 122.21(g)(7)(i) or 40 CFR part 403, appendix E), filter the sample within 15 minutes after completion of collection and before adding preservatives. If it is known or suspected that dissolved sample integrity will be compromised during collection of a composite sample collected automatically over time (e.g., by interchange of a metal between dissolved and suspended forms), collect and filter grab samples to be composited (footnote 2) in place of a composite sample collected automatically. ⁸ Guidance applies to samples to be analyzed by GC, LC, or GC/MS for specific compounds. ⁹ If the sample is not adjusted to pH 2, then the sample must be analyzed within seven days of sampling. ¹⁰ The pH adjustment is not required if acrolein will not be measured. Samples for acrolein receiving no pH adjustment must be analyzed within 3 days of sampling. ¹¹ When the extractable analytes of concern fall within a single chemical category, the specified preservative and maximum holding times should be observed for optimum safeguard of sample integrity (i.e., use all necessary preservatives and hold for the shortest time listed). When the analytes of concern fall within two or more chemical categories, the sample may be preserved by cooling to ≤6°C, reducing residual chlorine with 0.008% sodium thiosulfate, storing in the dark, and adjusting the pH to 6-9; samples preserved in this manner may be held for seven days before extraction and for forty days after extraction. Exceptions to this optional preservation and holding time procedure are noted in footnote 5 (regarding the requirement for thiosulfate reduction), and footnotes 12, 13 (regarding the analysis of benzidine). ¹² If 1,2-diphenylhydrazine is likely to be present, adjust the pH of the sample to 4.0 ± 0.2 to prevent rearrangement to benzidine. ¹³ Extracts may be stored up to 30 days at <0°C. ¹⁴For the analysis of diphenylnitrosamine, add 0.008% Na₂S₂O₃ and adjust pH to 7-10 with NaOH within 24 hours of sampling. ¹⁵ The pH adjustment may be performed upon receipt at the laboratory and may be omitted if the samples are extracted within 72 hours of collection. For the analysis of aldrin, add 0.008% Na₂S₂O₃. ¹⁶ Place sufficient ice with the samples in the shipping container to ensure that ice is still present when the samples arrive at the laboratory. However, even if ice is present when the samples arrive, immediately measure the temperature of the samples and confirm that the preservation temperature maximum has not been exceeded. In the isolated cases where it can be documented that this holding temperature cannot be met, the permittee can be given the option of on-site testing or can request a variance. The request for a variance should include supportive data which show that the toxicity of the effluent samples is not reduced because of the increased holding temperature. Aqueous samples must not be frozen. Hand-delivered samples used on the day of collection do not need to be cooled to 0 to 6°C prior to test initiation. ¹⁷ Samples collected for the determination of trace level mercury (<100 ng/L) using EPA Method 1631 must be collected in tightly-capped fluoropolymer or glass bottles and preserved with BrCl or HCl solution within 48 hours of sample collection. The time to preservation may be extended to 28 days if a sample is oxidized in the sample bottle. A sample collected for dissolved trace level mercury should be filtered in the laboratory within 24 hours of the time of collection. However, if circumstances preclude overnight shipment, the sample should be filtered in a designated clean area in the field in accordance with procedures given in Method 1669. If sample integrity will not be maintained by shipment to and filtration in the laboratory, the sample must be filtered in a designated clean area in the field within the time period necessary to maintain sample integrity. A sample that has been collected for determination of total or dissolved trace level mercury must be analyzed within 90 days of sample collection. ¹⁸ Aqueous samples must be preserved at ≤6°C, and should not be frozen unless data demonstrating that sample freezing does not adversely impact sample integrity is maintained on file and accepted as valid by the regulatory authority. Also, for purposes of NPDES monitoring, the specification of “≤°C” is used in place of the “4°C” and “<4°C” sample temperature requirements listed in some methods. It is not necessary to measure the sample temperature to three significant figures (1/100th of 1 degree); rather, three significant figures are specified so that rounding down to 6°C may not be used to meet the ≤6°C requirement. The preservation temperature does not apply to samples that are analyzed immediately (less than 15 minutes). ¹⁹ An aqueous sample may be collected and shipped without acid preservation. However, acid must be added at least 24 hours before analysis to dissolve any metals that adsorb to the container walls. If the sample must be analyzed within 24 hours of collection, add the acid immediately (see footnote 2). Soil and sediment samples do not need to be preserved with acid. The allowances in this footnote supersede the preservation and holding time requirements in the approved metals methods. ²⁰ To achieve the 28-day holding time, use the ammonium sulfate buffer solution specified in EPA Method 218.6. The allowance in this footnote supersedes preservation and holding time requirements in the approved hexavalent chromium methods, unless this supersession would compromise the measurement, in which case requirements in the method must be followed. ²¹Holding time is calculated from time of sample collection to elution for samples shipped to the laboratory in bulk and calculated from the time of sample filtration to elution for samples filtered in the field. ²² Sample analysis should begin as soon as possible after receipt; sample incubation must be started no later than 8 hours from time of collection. ²³ For fecal coliform samples for sewage sludge (biosolids) only, the holding time is extended to 24 hours for the following sample types using either EPA Method 1680 (LTB-EC) or 1681 (A-1): Class A composted, Class B aerobically digested, and Class B anaerobically digested. ²⁴ The immediate filtration requirement in orthophosphate measurement is to assess the dissolved or bio-available form of orthophosphorus (i.e., that which passes through a 0.45-micron filter), hence the requirement to filter the sample immediately upon collection (i.e., within 15 minutes of collection).

Table IB—List of Approved Inorganic Test Procedures
Parameter	Methodology ⁵⁸	EPA ⁵²	Standard methods ⁸⁴	ASTM	USGS/AOAC/other
1. Acidity, as CaCO₃, mg/L	Electrometric endpoint or phenolphthalein endpoint		2310 B-2011	D1067-16	I-1020-85.²
2. Alkalinity, as CaCO₃, mg/L	Electrometric or Colorimetric titration to pH 4.5, Manual		2320 B-2011	D1067-16	973.43,³ I-1030-85.²
	Automatic	310.2 (Rev. 1974) ¹			I-2030-85.²
3. Aluminum—Total,⁴ mg/L	Digestion,⁴ followed by any of the following:
	AA direct aspiration ³⁶		3111 D-2011 or 3111 E-2011		I-3051-85.²
	AA furnace		3113 B-2010.
	STGFAA	200.9, Rev. 2.2 (1994)/
	ICP/AES ³⁶	200.5, Rev 4.2 (2003); ⁶⁸ 200.7, Rev. 4.4 (1994)	3120 B-2011	D1976-12	I-4471-97.⁵⁰
	ICP/MS	200.8, Rev. 5.4 (1994)	3125 B-2011	D5673-16	993.14,³ I-4472-97.⁸¹
	Direct Current Plasma (DCP) ³⁶			D4190-15	See footnote.³⁴
	Colorimetric (Eriochrome cyanine R)		3500-Al B-2011.
4. Ammonia (as N), mg/L	Manual distillation ⁶ or gas diffusion (pH > 11), followed by any of the following:	350.1, Rev. 2.0 (1993)	4500-NH₃ B-2011		973.49.³
	Nesslerization			D1426-15 (A)	973.49,³ I-3520-85.²
	Titration		4500-NH₃ C-2011.
	Electrode		4500-NH₃ D-2011 or E-2011	D1426-15 (B).
	Manual phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods		4500-NH₃ F-2011		See footnote.⁶⁰
	Automated phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods	350.1,³⁰ Rev. 2.0 (1993)	4500-NH₃ G-2011 4500-NH₃ H-2011		I-4523-85,² I-2522-90.⁸⁰
	Automated electrode				See footnote.⁷
	Ion Chromatography			D6919-17.
	Automated gas diffusion, followed by conductivity cell analysis				Timberline Ammonia-001.⁷⁴
	Automated gas diffusion followed by fluorescence detector analysis				FIAlab100.⁸²
5. Antimony—Total,⁴ mg/L	Digestion,⁴ followed by any of the following:
	AA direct aspiration ³⁶		3111 B-2011.
	AA furnace		3113 B-2010.
	STGFAA	200.9, Rev. 2.2 (1994).
	ICP/AES ³⁶	200.5, Rev 4.2 (2003); ⁶⁸ 200.7, Rev. 4.4 (1994)	3120 B-2011	D1976-12.
	ICP/MS	200.8, Rev. 5.4 (1994)	3125 B-2011	D5673-16	993.14,³ I-4472-97.⁸¹
6. Arsenic-Total,⁴ mg/L	Digestion,⁴ followed by any of the following:	206.5 (Issued 1978) ¹.
	AA gaseous hydride		3114 B-2011 or 3114 C-2011	D2972-15 (B)	I-3062-85.²
	AA furnace		3113 B-2010	D2972-15 (C)	I-4063-98.⁴⁹
	STGFAA	200.9, Rev. 2.2 (1994).
	ICP/AES ³⁶	200.5, Rev 4.2 (2003); ⁶⁸ 200.7, Rev. 4.4 (1994)	3120 B-2011	D1976-12.
	ICP/MS	200.8, Rev. 5.4 (1994)	3125 B-2011	D5673-16	993.14,³ I-4020-05.⁷⁰
	Colorimetric (SDDC)		3500-As B-2011	D2972-15 (A)	I-3060-85.²
7. Barium-Total,⁴ mg/L	Digestion,⁴ followed by any of the following:
	AA direct aspiration ³⁶		3111 D-2011		I-3084-85.²
	AA furnace		3113 B-2010	D4382-18.
	ICP/AES ³⁶	200.5, Rev 4.2 (2003); ⁶⁸ 200.7, Rev. 4.4 (1994)	3120 B-2011		I-4471-97.⁵⁰
	ICP/MS	200.8, Rev. 5.4 (1994)	3125 B-2011	D5673-16	993.14,³ I-4472-97.⁸¹
	DCP ³⁶				See footnote.³⁴

...

§136.3 Identification of test procedures.